Ehd3 (NM_020578) Mouse Untagged Clone

CAT#: MC205480

Ehd3 (untagged) - Mouse EH-domain containing 3 (Ehd3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ehd3
• Synonyms: Ehd2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046596
  GATGAACCTGGGACTGGACTGAATCCAGCAGCTGGGGCAGCCCGGGCCATGGTGCGTCTGAGCCCCGAGC TCTGGTAAGGCAGTAGTGGCGGCGTTGTTCGAGCTAGGCGGGTGCCACTCCCGGGAGCTTCCTCCCAGCT GTCACAATGTTCAGTTGGCTGGGTAACGATGATCGCCGCAAGAAGGACCCTGAGGTCTTCCAGACGGTGA GCGATGGACTCAAAAAACTCTACAAGACCAAGCTGCTGCCTCTGGAAGAGTATTACCGCTTCCACGAGTT CCACTCGCCCGCCCTGGAAGATGCTGATTTCGACAACAAGCCCATGGTCCTGTTGGTGGGCCAGTACTCT ACCGGCAAGACCACCTTCATCAGGTACCTGCTGGAACAGGATTTTCCAGGCATGAGGATTGGGCCTGAGC CGACCACTGATTCCTTCATAGCAGTGATGCAGGGAGATGTGGAGGGGATCATTCCCGGGAATGCCTTGGT GGTGGATCCAAAGAAACCCTTCAGAAAGCTCAACGCCTTTGGCAACGCCTTCCTGAACAGGTTTGTGTGT GCCCAGCTGCCCAACGCCGTGCTTGAAAGTATCAGTGTGATCGACACACCGGGGATCCTCTCTGGGGAGA AGCAGAGGATCAGCCGAGGGTATGATTTTGCTGCGGTCCTCGAATGGTTTGCTGAGCGGGTGGACCGAAT TATCCTACTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTCTCAGAAGTCATCAAGGCTCTCAAG AACCATGAGGACAAGATGCGCGTAGTGTTGAACAAAGCTGACCAGATCGAGACCCAGCAGCTGATGCGAG TATACGGAGCCCTCATGTGGTCCCTGGGGAAGATCGTGAACACCCCCGAGGTGATCCGGGTCTACATTGG CTCCTTCTGGTCCCACCCACTCCTCATTCCCGACAACCGGAAGCTCTTTGAAGCTGAAGAGCAAGACTTG TTCAGAGACATTCAGAGTCTACCCCGTAATGCTGCTCTTCGAAAGCTCAACGATCTCATCAAGAGAGCCC GGCTGGCCAAGGTCCACGCCTACATCATCAGCTCCTTGAAGAAGGAGATGCCCTCAGTGTTTGGGAAGGA CACCAAAAAGAAAGAACTGGTGAACAACCTGGCTGAGATCTATGGCCGGATTGAGCGAGAACACCAGATC TCCCCTGGAGACTTCCCCAACCTGAAGAGGATGCAGGACCAGCTGCAGGCCCAGGACTTCAGCAAATTCC AGCCACTGAAGAGCAAGCTGCTGGAAGTGGTTGATGATATGCTGGCTCATGACATTGCCCAGCTCATGGT GCTGGTGCGCCAGGAAGAGACCCAACGGCCTGTCCAGATGGTGAAGGGCGGAGCATTTGAGGGAACCTTA CAAGGCCCCTTCGGGCACGGCTATGGAGAGGGAGCTGGGGAGGGCATCGATGATGCCGAGTGGGTGGTGG CGCGGGACAAGCCTATGTATGATGAGATCTTCTACACCTTATCCCCAGTGGATGGCAAGATCACAGGTGC CAACGCCAAGAAGGAGATGGTGCGCTCCAAGTTGCCCAACAGCGTGCTGGGCAAGATCTGGAAGCTAGCC GACATTGACAAGGATGGCATGTTGGATGACGAGGAGTTTGCCCTGGCCAACCACCTTATCAAAGTCAAGC TAGAGGGGCATGAGCTGCCCAGTGAGCTACCTGCCCACCTCCTCCCTCCATCTAAGAGGAAAGTATCAGA ATGAGAGAGCCAGGTAACCTCAGACAGACAGTATCAAAAGAGAGGATAGACATGTAGACCACACACACAC ACACACACACACACACACACACACACACACACAACTTGACAGTCACACTATAAATGAGAAGGGTTCACCT TTGTCTGAGCACCTCTCCAAGTTCCCAGGGTTGGTAGAAGGGCAGCTTTCCCTCCTCTGTCTTAGGATAT AGGCCTGTGTCCAAACATTCCCTCCATCTTCCATTCCCCCACAGACATGAGGCAGTTAACACAGATGGCC