Ctss (NM_021281) Mouse Untagged Clone

CAT#: MC205662

Ctss (untagged) - Mouse cathepsin S (Ctss), (10ug)


  "NM_021281" in other vectors (4)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ctss
Synonyms Cats
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC002125
GAGCACCACACTTCAGGATGACCATGAGGGCTCCTGGCCACGCTGCCATAAGATGGCTGTTTTGGATGCC CCTGGTGTGCTCTGTGGCAATGGAGCAACTGCAGAGAGACCCTACCCTGGACTACCACTGGGATCTCTGG AAGAAAACCCATGAGAAAGAATACAAGGATAAGAATGAAGAAGAAGTACGGCGTCTCATCTGGGAAAAGA ATCTGAAGTTTATAATGATTCACAATCTCGAATATTCTATGGGAATGCATACCTACCAAGTGGGCATGAA CGATATGGGAGACATGACCAATGAAGAAATCTTGTGTCGGATGGGTGCTCTGAGAATTCCCCGACAGTCT CCGAAGACTGTCACTTTCAGGTCATACTCTAATCGGACATTGCCTGACACTGTGGACTGGAGAGAGAAGG GCTGCGTCACTGAGGTGAAATACCAGGGTTCTTGTGGTGCCTGTTGGGCTTTCAGTGCTGTGGGGGCCCT TGAAGGGCAGCTGAAGCTGAAAACGGGGAAGCTGATATCCCTCAGTGCTCAGAACCTGGTGGACTGCTCA AATGAAGAAAAGTATGGGAATAAAGGCTGTGGAGGCGGCTACATGACCGAAGCTTTCCAGTACATCATTG ATAATGGGGGCATAGAGGCAGACGCTTCCTATCCCTACAAAGCCATGGATGAAAAGTGTCACTATAACTC AAAAAATCGAGCTGCTACGTGTTCAAGGTACATTCAGCTCCCCTTTGGTGACGAGGATGCCCTGAAAGAA GCAGTGGCCACTAAAGGGCCTGTCTCTGTGGGCATCGACGCCAGCCATTCCTCCTTCTTCTTCTACAAAA GCGGTGTCTATGACGACCCCTCCTGTACGGGCAATGTGAATCATGGTGTTCTTGTGGTTGGCTATGGGAC TCTTGATGGCAAAGATTACTGGCTTGTGAAAAACAGTTGGGGCCTTAACTTTGGTGATCAAGGATACATT CGGATGGCAAGAAATAATAAAAATCACTGCGGAATTGCTAGTGATTGCTCTTACCCAGAAATCTAAACCA TTTCTTCTTTTTCTAACAAGTCATAAAGATGGAATATACTCTAACTTAATTTGCCTGCTTTATCCAGAGG AACCAAGTGCGTCGTGATCAATGTGCAGTTACTGTACTAACTAGGTTACAGCTATTTTTACACCCTGTTG TCTAATGTTTTCTAATAACCGTTTGTAACATGATAGCCCCAAAATGCTTAATAAAATCTGTCATTTCAAA AAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_021281
Insert Size 1023 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC002125, AAH02125
RefSeq Size 1279 bp
RefSeq ORF 1023 bp
Locus ID 13040
UniProt ID O70370
Cytogenetics 3 40.74 cM
Gene Summary This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1.

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