Sec63 (NM_153055) Mouse Untagged Clone

CAT#: MC205785

Sec63 (untagged) - Mouse SEC63-like (S. cerevisiae) (Sec63), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sec63
• Synonyms: 5730478J10Rik; AI649014; AW319215
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019366
  CGGACGCGTGGGCGCGGCGGAGGAGGCGGAGCCATGGCGGGCCAGCAGTTCCAGTACGATGACAGCGGGA ACACCTTCTTCTACTTCCTCACCTCCTTCGTGGGGCTCATCGTGATCCCCGCCACCTACTACCTCTGGCC ACGAGACCAGAACGCGGAGCAAATTCGATTAAAGAATATCAGAAAAGTATATGGAAGATGTATGTGGTAT CGTTTACGGTTATTAAAACCCCAGCCAAATATTATTCCTACAGTAAAGAAAATAGTTCTGCTTGCAGGAT GGGCACTGTTCTTATTTCTTGCATACAAAGTTTCCAAAACAGACCGAGAATATCAAGAGTACAATCCTTA TGAAGTACTAAATTTGGATCCTGGAGCAACAGTGGCAGAAATTAAGAAACAGTATCGCTTGTTGTCACTT AAATATCATCCAGATAAAGGAGGTGATGAAGTCATGTTCATGAGGATAGCAAAAGCATATGCAGCTTTAA CAGATGAAGAGTCTCGGAAAAATTGGGAAGAATTTGGAAATCCAGATGGGCCTCAAGCTACAAGCTTTGG AATTGCTTTGCCAGCTTGGATAGTTGACCAGAAAAATTCAATTCTGGTTTTACTTGTGTATGGATTGGCT TTTATGGTTATTCTTCCTGTTGTGGTGGGCTCTTGGTGGTATCGGTCAATACGATATAGTGGAGACCAGA TTCTAATACGTACAACACAGATTTATACATACTTTGTTTATAAAACTCGAAATATGGATATGAAACGTCT CATCATGGTTTTAGCTGGAGCTTCTGAATTTGACCCTCAGTATAATAAAGATTCCACAAGCAAGCCAACA GATAATATTTTAATACCACAGCTAATCAGGGAAATTGGCAGCATAAATTTAAAGAAGAATGAACCTCCAC TCACCTGTCCATACAGCCTGAAGGCCAGAGTTCTTTTACTGTCTCATCTTGCTAGAATGAAAATTCCTGA GACACTGGAAGAAGATCAACAGTTCATGCTGAAAAAATGTCCTGCTCTGCTTCAAGAAATGGTTAATGTA ATCTGCCAGCTAATAATAATGGCCCGGAGCCGTGAAGAAAGGGAGTTTCGTGCTCCAACTTTGGCATCCT TAGAAAACTGTATGAAGCTTTCCCAGATGGCTGTTCAGGGGCTCCAGCAGTTTAAGTCTCCCCTTCTGCA GCTCCCTCATATTGAAGAGGACAATCTTAGGAGAGTTTCGAATCATAAAAAGTACAAAATTAAAACTATC CAGGATTTGGTTAGTTTAAAAGAATCAGATCGTCATAGTCTGCTACACTTCCTTGAAGATGAAAAGTATG AAGAGGTCATGGCGGTCCTTGGGAGTTTTCCGTATGTGACCATGGACATAAAATCACAGGTCTTGGATGA TGAAGATAGCAACAACATCACAGTAGGATCCCTAGTTACAGTGTTGGTTAAGTTGACGAGGCAGACCATG GCTGAAGTGTTTGAAAAGGAGCAGTCCATCTGCGCCGCTGAGGAGCAGCCCACAGAAGACGGGCAGAGTG ATGCCAACAAGATCAAGGCAAAAGGAGGATGGCAGCAGAAGAACAAAGGACCCAAGAAAATGCCCAAATC CAAAAAAAAGAAGCCTTTAAAAAAGAAACCCACAACTGTGCCCTTACCTCAGGCAAAGCAGCAGAAGCAA AAGCAGGCAAATGGAGTTGTTGGGAGTGAAGCTGCAATAAAGGAGGAAGAAGACGACATTTCTGACAAGG GCAGTGATTCTGAAGAGGAAGAAACCAATAGAGATTCTCAGAGTGAGAAAGAAGATGGTAGTGACAGGGA GTCTGATAGAGAGCAAGATGAGAAACAAAGCAAAGATGATGAAGCAGAGTGGCAAGAGTTACAACAGAGT ATACAGAGGAAAGAGAGAGCTCTTTTGGAGACCAAGTCAAAGATAACACACCCTGTCTATAGTCTCTACT TTCCTGAGGAAAAACAAGAGTGGTGGTGGCTTTATATTGCAGATAGGAAAGAACAAACATTAATATCCAT GCCATATCATGTGTGCACACTGAAGGATACAGAAGAGGTAGAACTGAAGTTTCCCGCACCCGGCAAGCCT GGAAATTATCAGTATACGGTGTTCCTGAGATCAGACTCCTATATGGGCTTGGATCAGATTAAACCACTGA AGTTGGAAGTTCATGAGGCCAAGCCTGTGCCAGAAAACCACCCACAGTGGGACACAGCGATAGAAGGTGA CGAAGACCAGGAAGACAGTGAAGGATTTGAAGACAGCTTTGAGGAAGAGGAGGAGGAAGAGGAAGGTGGA GACTAACCCGAGTGAGTGGACTACAGCGTTTGCACATCCTTGCAAACATTGCTGCTCTGTGAGAGGTCAT AAAGCAGCAGCAGTGAGGGCTGATATGGAGGGAAGGGTTGCTGACTTTACTAGAAACGGATGCAAAAAAG AGGCCGCTGGTGCCAGGGTACAATCTGAAAAGGGTTTTTAAAGCCTTTCAGGTATGGGGTGGTGCGTTTA TCTCATCTGCAAATGATAATAAATCCTTTGATGTTCCAACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_153055
• Insert Size: 2283 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019366, AAH19366
• RefSeq Size: 2610 bp
• RefSeq ORF: 2283 bp
• Locus ID: 140740
• UniProt ID: Q8VHE0
• Cytogenetics: 10 22.89 cM
• Gene Summary: Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed:22375059). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (By similarity). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (PubMed:21685914).[UniProtKB/Swiss-Prot Function]