Sema3a (NM_009152) Mouse Untagged Clone

CAT#: MC205153

Sema3a (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema3a
• Synonyms: coll-1; Hsema-I; SEMA1; Semad; SemD
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057588
  ACAGTTGTGCCTCATTATCCGGTGCCTGGCTCGATTTTTTTCTTTCTTTTTTCTTTTTTTCTTTTCTTTC TTCCTTTTTTTTTTTTTTCTTTTTTTGAGGGTTTGAAGTTTCTGTGATTCCGTGACTGTTACAGAAGAGA CGTTAGTGTTGCCATGAGGTCTTGATTGTCTGCATTTATGAATGAAACTGACCTAAATCACCTGTTACCT CCAGTTTCCAGATTGTTTGAACTTCTCTGGCCGCACAATACAGGAAGGAAGGCTGCCGCAGCTCAGGGAC CTCCAGCGTCTGCAGCATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTGTATTACTTACAGCC AGAGCAAACTATGCAAACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAAT CCAACAATGTGATCACTTTTAATGGCTTGGCCAACAGCTCCAGTTACCACACCTTCCTTCTGGATGAAGA ACGGAGTAGACTATATGTTGGAGCAAAAGATCATATATTTTCATTCAACTTGGTGAACATTAAAGATTTT CAAAAGATTGTGTGGCCAGTATCTTACACAAGGAGAGATGAATGCAAATGGGCTGGAAAAGATATCCTGA AAGAATGTGCCAATTTCATCAAGGTCCTGGAGGCTTATAATCAGACTCACTTGTATGCCTGTGGAACTGG GGCTTTCCATCCAATCTGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAGCTGCAG GACTCACATTTTGAAAACGGTCGTGGGAAGAGCCCTTATGATCCCAAACTACTGACTGCCTCTCTTCTAA TAGACGGTGAGTTGTACTCTGGAACTGCTGCGGACTTCATGGGACGGGACTTCGCTATCTTCAGAACACT GGGGCACCATCACCCCATCAGGACGGAGCAGCATGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGT GCCCATCTCATCCCAGAGAGTGACAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAA TAGACGGAGAACACTCTGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGTGG ACACAGAAGTCTTGTGAATAAATGGACAACATTCCTAAAAGCACGCCTGATTTGCTCTGTGCCCGGTCCC AATGGCATTGACACCCATTTTGATGAATTGCAGGATGTATTCCTAATGAACTCTAAAGATCCTAAAAATC CGATCGTCTATGGAGTGTTCACAACATCAAGCAACATCTTTAAGGGATCTGCTGTGTGCATGTACAGCAT GAGTGATGTAAGAAGGGTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTGCCT TACCAAGGAAGAGTCCCTTATCCACGGCCAGGAACTTGTCCCAGTAAAACATTTGGCGGATTTGACTCCA CAAAGGACCTTCCTGATGATGTCATAACTTTTGCAAGAAGTCATCCAGCCATGTACAACCCAGTGTTTCC TATAAATAATCGCCCGATCATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGA GTGGATGCAGAAGATGGCCAGTATGATGTTATGTTCATCGGAACAGATGTTGGAACCGTTCTTAAAGTGG TTTCAGTCCCCAAGGAGACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACCGTCTTCCGGGA ACCAACAACTATTTCGGCAATGGAGCTTTCTACTAAACAGCAACAGCTGTACATTGGCTCAACTGCGGGA GTGGCACAGCTTCCTCTACACCGCTGTGACATCTATGGCAAAGCCTGTGCAGAATGCTGCCTCGCTCGGG