Spsb4 (NM_145134) Mouse Untagged Clone

CAT#: MC205164

Spsb4 (untagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 4 (Spsb4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spsb4
• Synonyms: D030068E18Rik; SSB-4; Ssb4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055705
  CCCAGGCAGGCGCTCGCCCTCTCTTTTATGGAGCTTGGGTCCCTGCCACAGCCCAGCAGAGGCTGTGGAG GTCATCCATCAAGAAGCCTGGTTCCCAGCCTCAGGGTCCATCCTTGGATACCAGGATTGGAGGCATCTGC GAGGTAGCAGTGGCCAGCAGTGGTCCCTTCCCCATAGTGAACCATGGGTCAGAAGCTCTCTGGGAGCCTC AAGTCTGTGGAGGTGCGAGAGCCAGCGCTTCGGCCAGCTAAGCGGGAGCTCCGCGGCTTAGAACCTGGGC GACCGGCACGTCTAGACCAGCTGCTGGACATGCCTGCGGCAGGGTTGGCAGTACAGCTGAGACACGCGTG GAACCCCGAGGACCGCTCCCTCAATGTCTTTGTCAAGGATGACGACCGCCTCACCTTCCACCGGCACCCA GTAGCCCAGAGCACAGATGGCATCCGTGGCAAGGTGGGCCATGCCCGGGGTCTGCACGCATGGCAGATCC ATTGGCCCGCTAGGCAGCGGGGCACCCACGCTGTGGTGGGCGTGGCCACGGCCCGAGCCCCTTTGCACTC TGTGGGTTACACCGCACTGGTGGGCAGTGACTCCGAGTCGTGGGGCTGGGACCTGGGTCGCAGCCGCCTC TACCACGACGGCAAGAACCGGCCTGGGGTGGCATACCCAGCCTTCCTGGGGCCAGATGAAGCCTTTGCTC TGCCAGATTCCTTATTGGTCGTACTGGACATGGACGAGGGCACGCTCAGCTTCATCGTGGATGGCCAGTA CCTGGGCGTGGCCTTCCGTGGCCTCAAGGGCAAGAAACTATACCCGGTGGTGAGTGCTGTGTGGGGTCAC TGCGAAGTCACCATGCGCTACATCAACGGCCTTGACCCTGAGCCTCTGCCGCTGATGGACCTGTGCCGGC GATCCATCCGCTCAGCCCTGGGCCGACAGCGCCTGCGTGACATCGGCTCCCTGCCCCTGCCTCAGTCTCT GAAAAACTACCTGCAGTACCAGTGAGCGGCGCTGAGGATCGGCTGTCATCTGTGGTCACACAGCTCCAGA GCGTCAGGACACATCAGTGACTTGGAAAAGGAATGCCTGTTGCCATTGCTGCCACCGACCAGGACCAGGA GGCCACCTCGTGAACCAGAGTGTAGAGACCATTATCTTCCTCAAGAGAATGAATAAAACATTGGGCAGGA GACGGCATCCAGTTAGTTCCCCACTGCAGACAGACCCAGGAAAATTGGCCAGTGGAGAGAAAATGGGTTC TGAATAATGAATGACAGAAGCCAGCCCTTGATGGTCTCCTCCACGCCTGCCCCTGGCCAGGGCAGAGAGA CTGTCCTCCATTTTAAGACTTGGTACCACTTTGAGAGATGAGTCCAGGGTCCCACAGCAGGGCAAAAGCA CCTGGCCTTACCTCTCTGAGCCGGTTTCCTCATCCCCTCCTGCCCTCTTCCGTCATGGATAAAGTTCTTT GGCCATTCCCCTCCCCACTTTATTCAGGGACATGAATCTGATGACTTGCCCTGGGGACAGAGTTTCTTCT TGGGTATGATCATTGTAAGTAGTGTTTGCATTTTTTTTTTTTGAAAGAGGAGTGTGGTGAAGGTGGCTTT CTCCCAAAGGTGCTAAGGAAGATGGGGCCCTCGTGACACCAAGTCTGAGACACCCTGAGTATCCAGGGCT CTGGCAGCCATCATTCAGGGTGAAGCCTGGCTGTCACAAAGCTTTCTTTGAGCAAATTATTTTTTCACTG TAGGAGACTTCCAGTTTGTTTCTCGTTTCCAATATGTGTGTGATGCACCGTTTATTTATCAGCTGGGGCC CCGCGGTGTGGCCTTGGGACTGCAAAGGTGGGTATAACAGTCTCTGCAGGACCTGAGACCTATCCTCTCA CAGAATGTTGCTGCTGCTGCTGCTGCTGCCGCCAGCGCCACCATTTGGCATCTTTTGTGTGTTCCAAGAT ACCCTCCACCCAGACTCAGTTTTAACTGGAAATCATCACAGAGTAGGATACAGAGCCAGACAGTGCAGCC TACACTGTAATGTGAATGTGACCTACAAGCAAGGAACATTTCTAATAAAAGTTTGTCATTCTGTCCTTCA AAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_145134
• Insert Size: 822 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055705, AAH55705
• RefSeq Size: 2125 bp
• RefSeq ORF: 822 bp
• Locus ID: 211949
• UniProt ID: Q8R5B6
• Cytogenetics: 9 E3.3
• Gene Summary: Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (By similarity). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity). Diminishes EphB2-dependent cell repulsive responses by mediating the ubiquitination and degradation of the EphB2/CTF2 (By similarity). Regulates cellular clock function by mediating ubiquitination and proteasomal degradation of the circadian transcriptional repressor NR1D1 (By similarity).[UniProtKB/Swiss-Prot Function]