Dnajc10 (NM_024181) Mouse Untagged Clone

CAT#: MC205861

Dnajc10 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnajc10
• Synonyms: 1200006L06Rik; D2Ertd706e; ERdj5; JPDI
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC033461
  GCGTTCGCAGGCAGCGTCCGGTCCTGCCGGGTCCGGAGCTCGGCGGCCTGAGGATGGGGCAGTAGCTGAG GACGCCGAGGTTAACATTTCTGAGCTGTGTTCCCGAGATGCTTGATTGACCCTTGAAGTTATGTGGACAG AGATTCTCACGTGTGTCTATTTTAACCACCGGACCCAGCATTGTATCTTAATGTTCACATAAGTCAGAAC TTTCATAAGAAAGAGAATGGGAGTCTGGTTAAACAAAGATGACTTTATCAGAGACTTGAAGAGGATCAGT CTCTGTCTTCTGATACTGTACGTGGTCGTTGTAGTGGGCACAGATCAGAATTTTTACAGTTTACTTGGAG TATCTAAAACTGCAAGCAGTAGAGAAATAAGACAAGCCTTTAAGAAATTAGCACTGAAGTTACATCCTGA TAAAAACCCGAATAACCCAAATGCACATGGTGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCAAA GATGAAGATCTACGGAAAAAGTATGACAAATATGGAGAAAAGGGACTTGAAGATAATCAAGGAGGCCAGT ATGAGAGCTGGAGCTACTACCGTTACGATTTCGGTATTTACGATGATGATCCTGAAATCATAACATTAGA AAGAAGAGAATTTGATGCTGCTGTCAACTCTGGAGAACTGTGGTTTGTAAACTTTTACTCCCCAGGATGT TCGCATTGCCATGATTTAGCCCCCACTTGGAGAGAATTTGCTAAAGAAGTGGATGGGTTGCTTCGAATCG GAGCTGTCAACTGTGGTGATGATCGGATGCTCTGCCGAATGAAAGGAGTTAACAGCTACCCCAGCCTCTT CATCTTTAGGTCTGGAATGGCTGCAGTGAAGTACAATGGAGATCGGTCAAAGGAGAGCTTAGTGGCCTTC GCCATGCAGCACGTCAGGAGCACAGTGACAGAGCTGTCAACAGGAAATTTTGTTAATGCCATAGAAACTG CTTTTGCTGCTGGTGTTGGCTGGCTGATCACATTCTGCTCTAAAGGAGAAGATTGTTTGACGTCACAGAC CCGACTCAGGCTTAGTGGCATGTTGGATGGCCTTGTTAACGTGGGATGGGTGGACTGTGACGCCCAGGAC AGCCTCTGCAAGAGCCTGGACACCACAGCCAGCACCACGGCTTATTTCCCTCCTGGAGCCACTTTGAATG ACAGGGGAAAATCCAGTGTGTTGTTTCTTAATTCATTGGATGCTAAGGAAATATACATGGAAATCATACA TAATCTTCCAGACTTTGAACTGCTATCAGCAAATCAACTAGAGGATCGTTTGGCACATCATCGCTGGCTT GTATTTTTTCATTTTGGAAAAAATGAAAACGCAAATGATCCTGAGTTGAAAAAACTGAAAACTCTACTTA AAAATGAGCATATTCAAGTTGGAAGGTTTGATTGCTCTTCTGCACCGGGCATCTGCTCTGATCTTTATGT CTTCCAGCCCTGCCTAGCAGTGTTTAAAGGACAAGGAACCAAAGAATATGAAACTCATCATGGAAAGAAG ATTCTATACGATATACTTGCTTTTGCCAAAGAAAGTGTTAATTCTCATGTTACCACACTTGGACCTCAGA ACTTCCCTGCAAGTGACAAGGAACCATGGCTTGTTGACTTCTTTGCCCCTTGGTGTCCGCCATGTCGTGC GCTTTTGCCGGAGTTACGGAAAGCATCAACACTGCTCTACGGTCAACTCAAAGTTGGTACCTTAGACTGT ACAATTCATGAAGGACTCTGCAACATGTATAACATCCAAGCTTATCCAACGACAGTGGTATTCAATCAGT