Alkbh3 (NM_026944) Mouse Untagged Clone

CAT#: MC205847

Alkbh3 (untagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alkbh3
• Synonyms: 1700108H04Rik; 1810020C19Rik; Abh3; mABH3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC018196
  GACGGCTGATTAGGGTCGTGTCGGGACCTCTGCTCAGGGCCCAGCCAGGTAGCCATCCCTTGGTCTGAAT GGGGAATGAAATCTTCTGACATCTCAGAACAGAACGCTTTTTCTTCACCATGGAGGACAAGAGACAGCGA GCCCGAGTGCAGGGAGGCTGGGCTACACCTACCAAGAGCCAGTCTGCTACTCAGCCAGCTTCCCCTGCTA GGAGCCGTCTTTCCCAGACCGCGGGGCCAGCCTGGAGGAGCAAGGAACAGCAGCAATGTGACCGACAATT TGTGTTCAAAGAACCTCAGCTGGTTGTCCGTGCAGCCCCAGAACCCAGAGTGATTGACAGGGAGGGTGTG TATGAAATCAGCCTGTCACCTACTGGTGTGTCTAGGGTGTGCTTATATCCTGGCTTTGTGGACTTGAAGG AAGCTGACTGGATCTTGGAGCAGCTTTGTAAGGATGTCCCCTGGAAACAGAGGATGGGCATCAGAGAGGA TGTAACTTATCCGCAACCAAGACTTACAGCATGGTATGGAGAGCTTCCTTACACTTACTCGAGAATCACT ATGGAACCAAATCCTCACTGGCTTCCTGTGCTGTGGACTCTGAAGAGCCGCATTGAAGAGAACACCAGCC ACACCTTCAACTCCTTGCTGTGTAATTTTTACCGGGATGAGAAGGACAGTGTGGACTGGCACAGCGACGA TGAACCATCCCTGGGGAGCTGCCCCGTCATTGCTTCCCTCAGTTTTGGTGCCACTCGGACTTTTGAGATG AGGAAGAAGCCCCCACCTGAAGAAAATGGAGACTATACATATGTGGAGAGAGTGAAGATACCATTGGATC ACGGGACCTTGCTAATCATGGAAGGAGCCACACAAGCCGACTGGCAGCACCGAGTGCCCAAGGAATACCA CTCCAGACAACCGAGAGTAAACTTAACCTTTCGGACCGTTTATCCAGACCCAAGAGGAGCCCCTCGGTGA CACTTCTACAGAGTTACCACTGAGGGGAGGGCTATGCACCTAACAGCCCCTCCCCATCTCCCGCAAGCAC TTCCGTGATGTGGCTGTTCAGAAAGCGTGGTGTTTGCTTCTCAGGTTCTACTCCTCCTGAATGAGAGCTG CCAGCACGTCACCTTGTCAACATGTCTACTACAGGAACACTTACTTTGAGACAAATCTCTAAATCAAGGT TATCATTAAGCAAATTAAAAATTGCTCTGGCTGTACTCACAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_026944
• Insert Size: 861 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018196, AAH18196
• RefSeq Size: 1247 bp
• RefSeq ORF: 861 bp
• Locus ID: 69113
• UniProt ID: Q8K1E6
• Cytogenetics: 2 E1
• Gene Summary: Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (By similarity). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).[UniProtKB/Swiss-Prot Function]