Clk2 (NM_007712) Mouse Untagged Clone

CAT#: MC205713

Clk2 (untagged) - Mouse CDC-like kinase 2 (Clk2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clk2
• Synonyms: AU041688
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015080
  CCACGCGTCCGGCGGCGGCGGAGCGGAGAGGCCAGAGCCGGAGACCGAGCTGGGATCGGGCCCCGGGCGG GGGCGGTGCGAGAGGCGCCGAGCGGATCCTGGGGGCGGGGACGGAGCCCGGGCGGGCGGGACTGGAGCAG AGCCCGGGAACGGGGCGGGAGGTCCCAGGGCCCCGGGTTGGGGGGTGGAACCGCACTTCGTCGCCGCGGG GGTGCCGGGACTCCGGCCGCAGTGCCGCCGCCATCACGGACTTCCTGTGGGACAAGCGCACGGGCCTCGC CGCCAGAACGATGCCCCATCCCCGAAGGTACCATTCCTCAGAGCGAGGTAGCCGGGGGAGTTACCACGAA CACTATCGGAGCCGAAAGCATAAGCGAAGAAGAAGTCGCTCCTGGTCAAGTAGCAGTGACCGGACAAGGC GGCGGCGGAGGGAGGACAGCTACCACGTTCGGTCCCGAAGCTATGATGACCATTCGTCCGATCGGCGGCT GTACGATCGGCGGTACTGTGGCAGCTACAGGCGCAATGACTACAGCCGGGACAGAGGGGAGGCTTACTAC GACACAGACTTTCGGCAGTCCTATGAATACCATCGAGAGAACAGCAGTTACCGAAGCCAGCGCAGCAGCC GAAGGAAACACAGAAGGCGGAGGAGACGGAGCCGGACATTCAGCCGCTCATCTTCACACAGCAGCCGGAG AGCCAAGAGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAGCGA TATGAAATTGTAAGCACCTTAGGAGAAGGGACCTTCGGCCGAGTTGTGCAGTGTGTGGACCATCGCAGGG GCGGAACACGAGTTGCCCTGAAGATCATTAAGAATGTGGAAAAGTACAAGGAAGCAGCCCGACTAGAAAT CAACGTGCTGGAGAAAATCAATGAGAAAGATCCTGACAACAAGAACCTCTGTGTCCAGATGTTTGACTGG TTTGACTACCATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAG ACAACAACTACCTGCCCTACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTCTGCCAGGCCGTCAA GTTCCTCCATGATAACAAGTTGACACATACGGACCTCAAACCTGAAAATATTCTGTTTGTGAATTCAGAC TACGAACTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTAAAGAGCACAGCCGTGCGGGTGG TGGACTTCGGCAGTGCCACCTTTGACCATGAACACCATAGCACCATTGTCTCCACTCGCCATTACCGAGC CCCCGAGGTCATCCTGGAGTTGGGCTGGTCACAGCCATGCGATGTATGGAGCATAGGCTGCATCATCTTT GAGTACTACGTTGGCTTCACCCTCTTCCAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGA TCCTGGGTCCTGTCCCTTCTCGGATGATCAGAAAGACAAGAAAACAGAAATATTTTTATCGGGGTCGCCT GGATTGGGATGAGAACACCTCAGCCGGGCGCTACGTTCGTGAGAACTGCAAACCTCTGCGGCGGTATCTG ACCTCAGAGGCAGAGGACCACCACCAGCTCTTCGATCTGATTGAAAATATGCTAGAGTATGAGCCTGCTA AGCGGCTGACCTTAGGTGAAGCCCTTCAGCATCCTTTCTTCGCCTGCCTTCGGACTGAGCCACCCAACAC CAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGACAATTAGGCTTTGGGCCCCCTGCTCCTTGTGTA GCAGTGGGTGTCCAATCCAGGACACTGGTGCTTTTTTATACAAAAGAACAGAGCCAGAACTTGCCCTCTC TCCAGCCCCTGGCTCCTTTGTACCTGTGAATATGTGGAATAGTGTAAATATGACAGAACTTGTACCTATC GATTCAACCCCTGACATGTATATACTACTATTTACACTCTCCCAACCTCATCTGCCCCTCATGTGGAGGT GGATGGGGGGCAGAATGACGTAACCAGGTGGTGTCTACCCCATGTTTTATAAGGGATTTTGTACAGTCTT TGTGAAATAAAATGACATGCTTCATCTGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007712
• Insert Size: 1497 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015080, AAH15080
• RefSeq Size: 2143 bp
• RefSeq ORF: 1497 bp
• Locus ID: 12748
• UniProt ID: O35491
• Cytogenetics: 3 F1
• Gene Summary: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).