Usp15 (BC050042) Mouse Untagged Clone

CAT#: MC205983

Usp15 (untagged) - Mouse ubiquitin specific peptidase 15 (cDNA clone MGC:61019 IMAGE:30022307), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp15
• Synonyms: mKIAA0529
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050042
  GCTACGGCTAGTGGAAGAAGATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGC GACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAG TGGAAAAAATATGTTGGCTTTGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGAC CCATCGATAACTCTGGACTTCTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTT GGATTACATACTCTTGCCAACTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAG GAGCCAATAGCAAGAAAGGTGGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCA CAGAACTGAAGCTCTGTGAGAACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACAC AATAGATACGATTGAGAAGGAAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGG AACAAGTATGTGAGCAACACATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTAT ACCAAGGACAGGTGTTAGTGATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCC TAAGTCCCCAGGTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTAT AACAACATCAACAACAGAAATGTGAAAAACTCAAATTATTGTCTCCCGTCATATACTGCTTATAAGAACT ATGATTATTCAGAACCTGGAAGAAACAATGAACAGCCAGGCCTGTGTGGCTTAAGTAACTTGGGAAATAC GTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAACACACCTCCACTTACTGAATATTTCCTCAATGAT AAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCATACGCTG AACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTACGTCACTCCAAGAGCATTCAAGACACAGGTAGG GCGCTTCGCACCTCAGTTCTCTGGATATCAGCAGCAGGACTGCCAAGAACTACTGGCTTTCCTGTTGGAT GGACTGCACGAGGACCTGAACAGAATTAGGAAAAAACCCTATATCCAGTTAAAAGATGCTGATGGCAGGC CAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCATTTAAAGCGAAATGATTCCATCATAGTAGACAT ATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCTGAGTGTGCTAAGATCTCGGTGACCTTTGACCCT TTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAGAACGTAGCTTGGAAGTTTATTTAGTTAGAATGG ATCCACTTGCTAAACCTATGCAATACAAAGTTATTGTGCCCAAAATTGGAAATATACTAGATCTTTGTAC AGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAGATGATAGTCACTGACATATACAATCATAGATTT CACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTATTATGGAGCGGGATGATATTTACGTGTTTGAAA TTAACATCAACAGGGCAGAAGACACAGAGCATGTAGTCATTCCTGTTTGCCTAAGAGAAAAATTCAGACA CTCAAGTTACACGCACCACACTGGCTCTTCACTGTTCGGCCAGCCTTTTCTTATGGCTATACCACGAAAC AATACTGAAGATAAACTCTATAATCTTCTACTCTTGAGAATGTGCCGGTATGTCAAAATGTCTACTGAAA CTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGACCAGAATATTAATGGGAATGGGCCAAATGGCCT ACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGACGAGCCAGGTGACGAGTCCAGCCAGGATCAAGAG CTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATTCAGTCGGAGGAGATAACGATTCTGAAAATGGAT TGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGGACACAAAAAGCGCTTGTTTACATTCCAGTTCAA CAACTTAGGCAACAATGACATCAACTACATCAAAGACGACACCAGTCACATCCGATTCGATGACAGGCAG CTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACTGGGACCCTGACTTGAAGAAGAGATACTTTGATG AGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGTGGAATACAAACCTCCAAAAAGGCCCTTTGTGAA GTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAGGAGTTGGGTGCTGAGGACCCCTGGTATTGTCCA AATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAGATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGC ATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGACAAGCTAGATACATTAGTCGATTTTCCCATCAG TGACTTGGATATGTCAGAATTCCTAATTAACCCAAATGCAGGTCCTTGCCGCTATAATTTGATTGCTGTT TCCAACCACTATGGAGGGATGGGAGGAGGACATTATACTGCTTTTGCAAAAAACAAAGATGATGGGAAGT GGTACTATTTCGATGACAGTAGCGTCTCCACTGCATCTGAAGACCAAATCGTGTCCAAGGCAGCGTACGT GCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACTGGCTTCTTCCCTCTTGACCGAGAAACTAAAGGT GCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACGAAGACAGCAATGATAATGACAATGACCTAGAAA ATGAAAACTGTATGCACACTAATTAACGAAAGTGCAAGAAGCCATAGGAGAGACTTTCCTGCTGGTGGTG TCTATGGAGATGAAGTTATCACCACATGAAACAGAAGTCTGAGATGAGGAGTTTCAGATACCCGAATGTA AATCCTTTATCAGATTTTAACTTGTGCAGTACTTGAAGTGAAACACAATGAAAACTTTAACAGAAATTGT CTCTTAATACATTTACAGTCTTGTATTTACAAGCTAAATATATATAGGAAATCACAAATAAATCCCTTTT AAGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC050042
• Insert Size: 2946 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050042, AAH50042
• RefSeq Size: 3253 bp
• RefSeq ORF: 2946 bp
• Locus ID: 14479
• Cytogenetics: 10 D2
• Gene Summary: The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014]