CACCCACTCTACCCCCAGTGCCTCCACATCTAGGCTCCGAGCAGATGGAAAAGGCTTTTTCATGGAATAG AAAATTTGCTTTATTTTCTATGCTTTTATTTTTTTTCCCTCTGGGGCTTCCTAAGTAGAAATTGACTCAG GGCCTGGGAGCTGTGAGGGAAAGGAGAAGCTGAAAGAGGAGGACCAATCTGAGAAACCTCCATAGGGCAC TGCACCCCACACTTGAAAAGACACTGGCCTATGTTCTCTGTGTTTTTCTCAACCCAAGACTCTCTGTCTT CCTCAGTAAACATGGACCTTGAATTCTGCCTGCCACTTTGGGTCAAAGACTCACAAACAGGAAAAGAAAA AAGAAAAATTTGGTAGGAAAGCACAAGGAAGATACCCTGTGGTCTTCTTCAACAGGACATTGGATTGGTG GTTCATGGGTTTGTCCCCCACCCCCAGCGTGGTATCTCTGGATACTCAGTTTCTTTATACATACCAAGCC ATTCCTGTGTGGCAAGAGCAGGGTTAGGCACTTTCTATGTATTAGTCCCTGTGGCCTTCATGAATGCCCT AGGCAAGTTTGCTTCCCTCCTGTTACTGCATTTTTCAGGTGAAGAGCCAAAGACTCAGAGTAGTTTAGGG TACCTTCCCAAACTCCGGGAAGTCCCAAGAAGAGAAGATTCAAATCCAGAACTTGAGACACCCCTCTGTC CCAGTTCTGTGATGGATGAAAGATCCCAGTGTTGCTACGTGGTGACAAAGCACAGGACAGTCTGAACACA CAGCCCCTCACACAGCCTTCCAAAGCATCCAGGCAAGGGAGGGAGGGAGGTTCACCAGCCTTTGATGGGC CAACAATCTGACCATCTGTCACCTTGTAGAAGCAAACTGTGCCTTCTGGCCTGCGCCTCGTGTTCACAAC ATCACAGAAGACCAGCCAAGCCATCAGGAGAGTGGGCTGGACTGCTAGATGTTGTCTGTGCCTATTCCTG CTCAGCCTCCCGTTCATTAGCCTAAAGCATCCCAGCTCAAATTCAGCCCCAGGCTTTTACAAAGCAGGAC TTCATGCTAATTCACAGAAGGCCATCTTGAAAGGACTGGGACCTTGTTCTCTAGAGTTCCAAGGACTCTG GTGTCCTTGGCAAAATTTCCATCATTCTCAGTGCCCTCTATCTCCTCTGTGGTCTCCCCCTGGCTTGCCC TATGCCCACTGTTGCAGTAGCTCTCTGCTACACTCCTACTGTGATGGAAAACAAAGCAAGTATAACTTAT TTTGTATCTATGTTCAGACTATATCGACTGTTCTGTGTATCTTCAATGTGCTTATAACTGCAGTGTGTTT GTCATTAGGATTCATGTTAATACAACATATTTACCCTTTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020578
• Insert Size: 1608 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046596, AAH46596
• RefSeq Size: 3448 bp
• RefSeq ORF: 1608 bp
• Locus ID: 57440
• UniProt ID: Q9QXY6
• Cytogenetics: 17 45.2 cM
• Gene Summary: ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes (By similarity). Binding to phosphatidic acid induces its membrane tubulation activity (PubMed:26896729). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology (By similarity). Involved in the recycling of internalized D1 dopamine receptor (By similarity). Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (PubMed:20489164, PubMed:24759929, PubMed:25825486). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (PubMed:21408024). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with Ehd1 (PubMed:25686250).[UniProtKB/Swiss-Prot Function]