Sgo2a (NM_199007) Mouse Untagged Clone

CAT#: MC205210

Sgol2 (untagged) - Mouse shugoshin-like 2 (S. pombe) (Sgol2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgo2a
• Synonyms: 1110007N04Rik; 4932411A20Rik; 5730576N04Rik; AU018846; AV008062; D1Ertd8e; Sgol2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052742
  CAAGTGTGGGCACCCACAGCTGAAGGGGTCGCTGGTAGGCGTGGACGAGGCCAGCGCGGCCAGCAAGGCC TGCGAAGCCGGAACCCTTCTCGGTAGCCACTCCTGGCGCCTCAGTCCTCGCCGCCGCGCGTCGGCTTGCA GGGCCGGGACGCGGAGCCGGGACGCGGAGCCGCCTTCGGATCGGGGCGGTAACCTCTGTTCTGACTTTGA GCTCCCAGCTGGGCGAGGGGTCGCCACTAGTTTTCAGAAATGGAGTACCCAGGGATAAAAGTTGACACTG TTACCTCTGGAATTCAGAGACGAGTGAAGGGCAGAATTGCAAAGACAAATTTGAATGTTTCTCTTGCTTC AAAGATCAAAGCAAAAATATTAAACAATTCTTCTATTTTCAAGATCTCTCTAAAGCACAACAACAGAGCA TTAGCGCGGGCCCTTAGTAAAGAGAAAGAGAATTCTCGAAGAATTACTACCGAAAAGATGCAATTACAGA AAGAAGTAGAGAAACTGAATTTTGAGAATACCTTTCTTCGCTTAAAGTTAAATACCTTGAATAAGAAGCT TGTAGAAATAGAATCGCATGTGAGCAATGATTTGTTAACTGCAATTGAAATAAGCAGTCTTTCTGAGTTC CACCAAGGTTCTTTTCTCCTGTCAGCTACCAAGAAACAAAGGAACAGTAAGCAGTGCAAGCCTGCGCATC TTCCATATGCAAGAGTTCTGTTAACTTCAGAAAATGATGATGATGATGGTGCTGATGATAAATGGCAGAC AAAGTGTAACAACAGAACTATATCAAAGACCTCACCTGATAGTACCTCTTCAGTATCAAGACAACCTTCA TCCTTACATCAGTGCAATTTGAAAGCATTCCCTCCTAAAGAAGATAATCAGAAGACATGTGGGTCAGGTC ATTTAGAACATACTTCAAGTGTTGATATACTTCCTAATGAGAGCCACTCAGATCAAAGTCCTAAGAGTTC TCTGAGTGAGATGAAAACTGCTCCATCTCCCAGCCTCAGAAGGGAAAAATTATCACATGGTAATGTGACT ATGAGGAAGAAGTGTGTGTCTTCAACTCCAGACATTCTGTATGTGACAGATTTAGATCACCAACCAACTT CAAGTCCAGGATCAAATTGGAATAATGAGATACATGGTCATACTAATGAAACCAGCAATAACACGCAAAG AAATGCCGAGTGTTTTCTTGACTTACCTTCTGAGTCTTCCAGTGAGCCTGACGCAAAGCGCATGGAGCTA GTGCAGAAGAACACCGATAGCTTTCACTTCCAGAAAACTGTATATGATGCCGCTGATATGGAGTTAACTG CTACTGACATAGGCAAGATTGTAGCAGTTTCAAAAAGCAAGAAAAATCAAAATAAGAAAAAGGCAGACTG TAGAAAGGAGACTTTCAGAAAAGTGAAAGGTGCAAGCTCTGATAAAAAGAGAGAAAGCTCAAAGAGAGAA TGTAAAGATGGTTCAGAAGTAGGTGCTGAGGAAGAGGCTGATGCAGCCAGAGCAGAAAGAGGCGCTGGTG TCCTGGATGGCAGAGGGGATTCAGAAGAGCCAAACTGCATTTCCAGTACTGAGCAGCCATCTCAGGTAAA CACGCAAAAGAAAAGAACCCTCCAGAACAGCTCAGATCAGGAGAACATTCAAAATACGAAGAGGAGGCAA ACATATACGACAGATGAGCAAGAGGAAACAAACCCTTTCTCCAGACATTCAGTCAAATTTCTTCAAGATG GTAAATTTGATCTGTGTCAGAAAACCCTACATCATAATTTAAGTAAGCCTTCTCGACAGACATTTGTGAT TCGTAAGTCAGAAAAAGATAACTTATTTCCAAATCAAGAAGATAAAGACACCATTTCTGAAAACCTAGAA GTTACAAATGAATTTCATATAGATGATCTTTCCATCGAAGCTAATGAAAATGTATGTGACCATGAGACTC AGACAATGTTGGACTTGAAAAAGTCTGTCAGTGCTCAACAAAATCAAACAAAAATAAATAAGACTAAGCA GAAAATAAATCGAAGGACAAAAATAATTTCTGTCATGAGCCAAGTATATGAGGACAATGATAAAGATATT CACGTCCTAGAAAAAGACAACTTTCCCTTTCATACCCAAGCAAATAAAGAAACCACCAGTGGAAACCTAG AAAGTTCAAAAGAATTTGAATCACCTCTTCTTTTCACAAGAGACAACGGAAGCTTACGTGACTGTAAGAC CCAGAATGTTCTGGATCTGCACAAGCAAATTCCTGATCTATACCCTGATCGGAATGAGTCCCAGATTAGC AAAATCCCTAGGCAAAAAGTAAATCGCAAGACAGAAGTAATTTCTGGAGTGAAATGTTTTAGTAATGACC AAGGTGTTCATTGCTCAGAAAAGGATAAGTCTTTGTTACTACAAAAGGATAAAGACTTCCCAGGAACTTT AAAAGACTTAAGTGAGTTTGATACGCCTGCTTTTTGTAACAAAGATAGTGCAAAGTCGTGTGATTATAAG