Memo1 (NM_133771) Mouse Untagged Clone

CAT#: MC205701

Memo1 (untagged) - Mouse mediator of cell motility 1 (Memo1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Memo1
• Synonyms: 0610016J10Rik; D930048L02Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013819
  GGCCAGTCGCCGCCGGGCCGTTGCTCCGCGGAGCTTCCCGGATCCGAGCCCAGACGGCGTCGGCCGGGGC AGCCTGGCCGCCGCCTCCTCATTCATCCTCGTGCACCATAGGCGGCACAGGCACCAAGATGTCCAACCGA GTGGTCTGCCGGGAAGCCAGCCACGCCGGGAGCTGGTACACCGCCTCAGGACCTCAGCTGAACGCTCAGC TGGAAGGTTGGCTTTCACAAGTACAGTCTACGAAAAGACCTGCTAGAGCCATTATTGCACCCCATGCGGG ATACACATACTGTGGGTCCTGTGCTGCCCATGCTTACAAACAAGTGGATCCATCTGTTACCCGGAGAATT TTCATCCTTGGGCCTTCCCATCATGTGCCCCTGTCTCGATGTGCACTCTCCAGTGTGGATATATATAGGA CTCCTCTGTATGATCTTCGTATTGACCAAAAGATTTACGGAGAGCTATGGAAGACAGGAATGTTTGAACG AATGTCTCTGCAGACAGATGAAGATGAACACAGTATTGAAATGCATTTGCCTTATACAGCTAAAGCCATG GAAAGCCATAAAGATGAGTTTACCATTATTCCTGTACTGGTTGGAGCTCTGAGTGAGTCAAAAGAACAGG AATTCGGAAAACTCTTCAGTAAATATCTAGCGGATCCTAGTAATCTCTTTGTGGTTTCTTCTGATTTCTG CCACTGGGGTCAAAGGTTCCGTTACAGTTACTATGATGAATCCCAGGGGGAGATTTATAGATCCATTGAA CATCTAGATAAAATGGGTATGAGTATTATAGAACAATTAGATCCTGTATCTTTTAGCAATTATTTGAAGA AATATCATAATACTATATGTGGAAGACATCCCATTGGGGTGTTATTAAATGCTATCACAGAGCTTCAGAA GAATGGAATGAATATGAGCTTTTCCTTTTTGAATTATGCCCAGTCGAGCCAGTGTAGAAGCTGGCAAGAC AGTTCAGTCAGTTACGCAGCTGGAGCACTCACGGTCCACTGAAGCTCCGATCCTCAGGGACACCACCTGC ACAGACTCGTACTCTGTCCAGGGTCCAGCCTAGCCTTTACCAAGATGTTGGTTCTGGTGTGGGGGGATTC TGAAACCTCAGACTAATAGAACTCTCTTCTTCTTTTCTAGTAGGTGTAGTCCTCCTTAATTTCAACTCAT TTTAAAAATGCTTTCTAGTTTGAGGCAGTGGAAGGAAGGCTGGAATCAAAATATTTGCCCAAATTGGCAC ACAGTGACTTGTAAAGCATTTAGCATATCTGAAATTTGCACTCTTTGCAGACTCATGCACATATATTCCG CTTTCAGAATAGTTTTGCAAATTGTACACAGACAAAGGGTGGAAGCTTTTTAATAAAAGAAACTGCATTT ATAAATGATCTGTATTAGAATATAAGAAATCTCCAGTTATAGACAATTACTACCCATGTTGTACAACAGA TACCTTCTATTTTAGTTGCTAATAAAGGGCTACACAACTCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133771
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013819, AAH13819
• RefSeq Size: 1525 bp
• RefSeq ORF: 894 bp
• Locus ID: 76890
• UniProt ID: Q91VH6
• Cytogenetics: 17 E2
• Gene Summary: May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).[UniProtKB/Swiss-Prot Function]