Rad54l (NM_009015) Mouse Untagged Clone

CAT#: MC205394

Rad54l (untagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad54l
• Synonyms: RAD54
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021643
  CCACGCGTCGGGAAGAATAAGGGGAAAGATACAGGAGTCGCTCTCTGCCTCTCTTCAGCTTCTAAGATTC TCCTCCTCCCAACCTGGATTTTTAACTGGTCCTTCCTGGGTTCCTGTTTAACTTCCCACAAGGCGTTCCT AGGTCTCTGCTTGCCTTCCTCACACTGAGGTTTGATGTCGGTCCAGCTCTGGTAAATTCTATCCCTCTCC TAAGAATTCAGGCGTCTCGCCTTCGTACTTAACACTGCACTTTTTTCGTAGGTGTTACTCTCAGGGAAGC AGCCCAGCTAGGGACGTTATTCCAACTCCACTTTGCTCATCTGCAACTTCAATCTGTCTTCTTTCCCATC CTAGATTTAGACCCAGCTCCGCCTGGACTTGGCTTTGTATATTTTGACCTTGAGGAACTTGGGGACCGAC ATTCCTGGTTTGGACCCAAGCCTCATTCCCTGATTGCATCCCCTCCCCTCATACCGACTCTTAAGACAAT ATTTTCAATGATATAGCAACTTCCTTTTATAGCATCCCCTGGTCCTGCATTCTCTGTTGCTGAGTGCTTT TAACTTAGCTGATGGGCACTTGACATCTGATGCTTCTGGGGCCAGGATGAGGAGGAGCTTAGCTCCCAGC CAGTTGGCCAGGAGGAAACCAGAAGACAGATCATCAGATGATGAAGACTGGCAGCCTGGGACAGTAACTC CTAAGAAACGCAAGTCCAGCAGTGAGACCCAGGTCCAGGAGTGTTTCCTGTCTCCTTTTCGGAAGCCCTT GACTCAGCTACTCAACCGGCCACCTTGTCTGGATAGCAGTCAACATGAAGCATTTATTCGAAGTATTTTG TCAAAGCCTTTCAAGGTCCCCATCCCAAATTATCAAGGTCCCCTGGGCTCTCGTGCATTGGGCCTGAAAA GGGTTGGAGTTCGTCGTGCCCTTCATGACCCTCTGGAAGAAGGTGCCTTGGTTCTCTATGAGCCTCCCCC ACTCAGCGCCCATGACCAACTGAAGCTTGACAAGGAAAAACTCCCTGTTCATGTGGTTGTTGATCCTATT CTCAGTAAGGTATTGCGGCCTCATCAGAGAGAGGGAGTGAAGTTCCTATGGGAGTGTGTCACCAGTCGTC GAATTCCTGGAAGCCATGGCTGCATCATGGCTGATGAGATGGGCCTGGGGAAGACACTGCAGTGCATCAC ATTGATGTGGACACTTTTACGCCAGAGCCCAGAATGCAAGCCAGAAATCGAGAAGGCAGTGGTGGTGTCA CCTTCTAGCCTGGTGAAGAACTGGTACAATGAGGTTGAGAAATGGCTTGGAGGGAGGATTCAACCTCTGG CCATCGATGGAGGCTCGAAGGACGAGATAGACCGAAAACTGGAAGGATTCATGAACCAGCGTGGAGCTAG AGTGCCTTCCCCCATCCTCATCATTTCCTATGAGACTTTCCGCCTGCATGTTGGAGTTCTTAAAAAAGGA AATGTTGGACTGGTCATATGTGACGAGGGCCACAGGCTAAAGAACTCTGAGAATCAGACTTACCAGGCTC TGGACAGCTTGAATACCAGCCGGCGGGTGCTAATCTCCGGGACCCCCATCCAAAATGATTTGCTGGAGTA TTTCAGCTTGGTGCACTTTGTAAATTCTGGCATTTTGGGAACTGCCCATGAATTCAAGAAGCATTTTGAG TTGCCAATTTTGAAGAGTCGAGATGCAGCTGCCAGTGAGGCAGACAGGCAGCGTGGGGAGGAGCGTCTGC GGGAGCTCATCGGTATTGTGAACAGGTGCCTGATACGGAGAACATCTGATATCCTCTCTAAGTATCTGCC CGTGAAGATTGAGCAGGTGGTTTGTTGTAGGCTGACACCCCTTCAAACTGAGCTATACAAGAGATTTCTG AGACAGGCTAAGCCTGAAGAAGAATTGCGTGAGGGCAAGATGAGTGTGTCTTCCCTGTCTTCTATCACCT CTCTAAAGAAGCTGTGTAATCATCCAGCTCTAATCTATGACAAGTGTGTGGCAGAGGAGGATGGCTTTGA GGGCACTTTGGGTATCTTCCCACCTGGTTATAACTCTAAAGCTGTAGAGCCACAGTTGTCAGGTAAGATG CTGGTCCTTGATTACATTCTGGCCGTGACTCGAAGCCGTAGCAGTGACAAAGTCGTGCTGGTGTCTAATT ATACTCAGACATTGGATCTCTTTGAAAAGCTGTGCCGGGTTCGAAGGTACTTGTATGTTCGCCTGGATGG CACGATGTCCATTAAGAAGCGAGCCAAGGTTGTGGAGCGCTTCAATAGCCCATCGAGCCCTGATTTTGTT TTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGTCTTAACCTCATTGGTGCTAACCGACTGGTCATGTTTG ATCCTGACTGGAATCCAGCCAATGATGAACAAGCTATGGCCCGAGTCTGGCGTGATGGTCAAAAGAAGAT CTGCTATATCTACCGACTGCTATCTGCAGGAACAATTGAGGAGAAGATCTTTCAGCGGCAGAGCCACAAG AAGGCTCTGAGCAGCTGTGTGGTGGACGAGGAGCAGGATGTGGAGCGCCACTTTTCTCTTGGTGAGCTTA AAGAGCTGTTTACTCTGGATGAAGCAAGCCTCAGTGACACACATGACAGATTGCATTGCCGCCGTTGTGT AAACAACCGTCAGGTCTGGCCACCCCCTGATGGTTCTGACTGCACTTCAGACCTGGCTCAGTGGAACCAC AGCACAGATAAACGAGGGCTCCAGGATGAGGTACTCCAGGCTGCCTGGGATGCTTCATCTACAGCCATCA CCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGCGGTCTTCACTGATAATCAGCTGGTCTGGATGT AGTTGTTTGGGAAAGGATACAGGGAAAGGGGGTTCTCTGCCTCATGAGGCTCTGCTCAGTTTTATTCTCT GGGAGAAAATCATCAAGAAGGGCTGCATGATGTTTGCCCAAAATTTATTTTATAAGAAAAACTTTTTTGG TTTAAAAAAAAAAAAAAAAAAAAAAG
• Restriction Sites: RsrII-NotI
• ACCN: NM_009015
• Insert Size: 2244 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021643, AAH21643
• RefSeq Size: 3036 bp
• RefSeq ORF: 2244 bp
• Locus ID: 19366
• UniProt ID: P70270
• Cytogenetics: 4 D1
• Gene Summary: Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). Deficient mice also show significantly shorter telomeres than wild-type controls, indicating that the protein activity plays an essential role in telomere length maintenance in mammals. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein.