Eif2a (NM_001005509) Mouse Untagged Clone

CAT#: MC205270

Eif2a (untagged) - Mouse eukaryotic translation initiation factor 2A (Eif2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2a
• Synonyms: D030048D22; D3Ertd194e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC082557
  TGAACTCTCACCCCAGCGCGTGGGTTTCTCTTTCCGGGACAAGATGGCGCCGTCCACACCGCTGTTGACA GTCAGAGGATCAGAGGGACTGTACATGGTGAATGGACCACCACACTTCACAGAAAGCACGGTGCTTCCCA GAGAATCTGGAAGAAATTGCAAAGTGTACACTTTTAGTAAGGATGGGACATTGTTTGCCTGGAGCAATGG AGAAAAAGTAAATATAATCAATGTCGCTAACAAGGGACTACTGCACTCCTTCGACCTCCCAAAAGCAGTT TGCCTTGAATTCTCACCAAATAACACTGTCCTGGCAACGTGGCAGCCTTACACTACTTCTAAAGATGGCA CTGCTGGGACGCCTAACCTACAACTTTATGACATGAAAACTGGAGCATGTTTGAAATCGTTCATCCAGAA AAAAATGCAAAATTGGTGTCCTTCCTGGTCAGATGATGAAATTATTTGTGCCCGGAATGTTAACAACGAA GTTCACTTCTTTGAGAACAACAATTTTAACACAATTGCAAATAAATTACATTTGCAAAAAGTTAATGACT TTAATTTATCACCTGGAACCCAACCTTATAAGGTGGCAGTTTATGTCCCAGGAAGTAAAGGTGCCCCCTC ATTTGTTCGACTGTATCAGTATCCCAACTTTGCCGGACCTCAGGCAGCACTGGCCAATAAAAGCTTCTTT AAAGCTGATAAGGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGCTGGTAATAGCCAGTACAGAGG TTGACAAGACAGGAGCCTCCTACTATGGAGAACAAACACTGCACTACATTGCAACAAATGGAGAAAGTGC TGTGGTACAATTACCAAAAAATGGCCCAATTTATGATGTAGTTTGGAATTCCAGTTCTACTGAGTTTTGC GCTGTTTATGGTTTTATGCCTGCGAAAGCAACAGTTTTCAACCTGAAGTGTGATCCTGTGTTTGACTTTG GGACTGGTCCTCGCAACGCAGCCTTCTATAGCCCTCATGGACATATATTAGTGCTGGCTGGATTTGGAAA TCTTCGAGGACAAATGGAAGTATGGGATGTTAAAAACTACAAGCTTATTTCTAAGCCAGTGGCTTCTGAT TCTACATATTTTGCCTGGTGCCCAGATGGTGAGCATATCTTAACAGCCACATGTGCTCCTAGGTTACGTG TTAATAATGGGTATAAGATCTGGCATTATACTGGCTCTCTTTTGCATAAGTATGATGTGCCATCAAATGG AGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATATTTCCAGCAAAAACGATAAAGTACCAA GCAGTTCCAAGTGAAGTGCCTAGTGAGGAGCCTAAAGTTGCAACAGCTTATAGACCTCCAGCCTTAAGAA ATAAGCCAGTCACCAATTCCAAACTGCATGAAGAGGAACCTCCCCAGAATATGAAGCCACATCCAGGAAG TGACAAGCCATTATCAAAAACAGCCCTTAAAAATCAAAGGAAGCATGAAGCTAAAAAAGCTGCAAAACAG GAAGCAAGAAGTGATGCGGCTCCTACTCCTGTCCCACAGAGTGCACCACGGAACACGGTCACCCAGTCTG CTTCTGGTGATCCTGAAGTAGACAAAAAAATTAAGAATCTAAAGAAGAAACTGAAAGCCATAGAGCAGCT AAAAGAGCAGGCAGCCGCCGGGAAACAGCTAGAAAAAAATCAGTTGGAGAAAATTCAGAAAGAGACAGCC CTTCTCCAAGAGCTTGAAGATTTGGAATTGGGAGTTTGAAGACTCACAGAAAGTGAATTTGTTGGCAGGA AACACTATAAGCAAGTCTCTTAAGCCCATGGTGTTCATGGAATTGCCATATGCCCACTGTTAACATCAGT CTATATTTTTAACCATTTATCCAAGGTTGAATATGTATAATTATCTAATGATGTCTATTCAATTGACTTT ACATCTGCATTCTGTATCATGTCAGTATGTAATAAAGAGAGATGATTTTTTCCAGATAAGCTTAATAAAT TGACAGACATTTGGTTTTTTTTTTAAATGGGTAATAGTATTTCCTATGTAAACAAATAAAGACATTTTAA ATTTGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001005509
• Insert Size: 1746 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082557, AAH82557
• RefSeq Size: 2128 bp
• RefSeq ORF: 1746 bp
• Locus ID: 229317
• UniProt ID: Q8BJW6
• Cytogenetics: 3 28.58 cM
• Gene Summary: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.[UniProtKB/Swiss-Prot Function]