Smarce1 (NM_020618) Mouse Untagged Clone

CAT#: MC205458

Smarce1 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (Smarce1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smarce1
• Synonyms: 2810417B20Rik; 5830412H02Rik; 9030408N19Rik; Baf27
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC047141
  GCGAAGCCGGAGCTGCGGGCGCCTGCTCGGCCCGCGGTGTCTCAGATTCATTCTTAAGGAACTGAGAACT TAATCTTCCAAAATGTCAAAAAGACCATCTTATGCCCCACCTCCCACCCCAGCTCCTGCAACACAAATGC CCAGCACACCAGGGTTTGTGGGATACAATCCATACAGTCATCTCGCCTACAACAACTACAGGCTGGGAGG GAACCCGGGCACCAACAGCCGGGTCACGGCGTCCTCTGGCATTACGATTCCAAAGCCTCCAAAGCCACCA GATAAGCCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGACCAAGTAAAGGCTTCCAACCCTG ACCTAAAGTTGTGGGAGATTGGCAAGATTATTGGTGGCATGTGGCGAGATCTCACTGATGAAGAGAAGCA AGAATATTTAAACGAATACGAAGCAGAAAAGATAGAGTACAATGAGTCTATGAAGGCCTACCATAATTCC CCTGCGTACCTTGCCTATATTAATGCAAAAAGTCGTGCGGAAGCTGCATTAGAGGAAGAAAGTCGACAGA GACAGTCTCGCATGGAGAAAGGAGAACCTTACATGAGCATTCAGCCTGCTGAGGATCCAGACGACTATGA TGATGGCTTTTCAATGAAGCACACAGCCACTGCCCGTTTCCAGAGAAACCACCGTCTCATCAGTGAGATC CTCAGTGAGAGTGTGGTACCTGATGTGCGGTCGGTTGTCACAACAGCTAGAATGCAGGTCCTCAAGCGAC AGGTCCAGTCTTTAATGGTTCATCAGCGGAAACTAGAAGCCGAGCTCCTTCAGATAGAGGAACGACACCA GGAAAAGAAGAGGAAATTCCTGGAAAGCACGGACTCCTTTAACAATGAACTTAAAAGGTTATGTGGTCTG AAGGTAGAAGTAGACATGGAGAAGATTGCGGCTGAGATCGCACAGGCGGAGGAACAGGCCCGCAAGAGGC AAGAAGAGAGGGAGAAGGAGGCAGCAGAGCAAGCTGAGCGCAGTCAGAGCAGCATGGCCCCTGAGGAAGA GCAAGTGGCGAACAAAGCCGAGGAGAAGAAAGATGAGGAGAGCATCCCGATGGAGACAGAGGAGACACAC CTTGAAGACACAGCAGAGAGCCAGCAGAATGGTGAAGAAGGCACGTCTACTCCTGAGGACAAGGAGAGTG GGCAGGAGGGGGTTGACAGCATGGAGGTGGAAGGGACCAGTGACAGTAACACGGGCTCAGAGAGCAACAG CGCCACAGTGGAGGAGCCGCCCACAGACCCAGTGCCAGAAGACGAGAAGAAGGAGTAAATGTTGCCTTGT TTTATGTGACCTAAAACTTTTTTAAATGAAAAAAAAATGTGGTTTTTTTTTTTTTTAATGGTGTTATGTG GTCTGTGTATTAATTATTTACTTTTCCGTTGATACAACATGAAGGTCTTTGAACCCTCAGCATCATAGCC TAATGCCAGCCGCTCACCTTTCTTAGCTCTCAACGTCTGAAATCTCAGAGCTGAGATTAATCAAGAAGAC ACCCATCATTCTCTGAGAACTACCTTGGCTGCTGCAGAATCGACTCTTCCAAATACCTGCCTTCAGCTCA CGTGGTGCTCACCAAAGCCATAGCTTTAAACCCTTCCAGCCCATCCACAGCTTTCCCAGTCCCTGTCTTG TGTACTTACACAGAGTGCCCTCTTGAAATCATGAGGGGGTCTCTTCACTCACCCTTTCTATGTCCCATGT CAGACACCAGGAGTTCTCTTACAGGGTAGGGTGTAGCCAGAAACTGGTGAGACACAGATCACAGAGATGC CTCTGGGGGCACTGGGGGTGGGGGAGCAGGGGGAGTACAGTTGTTCTTTCTGTGGATTCCTTGTTGGTGA GAGCTGCGCCTGCTTATCTAGAGTGCTGTTCAGTGTAGTCGATCTGGGATGTGTTCTGGGAAATTCATCC TTTTTGTACAGGGGAAAGAAACACTTTTTTTTACCAGATTGGCTTTCCAAAGACACGATAGATGGCAGAG CTTAAGGAATGGAATGTTCTTATAATGGACTACAGACTTCAAAGTGATTGGCCCATTCCAAAAGGAAAAT GGGAATGCCGTTCATCCATGTGAGCATACTTCACAGTGATGAAAACCTCAAGACTCGAGATCCCATAGAT CAGAGCCGAACCTACTTTTTTGATAACCCCTGTAGTGGTCTTAGAGACTAGAAACAAGATAGTTTGTAGT GTGTGCTCCCTAAAATCTAGAATAGATTTTTACTGAATAGTGGTATATATGATGGTATATGTTTCTTAAA GGTCCAAACATAATAAAGAAATTAAGACAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020618
• Insert Size: 1236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC047141, AAH47141
• RefSeq Size: 2355 bp
• RefSeq ORF: 1236 bp
• Locus ID: 57376
• UniProt ID: O54941
• Cytogenetics: 11 D
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:12110891). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs)(PubMed:12145209).[UniProtKB/Swiss-Prot Function]