Bace1 (NM_011792) Mouse Untagged Clone

CAT#: MC205212

Bace1 (untagged) - Mouse beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bace1
• Synonyms: ASP2; Bace; C76936
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048189
  AGCCTGCCTAGGTGCTGGGAGCTGGGAGCTGGATTATGGTGGCCTGAGCAGCCGACGCAGCCGTAGGAGC CCGGAGTCCCTGTCGGTCCCCAAGCTGCAAAGCCCGCCTGGAAGACCCCGAAAGCTACGGGCTCGGATAG CCATGCCCGCCCCTCCCAGCCCCACAAGGGGCCCGATCCCCCCGCTGGCGGCCGGCGTCCAGATGTAGCT GGGTCCCCTGGATCGCCATCGTCGTCTCCTCTCGTGCGCTACGGATTTCTCCTGCCCACTCTCCGCCGCC TGGACCGGGAACTGAGCGAGGGGCCTGCAGACTCTGCAGTCCTGATGCCGCCGAGGCCGCTCTCCTGAGA GAAGCCACCACCACCCAGACTTAGGGGCAGGCAAGAGGGACAGTCACCAACCGGACCACAAGGCCCGGGC TCACTATGGCCCCAGCGCTGCACTGGCTCCTGCTATGGGTGGGCTCGGGAATGCTGCCTGCCCAGGGAAC CCATCTCGGCATCCGGCTGCCCCTTCGCAGCGGCCTGGCAGGGCCACCCCTGGGCCTGAGGCTGCCCCGG GAGACCGACGAGGAATCGGAGGAGCCTGGCCGGAGAGGCAGCTTTGTGGAGATGGTGGACAACCTGAGGG GAAAGTCCGGCCAGGGCTACTATGTGGAGATGACCGTAGGCAGCCCCCCACAGACGCTCAACATCCTGGT GGACACGGGCAGTAGTAACTTTGCAGTGGGGGCTGCCCCACACCCTTTCCTGCATCGCTACTACCAGAGG CAGCTGTCCAGCACATATCGAGACCTCCGAAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAGG GGGAACTGGGCACCGACCTGGTGAGCATCCCTCATGGCCCCAACGTCACTGTGCGTGCCAACATTGCTGC CATCACTGAATCGGACAAGTTCTTCATCAATGGTTCCAACTGGGAGGGCATCCTAGGGCTGGCCTATGCT GAGATTGCCAGGCCCGACGACTCTTTGGAGCCCTTCTTTGACTCCCTGGTGAAGCAGACCCACATTCCCA ACATCTTTTCCCTGCAGCTCTGTGGCGCTGGCTTCCCCCTCAACCAGACCGAGGCACTGGCCTCGGTGGG AGGGAGCATGATCATTGGTGGTATCGACCACTCGCTATACACGGGCAGTCTCTGGTACACACCCATCCGG CGGGAGTGGTATTATGAAGTGATCATTGTACGTGTGGAAATCAATGGTCAAGATCTCAAGATGGACTGCA AGGAGTACAACTACGACAAGAGCATTGTGGACAGTGGGACCACCAACCTTCGCTTGCCCAAGAAAGTATT TGAAGCTGCCGTCAAGTCCATCAAGGCAGCCTCCTCGACGGAGAAGTTCCCGGATGGCTTTTGGCTAGGG GAGCAGCTGGTGTGCTGGCAAGCAGGCACGACCCCTTGGAACATTTTCCCAGTCATTTCACTTTACCTCA TGGGTGAAGTCACCAATCAGTCCTTCCGCATCACCATCCTTCCTCAGCAATACCTACGGCCGGTGGAGGA CGTGGCCACGTCCCAAGACGACTGTTACAAGTTCGCTGTCTCACAGTCATCCACGGGCACTGTTATGGGA GCCGTCATCATGGAAGGTTTCTATGTCGTCTTCGATCGAGCCCGAAAGCGAATTGGCTTTGCTGTCAGCG CTTGCCATGTGCACGATGAGTTCAGGACGGCGGCAGTGGAAGGTCCGTTTGTTACGGCAGACATGGAAGA CTGTGGCTACAACATTCCCCAGACAGATGAGTCAACACTTATGACCATAGCCTATGTCATGGCGGCCATC TGCGCCCTCTTCATGTTGCCACTCTGCCTCATGGTATGTCAGTGGCGCTGCCTGCGTTGCCTGCGCCACC AGCACGATGACTTTGCTGATGACATCTCCCTGCTCAAGTAAGGAGGCCCGTGGGCAGATGATGGAGACGC CCCTGGACCACATCTGGGTGGTTCCCTTTGGTCACATGAGTTGGAGCTATGGATGGTACCTGTGGCCAGA