CCAGTATTCATGAGTATGAAGGACATCACTCTGCGGAACAGATCTTGGAGTTCATAGAGGATCTCAGAAA CCCTTCAGTGGTCTCTCTTACACCCAGCACTTTCAATGAGCTGGTTAAACAGAGAAAGCATGATGAAGTC TGGATGGTCGATTTCTATTCTCCATGGTGTCATCCCTGTCAGGTCTTGATGCCAGAATGGAAACGCATGG CCCGGACGTTAACTGGACTGATCAATGTAGGCAGTGTGGACTGCCAACAGTATCATTCTTTTTGTACTCA AGAAAATGTTCAAAGATACCCCGAGATAAGATTTTATCCCCAAAAGTCAAGTAAAGCCTATCAGTATCAT AGTTACAATGGCTGGAATAGGGATGCGTATTCCCTGAGAAGCTGGGGTCTTGGATTTTTGCCCCAAGCAT CCATAGATTTAACACCTCAGACTTTCAATGAAAAAGTTCTACAAGGGAAAACTCACTGGGTGGTTGATTT CTATGCTCCTTGGTGTGGACCTTGCCAGAATTTTGCTCCTGAGTTTGAACTCTTGGCTAGGATGATCAAA GGAAAAGTGAGAGCCGGGAAAGTGGACTGTCAGGCTTACCCCCAGACGTGCCAGAAAGCCGGCATCAAAG CCTACCCCAGTGTCAAACTGTACCAGTACGAGAGAGCAAAGAAAAGTATTTGGGAAGAGCAAATAAATTC CAGAGATGCAAAAACTATTGCTGCCTTAATTTATGGAAAATTGGAAACGCTCCAAAGCCAAGTAAAAAGA AATAAGGATGAGCTTTGATGGTGTTGAAGAAAAAGCATGTAAGGAAAAGAATCTGGAGAACGGCATCAGA AGATCCCTTTCCCAAGTGACGATGCTCCTGAGGAAGCAGATGCACGTGGCCTGGGCCTCGCAGTTGCCTG TGTACACAGAGCTCCTGTGCGGCGCTGCGGGCTGCAGATGCTTCCTGGAGAGCCAGGCAGCGCCATCTGA GACTGCAGTCCACATATGGTTCTGTGTTGTGTCTTTGTTGTTGTTGTTAATCCTTTTAGAAATACAAACA TCAGTCTTAGCTCAGGGCCATATAGAAATACTCCACAGGCCAAGTCCAGCCCAGGGACTAGTTTGCTGAC CCCAGAACTGATGTTTGGACACCTGGCCCTGCACGTGGCTCTGTTACCTTCAGACTGTACAAAGTAGACT TGGGTTTTCACTTGTAAATTTCTGATGCGCTTGAAAAGAACTGACTTGCTTTTCAGTCACCTCTTCTAAT TCTGTCTATGACCATATATTTTATTTTGACTATTTTCACCACATAGGAAAATAAAAATTACCCATTTTTC CTACTATGTACTACTGTATAGACAGAGGCTTTCTTGTAAGAGTTGAAGAGTTCTTAATTTATCACAACAG TAAGTGTAAAAATCCTGTAAGCTGCTGTCCCAGGAACAAGGTAAGAAGAAGTATTTACAGCAGTTGATGG GCTGTGTGGCTGTCACAGGTCTAAGCCTGTGTTTAGTATGTACACCTTCATTTATTTGCTTTCATAAATG TTCCTACTGATGGTTTTGTTGGTTTTGTTTCACGTGTCTTCCTGATATTGACATATTTCCCTTCTGGGTT CTCTGTTTTGATCACTCATGTTGATTATTTTTTCCCCTGTAGCCGTGGTTTTTCCTTCTGTCCAATATTC ACTATTCCAATAGGAACAGTAATTTTAGATTTATTTTAATTGGCTCATGATGATGCTGTTATTCAATTAT TGTCTTTTTCAGTAAAGTATTCCATAACAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_024181
• Insert Size: 2382 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC033461, AAH33461
• RefSeq Size: 3616 bp
• RefSeq ORF: 2382 bp
• Locus ID: 66861
• UniProt ID: Q9DC23
• Cytogenetics: 2 C3
• Gene Summary: Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.[UniProtKB/Swiss-Prot Function]