Vps11 (NM_027889) Mouse Untagged Clone

CAT#: MC205803

Vps11 (untagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps11
• Synonyms: 1200011A11Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029004
  CACGTGATTTCGCTTCCGGAGGCGGGGGCCTTCGGCAAGATGGCGGCCTACCTGCAGTGGCGGCGTTTCG TTTTCTTCGAGAAGGAGCTGGTGAAGGAGCCGCTGGGTAATGATGGGGCTGCTCCCGGTGCCGCGCCTGT GTCGGGATCCGCTGCTTCCAAGTTCCTTTGCCTCCCTCCTGGCATCACTGTCTGCGACTCAGGCCGCGGG AGCCTGGTCTTTGGAGATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCTCTACAGCTTACAGGGTTCC AGGCCTACAAACTACGGGTGACACACTTATATCAGCTGAAGCAGCACAATATTCTGGCATCGGTTGGTGA GGATGAAGAGGGTATTAACCCTCTGGTAAAGATCTGGAACCTGGAAAAGAGAGACGGTGGCAATCCACTC TGCACCCGGATTTTCCCTGCCATCCCAGGGACAGAGCCAACTGTTGTGTCTTGTTTGACGGTCCATGAGA ATCTCAACTTTATGGCCATTGGGTTCACAGATGGCAGTGTCACCCTGAACAAGGGAGATATTACCCGGGA CCGGCACAGCAAGACGCAGATTTTGCACAAGGGCAACTATCCCGTTACTGGGCTGGCCTTTCGCCAAGCA GGAAAGACCACCCACTTGTTTGTCGTGACAACTGAAAATGTTCAGTCTTATATAGTTTCTGGAAAGGACT ACCCTCGTGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGTTCAGCCCTGAGCGACCCTTCTCAAGA CCTGCAGTTCATTGTTGCTGGGGATGAGTGTGTCTACCTTTACCAGCCTGATGAACGTGGACCCTGCTTT GCCTTTGAGGGCCATAAGCTCATCGTGCACTGGTTTAGAGGCTACCTTGTCATTGTCTCCCGTGACCGGA AAGTTTCTCCCAAGTCCGAGTTTACCAGCAGGGACTCACAGAACTCCGACAAGCAGATTCTCAACATCTA TGACCTGTGCAACAAGTTCATAGCCTACAGCGCTGGGTTTGAGGATATAGTGGATGTGCTGGCTGAGTGG GGCTCCTTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCATTGCAGGAGAAGGACACACAGACCAAAC TGGAGATGCTGTTTAAGAAGAATCTATTTGAGATGGCAATTAATCTGGCTAAGAGTCAACATTTGGACAG TGATGGGTTGGCTCAAATCTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCATGATGGGGCT GTGCAGCAGTATATCCGAACCATTGGAAAGTTGGAGCCATCCTATGTGATCCGGAAGTTTCTGGATGCCC AGCGCATCCACAACTTGACCGCCTACCTGCAAACCCTGCATCGGCAGTCTCTGGCTAATGCTGATCATAC CACCCTGCTTCTGAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAATTCATCAAGACAAAG AGTGAGAGTGAAGTCCACTTTGATGTAGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCCC ATGCCCTCTATCTGGCTGAGAATCATGCACATCATGAGTGGTACTTGAAGATCCAGCTAGAGGACATTAA GAACTATCAGGAAGCCCTTCGGTATATCGGGAAGCTGCCTTTCGAGCAAGCGGAGAGTAACATGAAACGC TATGGCAAGACCCTCATGCACCACATACCGGAGCAGACAACCCAGTTGCTGAAAGGCCTCTGCACTGACT ATCGACCCAGTCTGGAAGGCCGAGGCGATCGGGAGGCTCTGAGCTGCCGGGCCAGTTCTGAGGAGTTCAT CCCCATCTTTGCCAACAACCCACGAGAGCTGAAAGCCTTCCTAGAACACATGAGTGAGGTGCAGCCGGAC TCACCGCAGGGCATCTATGACACGCTCCTGGAGCTGCGGCTGCAGAACTGGGCCCATGAGAAAGATCCCC AGGCCAAGGAGAAACTTCATGCAGAGGCCATCTCCCTGCTGAAGAGTGGCCGATTCTGTGACGTGTTTGA CAAGGCATTGGTTCTGTGCCAGATGCACGACTTCCAGGATGGGGTCCTTTACCTCTATGAGCAGGGGAAG CTGTTCCAGCAGATCATGCATTACCACATGCAGCACGAGCAGTACCGGCAGGTGATTGCCGTGTGTGAGC GCCATGGAGAGCAGGAGCCCTCCCTGTGGGAACAGGCCCTCAGCTACTTCGCCCGCAAGGAGGAGGACTG CAAGGAGTATGTGGCGGCTGTGCTCAGGCACATTGAGAACAAGAGCCTCATGCCGCCTCTTCTAGTGGTG CAGACCCTGGCCCACAACTCCACAGCTACCCTGTCTATCATCCGAGATTACCTGGTCCAAAAACTGCAGA AACAGAGCCAGCAGATTGCACAGGATGAGCTCCGGGTGCGGCGATACAGAGAGGAGACCACTCGCATCCG CCAGGAAATCCAGGAGCTCAAGGCGAGTCCAAAGATTTTCCAGAAGACCAAGTGTAGCATCTGTAACAGT GCCTTGGAGCTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTACT CAGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAGAACCGCAAGGTGATGGATATGATCCGGGCCCA GGAACAGAAACGAGATCTCCACGATCAGTTCCAACATCAGCTCAAGTGCTCGAATGACAGCTTCTCTGTG ATTGCGGACTACTTTGGCCGAGGGGTTTTCAACAAGTTGACTCTGCTGACCGACCCTCCTACAGCCAGAC TGACCCCCAGCCTGGAGGCTGGACTGCAGCGGGACCTGCTGATGCACTCCAGGAGGGGAACTTAGTTAGG CAGCTCCTGGGGAAGCCTATATCCCTGGGCCTCTGAAGCACAGACGTAGGCCTGAGGAAGAGATGGTAGC AGACATGACCACAGTTCCGGAGCCTCAGGACTCAAGAAGGGACTCTGCTTTTCCTGCCTCCTCCTTTTGC CAGCCTGCTGTCCCTCCTGCTGACCAGCAGTATAGATTCTTCTACACCAGTGAGGAAGGCCACCTCAGCA ACCTCTCTGGACAATAGCTGCTTTATTCTCTACTCAAGGGCACCAGGCTGTGCTTGGGTCCTTGCTCCCA GAGCCTATAAATAAAAGTATAATAGTATGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027889
• Insert Size: 2826 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029004, AAH29004
• RefSeq Size: 3194 bp
• RefSeq ORF: 2826 bp
• Locus ID: 71732
• UniProt ID: Q91W86
• Cytogenetics: 9 A5.2
• Gene Summary: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.