TCTGAAATGCTCTTGGGGTTGAAAAAACATGACCCTAATATGCAACCTGCTTGTCAAGATGATTCAAAAG CAGGTAAGAAACTTAGACAAAAGGTAAATCGAAAAACAGAAATAATTTCTAAAATCACCCAAATACATGA AAATGATAGAGGAAGTACACATGACTCATTAAATAAGAAGCTCTGTCAGAAGGTTAATATATCAAAAATC ATTTCTCAAATGAACCAAATATATGAGACTATTAATGAAGATGGAAATGGCTTTAAAAGCTCTATCAAAG ATTGCGAAGATATTAAAAGTTGTGACTTTGGGGAAATCAACAGTAATAAAAAGGAAAATTATGATCCAAT TCAAGATCCTTGCACACTGGTTAAAAAAACAAAGAGAAAGGGATCATGTAAAGCAGGGAGCAGTTTGGCA GGAGCTAAGAACAGGTGTGGTTTGCAGTTAACAGACTCTTCCCAGGTACAGTCTGTCCCCTTAGACTCTG GCTTAAGACACCATCCAAACGAAGCAGATTCTGGTCCTGGAGAGCAGACTAACCTGCCAAAGATGCAGAA ACAAAGCGCTGGGAGGTCACTGGGAGATGCTTTCTCTGTGAGTCTGGGAAAAGAAGGAAGCCGCCCAGCC AAAGCAGTTAGTAAAATGACACCCAAATCAAAGAAGAGAAAGCTCCCTCTCGGTTGTTCTCCTGAAACCC ACGGGACGGTGGAGATAACACCCAACACTGACCTCGCTAAGGCTGTTGACTCCCAACAGACTGAGAAGGA GAACTATTTGGAGAAGGAGAAAATTGCCAAGAGGAAGCCAGATTTTTGTACAAAGGTGTTGAAACCTTTA TCTGAGACATGTTCATCTAACATAAAGAATTCTTCCTTGGACAGTATGTGTAAGAGTTCGCTACCTTTGA GTATTTCTTCTAGAAAAACCCTGATGCTGGAAGAAAGTTCTTCCCTGGAGAGTACATGCATCTTTCAAGT AGGTGATGCCGCTCATGAGAAGATAACGACAGGCACACGTAATCCCCACCACAGGACACAGAAGTCGACA CCGGGTAGCAGAACGTCCCTGGTCTTGGTGGATACCAGTTCTGTTTCAGATACCAACCCTGCTAACCCCG AGAATGAGTCAGAAGGGCAGTCTTCACACCCAATGAGAAGGAAAAGACAGTGCGTCCCTCTCAACCTGAC AGAGCCAAGCCTTAGAAGCAAGATGAGGAGATAAAGTCGAAGTGAAGTGGACATGGATCCTGGTTTTCCC GAGTTCTCAAAGCACGGGTCAGAACAGAAGCACAAGAAGAACATGAAACAAAGACGGATTCAGTTGTTTG AGTTTTGTACGTGAAGTGTTTGGTTTTGGTTTTGGGGTTTTTTTGGCCTTTCTTAGATTTTGCAAGATCT ATGTGCATGAAATAGCTATATCTTAGATAATAAACTTTTTTTTAAAAACATTGGTCATCACATAACTTGT TGATTTAAATTTCTATCAAATTTATATAACCAAGCAACTATTCTTATTAACCAAATAAAATGTATCATTG CATTGGTTATCTTGTATATCAGGCTATCTTGTCTTCCTAACATTGAGGATGCCATCTTTACTGCTAAAAC ACAGGAACACACCACCCTGCCTGTGCAGTGGAGGTTGGAGGCCTTGTTAACAGAACATTTACTTTGTGGG GAGGGTTGCCTTTCTGACATCTGTTCATGCTCCAGGAATACTGACCTCCTGGAATCCCTCTGACAGTATA GTTTTTGTTCCTTTTTTTTTTTTTCTACCTTTTATGAGATTTCCCTTTATTTCTCTCTCCCATGCAAATT ACTTCATATTTTATACCAAGTATCTTTCTTTTACTATTGGTAGAGTTTGATCACACTTAAAAAAAACGTT TTCAGGTTATAAAGTACCCATGGTTTTAATTTAAAAAATGTTTAGGTTGTGTGCGTGCACACATTCATGC TACAGAGCATGTGGAGAGTCGGCTCTGCTTTCCTCATGAGGACCTGCTAGAGGGTGGAACTCAAGTCACC TGCTTTGCTTTTTGCCCACTGAGCGGTTTTTGTGAGCCCTACATTTAAATTTCAGACTAATACAGAGTTT GTTGTATGATGTGAAGTATACCTGTAATATTTTACTATTTCTCAAGTGATTTATTGACTTAAGAGCTCGT ATCTATCTACTGATTTGAAGTTTTTAGCGTTGTGTTGTATTCCCATGTGTGTCAATTGTTGATTTTCCTG ACTCCTATTCACTTATGTATCATTTTATCATTAATTCTCTTTATTTATTTTTTAAATACAAATTAAAAAA AGTTTTAAAGATTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_199007
• Insert Size: 3495 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052742, AAH52742
• RefSeq Size: 4863 bp
• RefSeq ORF: 3495 bp
• Locus ID: 68549
• UniProt ID: Q7TSY8
• Cytogenetics: 1 28.86 cM
• Gene Summary: Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation. Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments. Involved in centromeric enrichment of AUKRB in prometaphase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).