Cntfr (NM_016673) Mouse Untagged Clone

CAT#: MC205483

Cntfr (untagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cntfr
• Synonyms: Cntf; Cntfralpha
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046974
  CGGCAGTGGGATCCGGCCGCAGTGCTCGCTCCGCGCTCCTGCCTCGCTCCCCGCCTGCAGCGGCCGAAGG GCGCGGCGCGGAGCCCGGGTGGCCCGAGGGCGCGAACCCTGCCCCGTCACATCATCCCAGGAAGACTTTG GTCTGGAGAGGAGGATAATATTGATGTGTGTGGAGAACAGCTGGTGGTAACGAGATGGCTGCTTCTGTCC CATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGAAACACAGTCCACAGGA GGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGGGACAGCGAGCTGGGAC GCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGATAC TGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACTCCTGGCACCTGCGCCA CCAAGTCCTTCTACATGTGGGTTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACTTACCCC AAGGGCTTCTACTGCAGCTGGCACCTGCCCACCCCCACCTACATCCCCAATACCTTCAATGTGACTGTGC TGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCTGCCACATTCGGTACAT GCACCTGTTCTCAACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAACGCCTTGGGTCACAACACTACG GCCATCACCTTTGACGAATTCACCATTGTGAAGCCTGATCCTCCAGAAAACGTGGTGGCCCGGCCAGTGC CCAGCAACCCCCGTCGGCTGGAGGTGACATGGCAGACACCCTCAACTTGGCCCGACCCTGAATCCTTTCC TCTCAAGTTCTTCCTGCGCTACCGGCCTCTCATCCTGGACCAATGGCAGCATGTGGAGCTCTCGGATGGC ACAGCACACACCATCACAGATGCCTACGCAGGGAAGGAATACATCATCCAGGTGGCAGCCAAGGACAATG AGATTGGGACATGGAGTGACTGGAGCGTGGCTGCTCACGCCACACCCTGGACTGAGGAACCTCGGCATCT CACAACAGAAGCCCAGGCCCCCGAGACCACGACCAGCACCACCAGCTCATTGGCACCCCCACCCACCACG AAGATCTGTGACCCTGGGGAGCTTGGCAGCGGCGGAGGACCCTCCATACTCTTCTTGACCAGTGTCCCAG TCACTCTGGTCCTGGCTGCCGCTGCTGCCACAGCCAACAATCTCCTGATCTGAGCCCTGCATCCCACGAG GACATGCCAGAGCGCCTGCGGAGGAGCAGGAGGCCGGAGCTGAGCCTGCAGACCCCGGTTTCTATTTTGC ACACGGGCAGGAGGACCTTTTGCATTCTCTTCAGACACAATTTGTAGAGACCCCGGCAGGCCCGGGCCTG CCTTCCGTCCCCCCAGCCTCATCTCACCAAGCCGGCCCTCCTTTCCATCCAGAGAGGAGGACCATGCAGC TAACCCACCCACCAAAGACCCCTCTCATCTTTCCCCCCTCGGGCTGGACCCTCCAACGCCAGCGACTCCC AGGAGCCCTTGGGGGACCTGAGGGGAGCCCCCACCCCACATCCACAGTTTTCTCCTCCTGCCCCAGCCTC CTGTCTGTCCCAGGGTCTTTGTTGCCACCATCAGATTATAAGCTCCTGACACTGGGGGGGCCCAGCCATC CCCCTCCCCCCGGTGCCCACACTTTTCAGTCCTTCCACCTTTGCCCCTGTTTTGTACGATCCTCCATTGG CCCTTTCCTACCCCCCAGTATTTAATGTCCTGTCAGTCCCTTCTAGTCTGACTCAATGGTAACTTGCTGT ATTTGAATTTTTTATAGATGTATATACATGGGAGGGAGGGGTAGGGAGTTCTCATTAAACGTCACCATTT CATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016673
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046974, AAH46974
• RefSeq Size: 2013 bp
• RefSeq ORF: 1119 bp
• Locus ID: 12804
• UniProt ID: O88507
• Cytogenetics: 4 21.81 cM
• Gene Summary: This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.