Akt3 (BC066861) Mouse Untagged Clone

CAT#: MC205969

Akt3 (untagged) - Mouse thymoma viral proto-oncogene 3 (cDNA clone MGC:76721 IMAGE:30089997), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akt3
• Synonyms: AI851531; D930002M15Rik; Nmf350
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066861
  GCAAAGCACCATTTCTCCAAGTTGGGGGGCTCAGAGGGGAGCCATCATGAGCGATGTTACCATTGTGAAA GAAGGTTGGGTTCAGAAGAGGGGAGAATATATAAAAAACTGGAGGCCAAGATACTTCCTTTTGAAGACAG ATGGCTCATTCATAGGCTATAAGGAGAAACCTCAAGATGTGGACTTACCTTATCCCCTCAACAACTTCTC AGTGGCAAAATGTCAGTTAATGAAAACAGAACGACCAAAGCCAAATACATTTATTATCAGATGTCTTCAG TGGACCACTGTTATAGAGAGAACATTTCATGTAGATACACCAGAGGAAAGAGAAGAGTGGACGGAAGCTA TCCAAGCCGTAGCCGACCGATTGCAGAGGCAAGAGGAGGAGAGGATGAATTGTAGCCCAGCCTCACAGAT TGATAATATAGGAGAAGAAGAGATGGATGCGTCTACAACCCATCATAAAAGAAAGACGATGAATGATTTT GACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAA AATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTTAC TGAAAGCAGAGTACTAAAGAACACCAGACATCCATTTTTAACATCCTTGAAATATTCCTTCCAGACAAAA GACCGTTTGTGTTTTGTGATGGAATATGTTAATGGCGGAGAGCTGTTTTTCCATTTGTCGAGAGAGCGAG TGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTCTGCTTTGGACTATCTACATTCTGG AAAGATTGTGTACCGTGATCTCAAGTTGGAGAATTTGATGCTAGATAAGGATGGCCATATAAAAATTACG GATTTTGGGCTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGAAGACATTCTGTGGCACACCAGAGT ACCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCCGTGGACTGGTGGGGCTTAGGTGTTGT CATGTATGAAATGATGTGTGGAAGGTTGCCTTTCTACAACCAGGATCATGAGAAACTCTTTGAATTAATA CTAATGGAAGACATTAAATTCCCCCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGA TAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATGCAAAAGAAATCATGAGGCATAGTTTTTT TTCTGGAGTAAACTGGCAAGATGTATATGACAAAAAGCTTGTACCTCCTTTTAAGCCTCAAGTAACATCT GAAACAGACACCCGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAGT ATGACGACGACGGCATGGACGGCATGGACAGCGAGCGGCGGCCACACTTCCCTCAGTTCTCCTACTCTGC AAGCGGACGGGAATAAGTTCCTTTCAGTCTGTTTCTACACTGTCATCTTAGACTTTGCCTGAGACTGATT CCTGGACATCTCTACCAGTCCTCGCTCTTACAGTTAGCAGGGGCACCTTCTGACATCCCTGACCAGCCAA GGGTCTTCACCCTCACCACCTTTCACTCACATGAAACCATATACACAGACACTCCAGTTTTGTTTTTGCA TGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCTACTGTCTTACTATTATAGCAACCTTCAGA AGTAATTTCACAATCTTTGGGAGTCATGAGCCCATTGTTCATTTGTGCATCAAGTGTCATCTTTTGGTTT TTGGTTTTCCCTAGCAGTGAAGGCTAAATGAGATACACTGATTCTAGGTACATTGTTAACTTTCTAGGAG ATAAATCAAGAACTAATTAGACTAAGAAGATTTAGTTTATATTTCTGAACAAGCAATTGTTGAAGGGTGG TGGTGTGTGTATGTAAGAGTTTAGCAGTGTATGTCTGACCAGCACATGAAGTGTGTATCACTGACATTTG GCTGGAAAATCTTAGGAAACATTGAGAGGACTGAAGGGCCAATGAGATACACATTTTTTTTTTTTTAAAT CCTGCTGGCTATTGTGGAGGAAGCCCAGTGCCACCCATGGATGCCCAGGTTTCAAGGGCAAATGGACATT GTTCAACCAAGTACTGACGGTGCAGAGTCCCCTAGAGAGTCTTAGGATCTACTTTGTACCAGAGAGTATT TTCCCCTGGGCTTGGCAACCTTTTTCCCTTCTTTATATTTATCACCTCTGATGGCTGAAGAATGTAGACA