Tnrc6b (NM_177124) Mouse Untagged Clone

CAT#: MC205301

Tnrc6b (untagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnrc6b
• Synonyms: 2700090M07Rik; A730065C02Rik; AI848765; D230019K20Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068158
  AAAGAATTCATTTTTTGGACCTTTTTTCATTTCCATTTCTACCTTGTATGCCTCAATTTGCTGGATTTAA GCACTGCTGCACTTTATGAGAGAGAAGGAGCAAGAAAGGGAAGAACAGTTAATGGAAGACAAGAAAAGGA AGAAAGAGGATAAAAAGAAAAAAGAAGCCACCCAGAAGGTCACGGAACAAAAAACCAAAGTGCCCGAAGT GACGAAACCAAGTTTAAGCCAACCAACGGCCGCCAGCCCAATTGGCAGCTCTCCATCGCCACCAGTCAAT GGTGGCAACAATGCCAAAAGGGTGGCAGTGCCGAACGGACAACCGCCAAGCGCCGCCCGCTACATGCCTC GGGAGGTGCCACCGCGATTCCGTTGCCAGCAGGACCATAAAGTGTTACTAAAACGTGGGCAGCCCCCTCC ACCGTCCTGCATGCTCCTCGGGGGTGGGGCGGGGCCTCCTCCCTGCACAGCACCTGGAGCAAACCCCAAC AACAACGCACAAGTGACAGGAGCGCTGCTGCAGAGTGAGAGTGGGACTGCACCAGAGTCAACCCTTGGAG GTGCTGCTGCTTCAAATTATGCAAATTCCACTTGGGGCCCGGGAGCCTCCTCCAACAGCGGCGCCTCCCC CAACCCAATTCACATCTGGGACAAGGTGATTGTAGACGGGTCTGACATGGAAGAGTGGCCTTGTATTGCC AGCAAAGACACTGAATCTTCTTCCGAAAACACCACCGATAACAACAGTGCCTCGAACCCTGGCTCTGAGA AGAGCAGTCTGCCAGGAAGCACCACTAGTAACAAAGGCAAAGGGAGCCAATGCCAGGCTGCAAGTTCTGG GAACGAATGTAATCTTGGGGTCTGGAAATCTGACCCCAAGGCTAAGTCTGTTCAACCTCCCAACTCTACT TCAGATAGCAACAATGGACTAGGAAACTGGAGGAGCACGAGTGGTCAGGATAGAATTGGACCTGGCTCTG GCTTCAGCAACTTTAACCCAAATAGCAACCCATCTGCCTGGCCAGCATTGGTCCAAGAAGGGACTTCTAG AAAAGGGGCATTGGAAACAGAAAGTAGTAGTTCCAGTGCACAAGTTAGCACAGTAGGTCAGGCATCCAGG GAACAGCAGTCAAAGATGGAAAATGCGGGTGTTAATTTTGTTGTCTCTGGCAGAGAACAGGCTCAAATTC ATAACACTGATGGACCAAAAAATGGAAACACTAACTCCTTGAACTTAAGTTCACCAAACCCCATGGAAAA TAAGGGAATGCCCTTTGGAATGGGCTTGGGGAACACCTCCAGGAGCACTGATGCCCCTTCACAAAGCACT GGAGATCGAAAGACTGGGAGTGTTGGGTCTTGGGGTGCAGCTAGGGGGCCTTCTGGAACTGACACAGTCT CTGGACAAAGCAATTCTGGAAATAATGGGAACAATGGAAAAGACAGAGAGGACTCCTGGAAAGGAGCTTC TGTTCCAAAACCAACTGGGTCAAAAAGTGACTCTTGGGACAACAATAACAGGTCTACGGGTGGGTCCTGG AACTTTGGCCCTCAGGACAATAATGACAACAAATGGGGTGAAGGGAACAAAATGACATCTGGGGTCTCTC AGGGAGAATGGAAACAGCCGACTGGGTCTGATGAGTTGAAAATTGGAGAATGGAGTGGTCCAAACCAACC