Creb3l1 (NM_011957) Mouse Untagged Clone

CAT#: MC205492

Creb3l1 (untagged) - Mouse cAMP responsive element binding protein 3-like 1 (Creb3l1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Creb3l1
• Synonyms: Oasis
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016447
  GGAAAGCGCAGCCCGAAGGGGTCCCCTGGAACCCGGCGCGATGGACGCCGTCTTGGAACCTTTCCCGGCC GACAGGCTGTTCCCGGGATCCAGCTTCCTGGACTTGGGAGACCTGAATGAGTCGGATTTCCTCAACAATG CGCACTTCCCGGAGCACCTGGACCACTTTGTGGAGAACATGGAGGACTTCTCCAATGACCTGTTCAGCAG TTTCTTTGATGACCCTGTGCTGGACGAGAAGAGTGCTCTGCTGGACATGGAACTGGACTCCCCCGCTCCA GGCATCCAGGCTGAGCACAGCTACTCCCTGAGTGGGGATTCTGCACCCCAGAGCCCCCTTGTGCCTGTCA AGATGGAGGACACCACTCAAGATGTGGAACACGGAGCGTGGGCCCTGGGAAACAAGCTGTGCTCCATCAT GGTGAAGCAGGAGCAGAGCCCGGAGCTGCCTGTAGATCCCCTGGCTGCCTCCTCTGCCATGGCTGCTGCC GCCGCCATGGCCACCCCACCACTGCTGGGCCTCAGCCCCATGCCCCGGCTGCCCATCCCTCACCAGGCCC CAGGAGAAATGACTCAGCTGCCAGTGATCAAAGCAGAGCCCCCAGAAATGAGCCAGTTTCTCAAAGTGAC ACCAGAGGACCTCGTACAGATGCCTCCAACACCCCCCAGCAGCCATGGCAGTGACAGTGACGGCTCCCAG AGTCCCCGCTCTCTTCCCCCCTCCAGCCCTGTCCGGCCCATGGCCCGCTCCTCCACGGCCATTTCCACCT CTCCGCTCCTCACTGCCCCTCACAAACTGCAGGGGACATCAGGGCCACTGCTCTTGACAGAAGAGGAGAA GCGGACCTTGATCGCGGAGGGTTACCCTATCCCCACCAAGCTCCCCCTCACCAAGGCTGAGGAGAAGGCC TTGAAGAGAGTACGCAGGAAAATTAAGAACAAGATTTCTGCCCAGGAGAGCCGCCGCAAGAAGAAGGAGT ATGTGGAATGTCTAGAAAAGAAGGTGGAGACATATACATCAGAGAACAATGAGCTGTGGAAGAAAGTGGA AACCCTAGAGACTGCCAACAGGACCCTGCTCCAGCAGCTGCAGAAACTCCAGACTCTGGTCACCAGCAAG ATCTCCAGACCGTACAAGATGGCAGCCACGCAGACCGGCACCTGCCTCATGGTGGCAGCCTTGTGCTTCG TTCTGGTGCTGGGCTCCCTTGTGCCCTGCCTTCCTGCATTCTCTTCCGGCTCAATGACTGTGAAAGAAGA CCCTATCGCAGCTGACAGTGTCTATGCAGCCAGTCAGATGCCTTCCCGAAGCCTACTGTTCTACGATGAT GGGGCAGGCTCATGGGAAGATGGCCGAGGTGCTCTACTGCCTGTGGAGCCCCCAGAAGGCTGGGAGCTCA AACCCGGGGGTCCAGCAGAGCAGAGGCCCCAGGACCACCTCCGACATGACCGTGCAGACAGCATCCATGA GACCACCAAGTACTTGAGAGAGACCTGGCCAGAGGACACTGATGACAACGGCACCAGCCCCAACTTCTCC CACCCCAAGGAGTGGTTCCATGACAGGGATCTGGGCCCCAACACCACCATCAAACTCTCCTAGGCCACTC CAAGACCCAGGACACAGGACGGACACCCTGGCACCCAGAAGAGGCGTTCTCTTGCTCGATGACCCAGATC CAGCTCATACCCCTGCCCCCCCGGGTCCCTGTAGTAGCTGGGGACGCTCTATGTCCCCAGACACTTGGAC TGCTCCCCTGGGCTGACCACTCCGTTCCCACCTTTCCCTCCTACCACTATCCGTCCTCCTCCGATAAACC ACTCACTGGGCTACCCGTTTCCTTCCCATAGTGACCAACGCAACCACTGTTCCTGGCCCCCTCATACACA AACACACAAGCAGACATACACACACAAACACACACCCCCATCTCCTACTGTACAGAGACCAAGAACAGAA ATCGTTTGTAAATAATGAACCTTATTTTTTATTATTGTCAATCCCCTAAGATATTGTATTTTACAAATCT CCCTCCCGTCACCTCTCCCTTATTTTGTATTTTATGAAGTTAGTGCGGGCTTTGCTACCCCTTGGCCTGG GGAAGAGGGACACCCCACCCTCACCAGGCCTCTCCCTGCTGCTGCCCAAGCTGCTGGGCCTTTTTAATTT GCCAAACTGCTCCTCCACCAGCTCAGCACATGCTTTAAGAAGGCAAAATTAAAAAAAAAAAAAAGATTCA GCATCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011957
• Insert Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016447, AAH16447
• RefSeq Size: 2260 bp
• RefSeq ORF: 1563 bp
• Locus ID: 26427
• UniProt ID: Q9Z125
• Cytogenetics: 2 E1
• Gene Summary: Transcription factor involved in unfolded protein response (UPR). Binds the DNA consensus sequence 5'-GTGXGCXGC-3' (By similarity). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. Directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. Does not regulate COL1A1 in other tissues, such as skin (PubMed:19767743). Required to protect astrocytes from ER stress-induced cell death. In astrocytes, binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation (PubMed:15665855). Inhibits cell-cycle progression by binding to promoters and activating transcription of genes encoding cell-cycle inhibitors, such as p21/CDKN1A (By similarity). Required for TGFB1 to activate genes involved in the assembly of collagen extracellular matrix (By similarity).[UniProtKB/Swiss-Prot Function]