Rnf130 (NM_021540) Mouse Untagged Clone

CAT#: MC205850

Rnf130 (untagged) - Mouse ring finger protein 130 (Rnf130), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf130
• Synonyms: 2510042A13Rik; G1rp; G1RZFP; GOLIATH; GP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC018199
  GCCCCGCCTCTCAGCACCGGCCCCGGAGCCCGGTCCGCGAGCGGCGGCGGCGGCCGGAGGGACCATGAGC GGCGCGGCGCGAGCGGGCCCGGCCCGGCTCGCGGCGCTGGCCCTGCTGACTTGCAGCCTGTGGCCGACGC GAGCGGACAACGCGAGCCAGGAGTACTACACGGCGCTCATCAACGTGACGGTGCAGGAGCCCGGCCGCGG CACCCCGCTCACGTTCCGCATCGACCGCGGGCGCTACGGGCTCGACTCACCCAAGGCCGAGGTCCGCGGC CAGGTGCTGGCACCGCTGCCCATCCACGGAGTTGCTGATCATCTGGGCTGTGATCCTCAAACACGGTTCT TTGTCCCTCCTAATATCAAACAGTGGATCGCCTTGTTACAAAGAGGAAATTGCACCTTTAAAGAGAAAAT ATCACGGGCTGCTTTTCACAATGCGGTTGCTGTAGTCATCTACAATAATAAATCCAAAGAGGAGCCGGTC ACCATGACTCATCCAGGCACGGGAGATATTATTGCTGTCATGATAACAGAATTGAGGGGTAAGGATATTT TGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCTGTTGGAACTCGAATGCCGCCAAAGAA CTTCAGCCGTGGCTCTCTAGTCTTCGTGTCAATCTCCTTTATTGTTTTGATGATTATTTCTTCAGCGTGG CTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCTCGGGACAGGAACCAGCGTCGTCTTGGAG ACGCAGCCAAGAAAGCCATCAGTAAATTGACAACCAGAACGGTGAAAAAGGGCGATAAGGAAACAGACCC AGACTTCGATCACTGTGCAGTCTGCATAGAAAGCTATAAGCAGAATGATGTTGTTCGGGTTCTCCCCTGC AAGCATGTTTTCCACAAATCCTGTGTGGATCCCTGGCTCAGTGAACACTGTACCTGTCCTATGTGCAAAC TTAATATTTTGAAAGCCCTAGGAATTGTGCCAAATCTGCCATGTACTGATAATGTAGCCTTTGATATGGA AAGGCTGACCAGAACGCAAGCGGTTAACCGGAGAGCAGCCCTTGGTGACCTTGCCGGTGACAGCTCCCTT GGCCTTGAGCCACTGAGAACTTCGGGGATATCGCCTCTTCCTCAAGATGGGGAGCTCACTCCTCGCACGG GTGAAATCAACATTGCAGTAACAAAAGAATGGTTTATTATTGCCAGTTTTGGCCTCCTCAGTGCTCTCAC ACTCTGCTACATGATCATCAGAGCCACAGCTAGCTTGAATGCTAATGAGGTAGAATGGTTTTGAAGAAGG AAAACACTGCTTTCTGACTGATTGCCTTGAAGGAAAAAAGAACCTATTTTTGTGCATCATTTACCAATCA TGCCACACAAGCGTTTATTTTTAGTACATTTTATTTTTTCATAAAATTGCTAATGCCAAAGCTTTGTATT AAAATAAATAAATAATAAAATAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_021540
• Insert Size: 1260 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018199, AAH18199
• RefSeq Size: 1506 bp
• RefSeq ORF: 1260 bp
• Locus ID: 59044
• UniProt ID: Q8VEM1
• Cytogenetics: 11 B1.3
• Gene Summary: Acts as an E3 ubiquitin-protein ligase (By similarity). May have a role during the programmed cell death of hematopoietic cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1.