GTATAATGATCCTGCTGTCGCCAAAATCTATACACCAAGGTGAACAGGTGTTTGCCTTACCAGTTTTGGA GTTTTTACTTTCAGTTATAGGTAAATTAGCTTTTTCTCAGATGTTAAAACCTTGAATGTCCTTTATGGTT TTGTTTATATTACAGTAGTATTTATTTTTTAGTGGTAAGAATTGTGTTGTCATGTTAGCAAGCGCAGCTC CAATTCACAGATCATTGCCTGCGTTTTCTTTTGACCCATGTGCAAGGAATGTACACACCCATTAGAATCA TGCACTTTTTCTCCTATGTTAAAAACAATGGTAAGGCTCTGGGACTGTATCTGTTGGTTAGAATCTGGCT AGAATCTCTGGGAGAAGAGATGCTGTGATTTAACCCCTTGGAGCTGAAATGAGAGCATCCCCAACTACCC AGTGCCAAGGGATGGATGTGAGAGGATAGTCTGGCAGAAAGTAATGGAGAAAGCTGAGACCCAAATGATG ATGAGAAATGAACTCTACTCGGCAAAATGAACACATCACAAGTATTCACACAGTAGGAAGTAAAGGATAA GAAGCTGATGTGTTTATTCTTATCAAGGTACATTCATTACTGAATCAAGCCATCAGGGAAAACAACAAAA CAAAACACCTCTAGCTTTTATTTTTCTGTACATATTCATGTCTCCCAACCCAAACATTTCTCACTGAGAG GGGACATGTATGCAAACCTCATCTTTCTCCTTCATTAATGATGATCTTCAGATTAACCCTTTGGTGCTAG GAGCTGACACTTTCCAAAGCAGACTATGACGTCCAAGGATCAAAGCCTCTCTTGCAGCAAGCAAGAGCAA CTGCTGCTCCAAGGCGTGATCATGGAAGTGGAAGACCACAGTGTGACACAGTGGTACTGCATTGTCTTTT TCAGTAGGAAGTCATGTTCTCATGCAAGTGTCTTGGCCTCTAGGCTCAGACTTAGAGAAGCAGAGCCGCT GCTGGCAGTGGCTGGCCGCTGGGACTGCTGGGGTGTAAAGGGCATTTTCCTAAGGCAGCTAAGACAGACT CAGACATAGATTTTTGTCCTAATTTTTCATGTTTCTACCTGGGTGAGTTTACCCTCAGTGGGAAGTAGGA AGTTAAACAGGTGTATTCTTACATCTGCTGTCTTCTGTTTTCCTACATAGAAGTATGTTGAATGTTGCAC ATTGACAAGGAAGTAGATTAGATGGTAAGTCCTTTTAAGTTACCCTGCATCCATTCTGTAAGAAAGGAGG AGGAGAGGAAGAAAAGCAAGCTGAGCCTGGCCGATGTTCAGAACATTTACTCCCTCAGAGGGAGCAGACA CTGTACACTAAGCACACAGCTTCTTTTGTTTTGTCTCTCCTTCCAAGTCTTAAGGTAATTTCATGACCTT GGCCAAGGATACTTTGTGAAGATTAATGAGGGGACATTGACAATGCCTCAGGCTGGCCACTCCTCACACT GGCCCTTGGTCCCCAGCCATTCAAGGGGTTACTCATCCCATCCCTGACTACCTGAGTGTCACTACAGGTG GGTTCTTGGCTTTGAGTTCAAGAATTCTTTCCTTACACCCTCCACCAGATGTCAGTGGAAAGGCAGAAGT AGAACCGAGGTGTGGGAGGAGATTCTAAAAGCTTGTGTTGCCCAGGCCTCTAGCTTAGGGTTCTGGCAAT TGAGACGGCCTGAAACCTGACAGCATCATGTCCGGGCAGCTCTGGCTTTGTGAGTCCAGCTCCTACCAAC TCCACCTTGACCTCTTGTCTCCATGCAGGGCTGTCTGGGAGGAAACTGGCCACTTCTGCTCAGACTGCTG CCAGCGCTCTCACAGCTTTGCTCTCTACACTAATGACATAGATTATTCCAGTATTGTTCCCATTTCCCAC CTGACCTCCAGCTTCTCGGAGCTGACTTCTTGCAGGGGCCACATGCTTCTTTCCCTCACTAACTGCAGGG TCTCCACCACACCTCAGTGTACACACTTTGCTGCTACCGTCTGTACTGTCTACATCACGGTTCCCTTAGC TTGCTCCTGGTAGTGCATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAAAGGAAACCTCT GAATTGGTCATCGAGTCACCTCCCTGTAGATTTGTAGTCTGTGACATATTTCGACTTTCTAGTCCTGCTA GATCCATATAAGATCTGGTCATCTGGTTAAGGGTTTGAAGCAGACATAGCTATGAACCCGGATGGTATCA TCACTGTCACGGCTGGCCACACTACAGATGTTTATGTCCTTACGTTTATGACCCACAGGGTGTGAAGTAA CTTCCAAGAACCTGATTTTGTACACTGTCCACTCTCAGCATCTCACACTCTCTGATAGGGACACACATTA CTTCCTTCGTACAGACAGCTTAAATAAAGCCCTATGTCAATCTGCAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC066861
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066861, AAH66861
• RefSeq Size: 4751 bp
• RefSeq ORF: 1440 bp
• Locus ID: 23797
• Cytogenetics: 1 H4
• Gene Summary: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.[UniProtKB/Swiss-Prot Function]