AAATTCTAGCACTGGAGCATGGGACAATCAAAAGGGCCACCCCCTCCCTGAAAACCAAGGCAATGCCCAG GCTCCCTGTTGGGGAAGATCTTCCAGCTCCGCAGGAAGTGAAGTTGGAGGTCAAAGCACTGGAAGCAACC ACAAAGCAGGAAGTAGTGACAGTCATAATTCTGGTCGTCGGTCATATAGGCCCACGCATCCTGATTGCCA GGCTGTCTTGCAGACTCTTTTGAGCCGGACTGATTTGGACCCTAGGGTGCTCTCAAACACTGGCTGGGGC CAAACTCAAATTAAGCAGGACACAGTGTGGGATATTGAAGAGGTGCCAAGGCCTGAGGGGAAATCTGACA AAGGAACTGAGGGGTGGGAGAGCGCTGCCACACAGACCAAGAACTCAGGGGGCTGGGGAGATGCACCCAG CCAAAGCAATCAAATGAAGTCTGGATGGGGGGAGCTCTCAGCCTCGACAGAGTGGAAAGACCCCAAGAGC ACAGGAGGCTGGAATGACTATAAGAACAACAACTCTTCTAACTGGGGAGGAGGCCGAGCAGATGAAAAGA CACCTTCCTCTTGGAATGAGAGTTCTTGTAAGGATCAGGGGTGGGGAGGTGGGCGCCAACCCAACCAAGG ATGGACTTCTGGGAAGAATGGTTGGGGCGAGGAGGTGGATCAAGTGAAAAATAATAATTGGGAAAGTTCT GCCAATAAGCCTGTGTCTGGGTGGGGTGAAGGAGGACAGAATGAAATTGGAACTTGGGGTAATGGTGGGA ATACAAACCTAGCATCAAAAGGTGGGTGGGAAGATTGCAAAAGATCCCCTGCATGGAATGAGACAGGCAG ACAGCCCAATTCCTGGAATAAACAACACCAACAGCAGCAGCAGCCACCGCCACCACAGCCAGAGGCTTCT GGTTCATGGGGAGGCCCACCCCCGCCACCTCAAGGCAATGTTCGACCTTCTAATTCCAACTGGAGCAGTG GACCCCAGCCTACAACCCCTAAGGATGATGAACCCAGTGGTTGGGAGGAGCCATCCCCACAGTCAATTAG TCGGAAAATGGACATTGATGATGGCACTTCAGCTTGGGGAGACCCTAACAGTTATAACTACAAGAATGTG AACCTTTGGGATAAGAATTCTCAAGGGGGCCCAGCACCTCGAGAACCAAACCTGCCTACCCCAATGACCG GGAAATCAGCATCAGTCTGGAGCAAAAGCACACCACCTGCTCCAGATAATGGGACTTCAGCCTGGGGTGA GCCAAATGAAAGCAGTCCTGGGTGGGGTGAAATGGATGATGCAGGAGCATCAACCACAGGCTGGGGAAAC ACACCTGCCAACGCTCCCAATGCCATGAAACCCAATTCCAAATCTATGCAAGACGGCTGGGGGGAGAGTG ACGGACCAGTCACAGGAGCTCGCCATCCTAGCTGGGAAGAGGAGGATGACGGAGGAGTCTGGAACACCGC CGGCTCTCAGGGAAGCACTTCCTCCCACAACTCAGCAAGCTGGGGACAAGGAGGAAAGAAACAAATGAAG TGCTCACTAAAAGGAGGAAACAATGATTCATGGATGAATCCTCTTGCCAAACAGTTTTCAAATATGGGAT TGCTGAGTCAAACTGAGGATAACCCAAGCAGCAAAATGGATTTGTCTGTAGATAAGAAATTTGATGTGGA CAAGAGAACAATGAATCTCGGGGATTTTAATGATATCATGAGGAAGGATCGACCTGGGTTCCGTCCACCT AATTCCAAAGACTTGGGAACCACAGACAGTGGGCCTTATTTTGAGAAGGGCGGCAGTCATGGTTTGTTTG GAAACAGCACAGCACAATCGAGAGGCCTGCACACTCCAGTGCAGCCACTAAGTTCTTCTCCTGGTCTCCG GGCGCAAGTGCCTCCCCAGTTTATCTCCCCCCAGGTTTCTGCCTCAATGCTCAAGCAGTTCCCCAACAGT