Chaf1b (NM_028083) Mouse Untagged Clone

CAT#: MC205728

Chaf1b (untagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chaf1b
• Synonyms: 2600017H24Rik; C76145; CAF-I p60; CAF-Ip60; CAF1; CAF1A; CAF1P60; MPHOSPH7
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013532
  CGGAAACTCTGGAGGATGAAAGTGATCACGTGTGAAATCGCCTGGCACAACAAGGAGCCAGTGTACAGCC TGGACTTCCAGCATGGGGCAACCTGGAAGATCCACAGGCTGGCATCCGCGGGGGTGGACACCGCCGTCAG GATCTGGAAGTTGGAGAGGGGGCCAGATGGGAAAGCCATTGTGGAATTTCTCTCCAATCTTGCTCGACAC ACCAAAGCTGTCAATGTTGTACGCTTTTCTCCAACTGGGGAGATTTTAGCATCTGGAGGAGATGATGCCG TCATTCTGTTGTGGAAGATGAATGACAGCAAGGAGCCGGAGCAGATCGCCTTTCAGGATGAGGAGGAGGC TCAGCTGAATAAGGAGAACTGGACTGTGGTAAAGACCCTGAGGGGCCACTTAGAAGATGTGTATGATATT TGCTGGGCAACCGATGGGAATTTGATGACCTCTGCCTCTGTGGATAACACAGTTATCATATGGGATGTCA GTAAAGGGCAGAAAATTTCCATTTTCAACGAGCATAAAAGTTATGTGCAAGGAGTCACCTGGGATCCTTT GGGTCAATATATTGCGACCCTGAGCTGTGACAGGGTGCTGCGGATCTATAATACCCAGAAAAAGCGTGTG GCTTTCAACATTTCAAAGATGCTCTCTGGACAGGGGCCTGAAGGAGAGGCAAGAAGTTTCCGGATGTTTC ACGACGACAGCATGAAGTCGTTCTTCCGAAGGCTCAGCTTTACTCCAGATGGATCTTTGCTCCTCACTCC AGCCGGATGTATGGAATCTGGTGAGAATGTGACAAACACCACTTATGTGTTCTCCAGAAAACACCTGAAA AGGCCAATCGCCCATCTTCCGTGCCCTGGGAAAGCGACTCTTGCTGTCCGCTGTTGTCCTGTCTACTTTG AATTGAGGCCAGTGGCAGAAACAGAGAAAGCATCAGAGGAGCCCAGCCCCGAGCTGGTGAACCTGCCCTA CCGTATGGTGTTCGCGGTGGCTTCCGAGGACTCTGTGTTGCTCTATGACACTCAACAGTCTTTCCCATTT GGCTATGTGTCTAACATCCATTACCATACCCTGAGTGACATTTCCTGGTCCAGCGATGGGGCCTTCTTGG CCATTTCCTCCACGGATGGCTATTGCACATTTGTGACCTTCGAGAAAGGTGAACTTGGCATACCTTTGAA AGAGAAGCCAGTGCTGAGCATAAGGACTCCCGACACAGCCAAGAAAGCCAAGAACCAGACACACCAGGGA TCTTCGCCGGGATCCAGATCAGTCGAAGGGACCCCCAGCAACAGAACCCAAGACCCCAGCAGTCCCTGTA CCACTCCTTCACCAACCACACAGTCTCCAGCTCCGTCGGCCATCAAGGACAGTCCCTCAGCCATCCCGGC TGGCAAAAGCCCCTTGCCACAACCTTCTGAAGAGAAGACCCTGCAACCTGCTGGGCAAAACATGAAAGCC CCCCAACCCCGCAGGGTCACCCTGAACACGCTGCAGACTTGGGGCAAGACAGCCCCCCGGAGAATAAATT TAACACCATTGAAAACAGACACTGTGCCAAATCCACAGCCCAATAGCGGGACCGCCCCCTCCACAGAAGA GGTCCAGCCAGAGGCTCCTGGGGAGCCACCGGAGGAGCCTCCTGAACTGAAGAGACCCAGGCTTGAGGAA CGTGAAGGAGATGCCCAAAACCTGGCCCCTGATGACTCTTCCAAGACTGTCTGAGCCTGCTATGCACCTG CGTGCAGAGGAGGCAAGGCCAAAGCTCGAAGGCTGGTGATGGTGGCCAAATAAACACTCATGAATTGGTT CACATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028083
• Insert Size: 1719 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013532, AAH13532
• RefSeq Size: 1893 bp
• RefSeq ORF: 1719 bp
• Locus ID: 110749
• UniProt ID: Q9D0N7
• Cytogenetics: 16 54.96 cM
• Gene Summary: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).[UniProtKB/Swiss-Prot Function]