Gne (NM_015828) Mouse Untagged Clone

CAT#: MC205887

Gne (untagged) - Mouse glucosamine (Gne), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gne
• Synonyms: 2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051254
  GCTAAACCAGAGGCCAGACGGCAGCTCAGGAGTCCGACCACACCTCAGGAAACAGCTGTGCCACAGGATG GAAACACACGCGCATCTCCACAGGGAGCAGAGCTACGCAGGACCTCATGAACTCTATTTTAAGAAACTCT CAAGTAAAAAGAAGCAAGTCATGGAGAAGAACGGGAACAACCGAAAGCTCCGGGTTTGCGTTGCCACCTG CAACCGAGCTGACTACTCCAAACTGGCCCCGATCATGTTCGGCATCAAGACAGAGCCCGCGTTCTTTGAG TTGGACGTGGTGGTGCTCGGCTCCCACCTGATTGACGACTATGGAAACACATACCGCATGATTGAGCAAG ATGACTTTGACATTAACACCAGGCTCCACACGATTGTTAGAGGGGAAGATGAAGCGGCCATGGTAGAGTC GGTAGGCCTAGCGCTCGTGAAGCTACCGGACGTCCTCAATCGCCTGAAGCCCGACATCATGATTGTTCAC GGAGACCGATTTGACGCCCTTGCTCTGGCTACGTCTGCTGCCTTGATGAACATCCGCATCCTTCACATTG AAGGAGGCGAGGTCAGCGGGACCATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCA TGTGTGCTGCACTAGAAGTGCAGAGCAGCACCTGATCTCTATGTGCGAGGACCACGACCGCATCCTGTTG GCAGGCTGCCCTTCCTATGACAAACTGCTCTCCGCCAAGAACAAAGACTATATGAGCATCATTCGGATGT GGCTAGGCGATGATGTAAAATGTAAGGATTACATCGTTGCCCTGCAGCATCCCGTGACCACTGACATTAA GCATTCCATAAAGATGTTTGAGCTAACACTGGATGCCCTGATCTCGTTTAACAAGAGGACCCTAGTTCTG TTTCCAAATATCGATGCAGGCAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACC CCAATTTCCGTGCAGTCAAGCACGTCCCGTTTGACCAGTTCATACAGCTGGTCGCCCACGCTGGCTGCAT GATTGGGAATAGCAGCTGCGGCGTGCGAGAGGTTGGCGCTTTCGGAACACCCGTGATCAACCTGGGCACA AGGCAGATAGGAAGAGAAACCGGGGAGAATGTTCTTCATGTCAGGGATGCTGACACCCAAGATAAAATAT TGCAAGCACTACACCTCCAGTTCGGCAAACAGTACCCTTGCTCAAAGATATATGGGGATGGGAATGCTGT TCCAAGGATTTTAAAGTTTCTCAAATCCATTGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCC CCTGTAAAGGAGAACATCTCTCAAGACATTGACCACATCCTGGAAACTCTGAGTGCCTTGGCTGTTGATC TTGGCGGGACAAACCTGAGGGTGGCAATAGTTAGCATGAAGGGTGAAATCGTTAAGAAGTACACTCAGTT CAACCCTAAAACCTATGAAGAAAGGATTAGTTTAATCCTGCAGATGTGTGTGGAAGCTGCCGCGGAAGCT GTGAAACTCAATTGCAGAATTCTGGGAGTAGGCATCTCCACAGGTGGCCGCGTGAATCCCCAGGAAGGAG TTGTGCTGCATTCAACCAAGCTGATCCAGGAATGGAACTCCGTGGACCTCAGGACACCCCTCTCCGACAC CCTGCATCTCCCCGTGTGGGTGGACAATGACGGCAACTGTGCCGCCATGGCAGAGAGGAAGTTCGGCCAA GGAAAAGGACAGGAGAACTTCGTGACGCTCATCACGGGGACAGGGATCGGTGGGGGGATCATCCACCAGC ACGAACTGATCCACGGCAGCTCCTTCTGCGCGGCGGAGCTCGGCCATCTCGTGGTGTCCCTGGACGGTCC TGACTGCTCCTGTGGAAGCCATGGGTGCATCGAAGCGTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCA AAGAAACTCCATGATGAGGACCTGCTCTTGGTGGAAGGGATGTCAGTACCAAAAGACGAAGCTGTGGGTG CCCTCCATCTCATCCAGGCTGCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGAACAGCTGGAAC TGCTTTGGGACTTGGGGTTGTGAACATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTC CTGGCCAGTCACTACATCCACATCGTGAAGGACGTCATCCGCCAGCAAGCCTTGTCCTCCGTGCAGGATG TGGACGTGGTGGTCTCAGACTTGGTGGACCCGGCCCTGCTTGGCGCAGCCAGCATGGTTCTGGACTACAC AACGCGCAGGATCCACTAGGTCTCCCGGGAACGGACACGGACAGAGACTCGGGAGCTGCTTAGAGTGGAA CCATGCTCTTCTAGATCAGTGTTTCTGCGAAGGCAAATTTGGGGGGAGGGCTGCTGAGACAGCTCAGTGG TTAAGAGCCTGCCCTGCTCCTGCCAGTCCCCAGCACCCATGTCAGGCAGCTCAGCTGCCTGGAAGCCAAG CTCCAGGGGACCCAATGCCTCTCTGCCGGGGGCAGCTGCACTCAGATGTACATACCCCTCTCCACACACA TACAAATAAAGCTTATTTTTCAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_015828
• Insert Size: 2169 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051254, AAH51254
• RefSeq Size: 2654 bp
• RefSeq ORF: 2169 bp
• Locus ID: 50798
• UniProt ID: Q91WG8
• Cytogenetics: 4 B1
• Gene Summary: Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Required for normal sialylation in hematopoietic cells (By similarity). Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Plays an essential role in early development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) encodes the longest protein (isoform 2). This variant has been called GNE2 in the scientific literature. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The use of an alternative upstream start codon, would result in a protein that is 31 aa longer at the N-terminal.