GGCCTGAATCCAGGTCTCTTTAATGTGGGGCCCCAGCTATCTCCTCAACAGATTGCCATGCTGAGCCAGC TTCCACAGATACCCCAGTTTCAGTTGGCATGTCAGCTTCTCCTGCAGCAGCAGCAGCAACAGCAGCAGTT GCTACAGAACCAGAGAAAGATTTCTCAAGCAGTGCGCCAGCAGCAAGAACAGCAGCTGGCAAGAATGGTG AGTGCTCTCCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGAGGCAGCCTAGCATGAAGCATTCACCTT CTCATCCTGTTGGGCCTAAGCCACATTTGGACAACATGGTACCCAATGCGCTGAATGTGGGGCTCCCAGA CCTTCCAACCAAAGGGCCAATTCCTGGATATGGTTCTGGCTTCAGCTCTGGTGGCATGGACTATGGTATG GTTGGTGGCAAGGAGGCTGGGACCGAGTCTCGCTTCAAACAGTGGACCTCAATGATGGAAGGGCTGCCAT CTGTGGCCACACAAGAAGCCACCATGCACAAAAACGGCGCTATAGTGGCCCCTGGTAAGACCCGAGGAGG TTCACCATACAACCAGTTTGATATAATCCCAGGTGACACACTGGGTGGCCATACGGGTCCTGCTGGTGAT AGCTGGTTACCTGCCAAATCTCCACCGACTAATAAAATCGGAAGTAAATCCAGCAATGCCAGTTGGCCTC CAGAATTCCAACCAGGAGTGCCATGGAAAGGTATCCAAAATATTGACCCTGAATCTGACCCCTATGTCAC CCCAGGAAGTGTGCTAGGGGGTACAACCACATCTCCCATTGTAGATACTGACCACCAACTGCTGCGGGAT AACACCACAGGGTCTAATTCCTCCCTCAACACCTCGCTGCCTTCACCTGGTGCCTGGCCCTACAGTGCCT CTGACAACTCCTTTACCAACGTTCATAGCACTTCAGGTACTCGGTGGCGTTTCCCCTCTTGCTTAGCAGT TGACTGTGAGGCTCCTCTTTATTACCAGCATTTACTTTCTAAGGGGCACCACTTAAGGATGGGAAACACC TAAGTCAGCACAGATGGACACACAACCTTTCTTTGCATTCAGCATCTACTCAGGGAGTTCCTGGACTCCC AGAGATAGGATAGGTAACACAGGACTATATTGGACCAAGGCCAATGGGAATAGAATCTCTCATAACTGCA GTTCTTCAGAGGGCAGAGCCCATGACCTGATGACCAAATGTTTCTGTTAACTAGCCAGCCAAGAAACCAA GGGTTATACAGAGAGCCAGTTCAACCCATATTAACATTGAGGCAGCCCATGGGAACTTACGGACCTAAAT CTGACCTAGAGACATAATGAAAGTAAATGGCAGAGTAAAAAAGAGGACCTTGGTTAATTTGCCAATAGTA CTTGTTGAGTTCCTATGGTTGGTTGGCTGGTTGGTTTTTGCATTTATTTGCTTATTGAGGCTTAAGAAGG AATAGAAAAGTTAATAGGAGCTAGAGGGATGGTTCAGTGGGTAAAATGCTATGCAAACATGGAGACTTGA GTTCAGTCCCCATGCCCACATTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_177124
• Insert Size: 3204 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068158
• RefSeq Size: 5221 bp
• RefSeq ORF: 3204 bp
• Locus ID: 213988
• Cytogenetics: 15 E1
• Gene Summary: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the 5' UTR and 5' coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.