GCACCTCAGGACCCTCACCAACCTGCCAATGCTTCTGGCGTGACAGAACAGAGAAATCAGGCAAGCTGGA TTACAGGGCTTGCACCTGTAGGACACAGGAGAGGGAAGGAAGCAGCGTTCTGGTGGCAGGAATATCCTTA GACACCACAAACTTGAGTTGGAAATTTTGCTGCTTGAAGCTTCAGCCCTGACCCTCTGCCCAGCATCCTT TAGAGTCTCCAACCTAAAGTATTCTTTATGTCCTTCCAGAAGTACTGGCGTCATACTCAGGCTACCCGGC ATGTGTCCCTGTGGTACCCTGGCAGAGAAAGGGCCAATCTCATTCCCTGCTGGCCAAAGTCAGCAGAAGA AAGTGAAGTTTGCCAGTTGCTTTAGTGATAGGGACTGCAGACTCAAGCCTACACTGGTACAAAGACTGCG TCTTGAGATAAACAAGAACCTATGCGATGCGAATGTTTATACTCCTGGGGGCAGTCAAGATGAGGAGACA GGATAGGATAGAGACAGGAAGGAGATGGTAGCAAAACTGGGAAAGGCAGAACTCTGATCACTTTCTAGTT CCAAGTTTAGACTCATCTCCAAGACAGAAGCCCATCTGGACTAAGAGGTATCATTCCCCAATGTGCCTGT GGTTGTAGTCTGAACTGAAATGAAATGGGGGAAAAAGGGCTTATTAGCCAAAGAGCTCTTTTTAACACTC TTAGAGGAACAGTGCTCATGAGAAAAGTCCCACTGGACAGATGAATTCCTATCTTGTTAATTCTGTCTCT CTCTGCTTCTTCAACATGCTAAGTGGCACCAAAATGACCCAACCCCAAGGTCTTAGGTGCCCTATGGGAC AACAGTTAGAATATTGTAGGGCTAGGGATGGTCTTCCCAGCATAGGTTCACTCCAACCAAGGTGCTAAAA GGAACAGACAGGAGAGTCCTCCTCTCTGATCCACAAAGGCAGAGCCCTCAAGATTCATCCAGCAGGGTTA GGGCTGATGCATTTGCCTCTGCCTGGATTTTGTTTTTATTTTCTTTCTTTTTGCCCAGTGGTACAAAACG ATAAGCTCTTTATGGAATACTGAGTGGGTTCATTCCTCTCTTGCCCTCTCCAATGGCCCCTCTATTTATC TGGCTAAGGAAACACCACGCATTGGCTAGTATTAAACAGCAACTGTAAGATAGAGGGCTTTCTGTTCTAT GTCATTGCCTTCAGTATCAAGGCTGCCTGGAGAAAGGATGGCAGCCTCAGGGCTTCCTTACTTTCTTCTC CTTTCCTGACAGAGCAGCCTTTCTGTCCTGCTCTCTGCTGCCCCTCCCAATATAATCCATGGGTACCCAG GCTGGTTCTTGGGCTAGGTTGTGGGGGCCACACTCACCTCTTCCCTGCCAGTTCTAACACGACAGACATG AAGCCAGTGTTAGTGGGAAGAGCTGGGTTTTCCCAGGATGACCACTGCATCCTCTCCTGGTACGCTCTAC ACTGCTTTCAGGCTGGGGACCTGCCAAGTGTGGGACAGTTGATGAGGAAGAGACATTAGCAGGGCCTCTG GAGTTGCTGGCCCAGCCAGCTGCCCACAAGCCATAAACCAATAAAATAAGAATCCTGCGTCACAGTTTCC AGCTGGGTCCTCTTCCTTGCCCTCGCACTGGTGCTGCTCTGGCTGAGTAGGAATACACCCACAGACTGCC AGGAAGATGGAGACTGTCCGCTTCCGGCTCAGAACTACAGTGTAATTAAGCTTCCAGGATCACTACCATG AAAACGCCGCATTCTGCTTTATCATTTCTACCCATGTTGGGAAAAACTGGCTTTTTCCCCATTTCTTTAC AGGGCAAAAAAAAAAAAAAAAAAAGGGAGAGAGAGAGAGAACTCAACCTAGTTGTTATTTACCCTAGTAA CTGGTGTTCTATTTTTTTTTAAAGGGGGAAAATTTGCATTTATTTTTCTTTTGATGGTTAACTCCTTTGT ATCATAAAATTATGAACTCTGATATGTAAAACAGAAAAAAATCTTGACAACAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_011792
• Insert Size: 1506 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048189, AAH48189
• RefSeq Size: 4059 bp
• RefSeq ORF: 1506 bp
• Locus ID: 23821
• UniProt ID: P56818
• Cytogenetics: 9 A5.2
• Gene Summary: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).