Rap1gds1 (NM_145544) Mouse Untagged Clone

CAT#: MC205710

Rap1gds1 (untagged) - Mouse RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rap1gds1
• Synonyms: BC011279; GDS1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011279
  GCCCTGCCGGTCCTCACTCTGTGGGAGGAACATGGATAACCTCAGTGACACTTTAAAGAAGCTGAAGATA ACAGCTGCTGACAGGACAGAGGGCAGTCTGGAAGGATGCTTGGATTGTCTGCTTCAAGCCTTGGCTCAGA ATAATGCAGAAACAAGTGAAAAAATACAAGGAAGTGGAATTCTTCAGTTGTTTGCAAATCTGTTGACGCC ACAGGCCTCCTGCACAGCCAAAGTAGCCAACATCATAGCAGAAGTAGCCAAAAATGAATTTATGCGAATC CCATGTGTAGATGCTGGATTGATTTCACCACTGGTACAGCTGTTAAATAGCAAAGACCAGGAAGTGCTGC TTCAAACGGGCAGGGCATTAGGAAACATATGCTACGATAGCCATTCCCTTCAGGCCCAGCTTATTAGCAT GGGTGTTATCCCGACCTTAGTGAAGCTACTGGGCATTCACTGCCACAATGCAGCACTCACCGAGATGTGT CTCGTTGCGTTCGGGAATTTAGCAGAACTTGAGTCAAGTAAAGAACAGTTTGCTAGCACAAACATCGCTG AAGAGCTGGTAAAACTGTTCAAGAAACAGATAGAACACGATAAAAGAGAGATGATTTTTGAAGTCCTAGC GCCGTTGGCAGAGCATGATGCTATTAAGCTGCAGCTGGTTGAAGCAGGTTTGGTAGAGTGTCTCCTAGAG ATCGTCCAACAGAAGGTGGATAGCAACAAAGAGGACGACGTTGCCGAGCTTAAAACTGCTTCTGACCTCA TGGTTTTATTACTGCTTGGAGATGAGTCCATGCAGAAACTTTTTGAAGGAGGAAAAGGCAGTGTGTTCCA AAGGGTGCTCTCCTGGATTCCATCGAATAACCACCAGCTGCAGCTGGCTGGGGCATTAGCCATTGCAAAT TTTGCCAGGAACGATGGAAATTGTATTCATATGGTAGACAATGGGATTGTAGAAAAACTCATGGATTTGC TGGACAGACACGTAGAAGACGGGAACGTGACGGTGCAGCACGCCGCACTAAGTGCCCTCAGGAATCTAGC CATCCCAGTTGTAAATAAAGCAAAGATGTTATCAGCTGGTGTCACGGAGACGGTTTTGAAATTCCTTAAA TCTGAAATGCCCCCAGTTCAGTTCAAACTCCTGGGAACGCTAAGAATGTTAATAGATGCACAAGCAGAAG CTGCTGAGCAACTGGGAAAGAATGCTAAACTGGTGGAGCGTTTGGTTGAATGGTGTGAAGCCAAAGATCA TGCCGGCGTGATGGGCGAATCAAACAGACTGCTCTCTGCCCTCATCCGACACAGTAAATCAAAAGATGTA ATTAAAACCATTGTACAGAGTGGTGGCATCAAGCACCTGGTTACCATGGCAACTAGTGAACATGTAATCA TGCAGAATGAAGCTCTTGTCGCCCTGGCACTGATAGCAGCCTTGGAACTGGGTCCCGCTGAGAAGGATCT CGCAAGTGCTCAGCTTGTTCAGATCTTACACCGACTGCTAGCAGATGAGAGAAGTGCCCCGGAGATCAAG TACAATTCCATGGTCCTGATCTGCGCTCTCATGGGATCCGAATCTCTATACAAGGAAGTACAGGATTTGG CCTTTCTAGATGTTGTCTCCAAACTTCGAAGTCATGAGAACAAAAGTGTTGCCCAACAGGCCTCGCTTAC AGAGCAGAGACTTACAGTGGAAAGCTGAGAACTGGCCCTGCTGGCACCAGGCTCATCCCAGCTCCGCCCA