Dlst (NM_030225) Mouse Untagged Clone

CAT#: MC205630

Dlst (untagged) - Mouse dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlst
• Synonyms: 1600017E01Rik; 4632413C10Rik; 4930529O08Rik; DLTS
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006702
  GATGCTGTCCCGGTCACGCTGCGTGTCCCGGGCGTTCAGCCGCTCGCTTTCTGCCTTCCAGAAGGGGAAC TGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGTCGGGGACCAGGTTACCCTGACAACAGGA AGATGGTCATTAACAGTGGTAGCGTCTTCAGGGTTCGCTTCTTCCAAACCACGGCTGTGTGCAAGAATGA TGTGATAACAGTCCAAACCCCAGCGTTTGCAGAGTCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCT GTTGGAGATGCGGTTGCAGAAGATGAAGTGGTGTGTGAGATTGAGACAGACAAGACTTCTGTGCAGGTTC CGTCGCCAGCAAATGGCATCATTGAAGCTCTTTTGGTACCCGATGGGGGCAAAGTTGAAGGAGGAACTCC TCTGTTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAACCAGCTGAAACTCCTGCTCCAGCC CACAAAGCAGAGCCCGCAGCACCGGCGGCCCCTCCTCCCCCCGCAGCACCTGTGCTCACTCAGATGCCAC CAGTGCCCTCACCCTCACAGCCTCCTTCTAGCAAACCAGTGTCTGCAATAAAACCCACTGCTGCCCCTCC ACTGGCTGAGGCAGGAGCTGCTAAAGGTCTGCGTTCAGAACATCGGGAGAAAATGAACAGGATGCGGCAG CGCATCGCCCAGCGTCTGAAGGAAGCCCAGAACACGTGTGCAATGCTGACGACTTTCAATGAGGTTGACA TGAGTAACATACAAGAGATGAGAGCTCGGCACAAGGATGCTTTCCTGAAAAAACATAACCTCAAATTAGG CTTCATGTCAGCATTTGTGAAGGCCTCGGCGTTCGCCTTGCAGGAGCAGCCTGTTGTAAATGCAGTGATT GATGATGCAACCAAGGAAGTGGTGTATAGAGATTATATTGACATCAGTGTTGCAGTTGCTACCCCGAGGG GTCTCGTGGTTCCTGTCATCAGGAATGTGGAAACTATGAACTATGCAGATATTGAACGGACCATTAATGA ACTAGGAGAGAAGGCCCGGAAGAATGAACTTGCCATTGAAGATATGGATGGTGGTACCTTCACCATCAGC AACGGAGGAGTTTTTGGCTCACTTTTTGGAACACCCATTATCAACCCCCCTCAATCTGCCATCCTGGGCA TGCACGCCATCTTTGACAGGCCTGTGGCTGTGGGAGGCAAGGTGGAAGTTCGACCTATGATGTATGTAGC CCTGACCTATGACCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCCGAAAAATCAAGGCAGCA GTAGAAGATCCAAGAGTCCTCCTCCTAGACCTTTAGGAGGAAGCCACACATCCTACCTATCGATCATGCA GGAACTGAAAACCACTCTTCTCCCTGTACCCTCATCAATCCCAGGTTAGCCTGATGACAGGCAGGCACAT ACTATTGGCCTCAAGCACGGAAGCCAGGGCACGATGTAACCAGCGTTCACAAGTCTGTTCTTAGTGTTCC CTCGAGGCTCTTTCTCTCTCCCTGAGACTCTTATCTTCTGCTATCTTGGACCTGAGAGAGAGTGAGAGCC TTAATGGATGCTCATTAATGTTCCTGCCTTTATTCCAGTCCTCTACAAAGGCAATTTTGCTTTTCTCCAG TAACAGTATGCTATAGGGATACCACCAGGGACCATGTGATTTGAGTTTCTATCTTAGGAAAGTGTTCTCC AGAGATGCCTAGGGGATGCTTTGCCTCCATCCCAGCTGTGCACACTCTGACTTGATGCCACCTGTGTGGA GGTATTGAACACAGGCAAAGAGGTGCTGCTTTGCTTCTTAAATGCACCATCATTCTGACCTGTCAGTGGC TTCAAGGTGCTCTACCTCTTCCAGAAAGCACAGGCTGGGGTATTTGGGGACAGGCAGGATTGTGCAGCAT CCCCTGAGCTTACCTGTTATAGTCACAGGTGGGAGTTGAACCCTGGCTTGCCCTCTCTGGCTCCATTGTA TGCTGGCACTGGGAAGATTCATTTTGGCATAATTACGTACAACTGTCGTCATACCAACCCTTTCCAGCTT CTAGGAATCGTTCTAGGACACTGTTGTCACTTAAAGCAGATTTTAGCACATCATGGCAGTGGGGCAGCCT GACGAAGGGACTGTTCTCACAAGATCAGGCTGAAGAGAGCTTTTAAATTCCAGGTGGTGTGAGCATGTTC TGGGAAGAGGCTGTGCTCTCCCTCCCTGGGATGTGGCCTGTGGGGAGCAAGGTCTTGTTGCCTCTGGTGC AGGGCACAGAGACAGGGGAGGGTGGACTGCGGTGGGAACCAGCCTCAGGAGTTAGGACAGCGACGAGATG ATGGTGTCATCTCTAAGATCACCCTCAGGTGCAGGTACAGTTGTTGGAATATTCTTGAAGCATGGTAAAA TGCTTAAGTGGTTTTGAGCGCTCCTAGGCCTCGTATCCTGCACTGAGCCTGAGCCTGCTGACAGTTGCCG TGGCCATTAGTGCTGAATGCCCTTTTTTCTTGTAATCCAAGACAAGCTGGGGGGAGGGGAAGAGGTCAGT ATTGAGTGCCTGCAGCATCTGCTACTACATCTACACCATTCAGAACTTGTAACTAAAATATTTAAAGAGA CTGATTTTGAATGAAAATTAAAGGGCAAATGTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_030225
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006702, AAH06702
• RefSeq Size: 2726 bp
• RefSeq ORF: 1365 bp
• Locus ID: 78920
• UniProt ID: Q9D2G2
• Cytogenetics: 12 D1
• Gene Summary: Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex (By similarity). The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) (By similarity). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity).[UniProtKB/Swiss-Prot Function]