Smurf1 (NM_029438) Mouse Untagged Clone

CAT#: MC205357

Smurf1 (untagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smurf1
• Synonyms: 4930431E10Rik; mKIAA1625
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029097
  CGGACGCGTGGGCTCGGGAGGCGGCGGCGGAAGCAGCGAGGGCGGCGGGCGTCCGGCTGCGAGCGCGGCG GCTCCGGGCTGGGCCAGCCAGGGGCGGCTCCTGGCGGCGGCGCGGCCGAGCGCTCGGTTCTACGGTTCCA GCCCGTGGTCTCCGGCCCAACGCCTGCGCCGCGCACTCGGCGTCCCGGCTCGGACCCCGGCGCCCAGCCC GTGGCCGTAACCTTGAGGCGGCGGCGGGGCCGGGCCGGGCCGGGCTGGGGGGCGGCGGCACTGGATCCGA GGCCGGCCGGCCGCTGGCGGGAGATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCG TCTGACAGTATTGTGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAG ATTGTTGTGGACGGCTCTGGGCAGTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGA GCCAGCACTATGACCTGTATGTTGGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGAT CCACAAGAAGCAGGGGGCTGGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAA GACACTGGCTACCAGCGTTTGGATCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAA TAGTGGTCAGTTTACAGACCCGAGACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCT GGAGAACGAAGGAACAGTGTATGAAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCT GCCCCGTATACAGATGGTACTGGTGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCC AAGATCAGAGACTCCTGGTACAGCGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAA CCGACCACATGGCCACCAGTCGCCAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAA GTTTACTTTTTGCACACGCAGACTGGAGTCAGCACATGGCATGACCCCAGGATCCCCAGAGACCTTAACA GTGTGAACTGCGATGAACTTGGGCCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAAT CTATTTTGTAGATCACAATAATAGGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCAC CAGTGCCAACTCAAGGAGCCCAGCCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGC TTCCTGCCCAGAGATATGAGAGGGACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCA GCAGCCCCAGGCTGGTCACTGTCGAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAG ATCATGAAGATGCGGCCAAAAGACCTAAAGAAGCGCCTCATGGTGAAGTTCCGAGGGGAGGAAGGTTTGG ACTATGGTGGAGTGGCTCGGGAGTGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACT CTTCCAGTATTCCACGGACAATATTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCAT CTGTCTTACTTCCACTTTGTGGGTCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGG GTTTCACAGTTCCGTTCTACAAGCAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGA CCCAGAACTGCACAAGAGCTTGGTGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTC TGCGTGGAGCACAACGCTTTCGGGCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTG TCACTGAGGAGAACAAGAAGGAATACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGC CCAGTTCTTAGCACTTCAGAAGGGGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAG AAGGAACTAGAGCTGATAATAGGTGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGC TGAAACACTGTGTGGCAGACAGCAACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGA GAGGAGAGCCAGACTCCTGCAGTTTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTG CAAGGCGCGGCAGGGCCCCGGCTGTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGG CCCATACCTGCTTTAATCGGATCGACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCT GACAGCGGTGGAGGAAACCTGTGGCTTTGCAGTGGAGTGAAGAGCAGCCAGTGGCCACAGAGTCTTGCTC ACAACCACCAGACCCAAAGCGTATGGCGTCTGCACGCCTCCCGCGTGGCGGGCTGAGGCCTGAGGTTCCA GAAACCAAGGGAAAAGGCTCGTCTCCCTCCTCCTTTGGAGAGGGCAGGCCAGGGGACTTTCCTAGGTGGC TCCCACCCATTTATTCTCCTTTATTATAGTTTGCCCACCCCTCCATCACCCATCCAATAAAACGCAGCCA GGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029438
• Insert Size: 2187 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029097, AAH29097
• RefSeq Size: 2765 bp
• RefSeq ORF: 2187 bp
• Locus ID: 75788
• UniProt ID: Q9CUN6
• Cytogenetics: 5 G2
• Gene Summary: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single C57BL/6J strain transcript was available for the full length of the gene. The extent of the sequence in this record is supported by orthologous human data.