CTCCACTGTTCTCCATCCAGCTGCTTCTTCAACTCCATTCTCTACTACATCACTTGTGCATGCGTGTAAT GTTCCAGTACCAATGGAAGAACTGCTATAGGTACCTGCCTAATAAGATTTCTAAACCCATAGTTAATGTG AACATGACTGTCAGAACAAGTCTCCACCCATAACTGTTAAATCCTATACTCCTGATGCTTGAGCTACATT AAGTAGAATGTGCATGTTGTAGTCCTATGATGATGTAAACTTGGTACTCCATGCTGACTTGCTCCTCACA TGCAGTAAGCTACATAATAATGTACTGGTAAACTAGAAACAAAGAATGCAGCAGGACCTGTCTAGCTTAT TTAAAGATGAGACTGAATCGTCAAGGTAGCACCACGTATTCTGGTTTTGTTTTGTTTTGGTGCTGGGGAA GCACAGCGATCAAAAAGCCTGTATGGCTGCTCACTCATTAATCTTCTGCCAACGTCAGGCCTGTTCCACC TAAACTTGAATAGGTCCAATATTCCTTCCTTCCCTACGGTCTCTCCTTTTTCTGAACTATATAGGCAGTT TGCCAAGTAACACAGCAAACCTTCCTGAGGCTCCTGATTCCATCTTTAATCGACAGTGGTACAGGGGCTC AGTGCTGAAACGTGGATTTTAATGTGAGCGGAGCGTAGGAAGGAGTTAAAGAAACCCTGGCATACTGAAC CACTTTGGATCTTGCCTGTACTTTAGATCCGATTTGTGTGTGGTTACATCTAGAACTTCCATCAGCTAGC CAGCATTGTCAACATGTCTCTAAAGCGTCCTTGGTGTTTGTTCGGTGAAGGAAACTGAGGCGGCTTGTTT GGTGGAGAATGGACTTCCCCCCCCCGCCCCGTTTTCATCTTTTAGGGTGAAAGCAGGGAGGTTCATCTTT CATGCTCCCTTTCAATGTGCTAATTCTAATTGGACATAAGAGGAAACCTGAAGTAAGCTTGTCTAAAGAG AGGAAGGCCGGTTTTAAAAGCCATAGTAGCCAGTCCAGTACATTCTGCAGATGGCCTCATAGCAGTCATT GTTAGAGTTCTTCACCTCCACTTTCCCACTGAGAACCACAATAATCTCCCAGGCTGCAAGCCAGGACTCG CCGCAAAGACTCTTCTTGGCATAATGTAGTGTGCTCGTCACTGCCACTAACCACCACCAAGTGAGTTCTT AGAGCTTTGACACACAGTCTTTATAAATTAATCGGGATTCCCTGTGACCCGCCTGCCTCCTCACACCCAA GGTTGAGTCCTGCTGATGTCCGAGGGAGGCTGAGCTCCGTTACAGGAGTGGAGGGCAGTGAGGGGCTGCT GAGAGGGACCAGCTCCTCCCTCCCCAGCTCCTCCCTCCCCCAACAGGAGATACCTGTATCTCTGCGCTCT CTAAGTCGGACTTTGCTTTTCACACTTTTAAATTCAAGAAAGATTTACTTCATGTAAGTAGAAACCTGAC TGACAGTTCCCTCTGATGTCCAAATAAGGGAACTGTGGTGAGCGCCTGTGCTTTATTGTCTTTCTCCTGG GCTGGATAAGTGGACTAATGTTGCATAATACTGTCCATATTAATAACTTAATAAATAAATAGTTTATGAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145544
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011279, AAH11279
• RefSeq Size: 3373 bp
• RefSeq ORF: 1677 bp
• Locus ID: 229877
• UniProt ID: E9Q6Q4
• Cytogenetics: 3 H1
• Gene Summary: Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake.[UniProtKB/Swiss-Prot Function]