ACCCTTACTGTGCCTGGGATGGGTCCTCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACG ACAAGATATAAGGAATGGAGACCCACTGACTCACTGCTCTGACTTGCAGCACCATGATAATCATCATGGG CCCAGCCTTGAAGAGAGAATCATCTATGGAGTGGAAAACAGTAGTACATTCTTGGAATGCAGTCCGAAGT CACAGAGAGCCTTGGTATATTGGCAATTTCAGAGGAGAAATGAAGATCGAAAAGAGGAGATCAGAATGGG TGATCATATCATCAGGACAGAACAAGGGCTCCTGCTCCGTAGCCTGCAGAAGAAGGATTCAGGCAATTAC CTGTGTCACGCTGTGGAACACGGATTCATGCAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAG AACATTTGGAAGAACTTCTTCATAAAGATGACGATGGAGATGGCTCTAAGATAAAAGAAATGTCGAGCAG CATGACGCCCAGCCAGAAAGTCTGGTACCGAGACTTCATGCAGCTCATTAACCACCCCAACCTGAACACG ATGGATGAGTTCTGTGAACAAGTGTGGAAAAGGGACCGAAAGCAACGCCGACAAAGGCCGGGGCACTCTC AAGGGAGCAGCAACAAGTGGAAGCACATGCAAGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTT TGAGCGGGCACCCAGAAGTGTCTGAGCTGCGCCACCTCCCAAAACCTCAAACAAGTACAAACTTGCTTAG ATAATAACTGGAAAAAATGCAATACACATGAACATTTCATGGCATTATGTGGATGTTTACAATGATGGGA AGTTCAACCGGGTTCCACCAATTTAAGTCCGTGAGGAACTTTCCCAGCAGGCTTTCTTCAGTATACCAAC GCTTGACAGAGATCACAGGTGAGCATAGATGTTCACGTCCGCTGACTTAGTGTTTCCTCTGAAAGTTCAT TTTGCTTCCTTCTTTGCCTGAGAAATAAAAATGTCATTTGCCATCGAAAGGAGAAAAACTGCATCAGCAA AGCCATTTTATTGAAGCAAGAGTTGAAAATAAACTGCATGGATTTAGTAAGCAGATGAATATTCCAAAAC GTGATTGGATTCAAGGATGTTTTGTCTACCAGCACTCGTGTTGTATGTACTGGAGAAGTAAAATAAGGAG AATGACACTGAGTGAAATAGTCTATGGAGATATAAAAACACAAACCACCCATCATCCAGAGTAATGGAGT ACATTGATCTACTACTGATGTCTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAGTTATAATTTA AATGTACCATGGCAAATATGCAGTAAAGATTAGCTGTTTCCTAAGCTAGAGTAGGTCTTGTCTTGCAGTT ACCATGCTATATCGTTTGTTTAAAAATTCCAACTGTGTGCTGCTTTTTTACATTGTGTTTTCAGTTCTGT AAGAGGCAGTATATCACTTTAGGTCCCTACTCACTGTACTATTACTTACTGACACCAAGAATTATGGTCT TCCCCTTTGAAGAAACCAAACAGATAGAAGGTTATCACCCTGACTTGCAAAGGTATTATCTAAGTTAACT CTCACATACATAATGTTTTCAGTCAACCAAAAATTAAGACAGCACAAAGAGAAGAAAACAGTCATTAGAG GCATCCTGAAGGATCAAGAGCAAAATTAAATTCATGTGTCTCATGAAAAGAACATTGTCTTAACAGAGTG TTGATAAAATGTCTTTCCCTCTTGGCTTGAATATTTGCATGATGTGTGAGGCATAGGACTCGCTCATGAT TCCACATCCCACCAAAGATTCTATTTTCAGTCACTTAATGATTTCCAAAGCAGAGAGAAAGAGCGGGGGT GGGGGGAATCAGTGACATTCAGGACATGTTCTAACAACAAAATGACACATTTCTCAACTATTTCTTTGTG AATCAACTAAATTTTACCCACCCTGTATGATACAATTATTTGTCTGAACAACTTATAAGTGTTTTATCTC AATGAGAGTGAAACTATACACTTCACCTAGAAAGTGCAAAATTCTTTGTTTTATGAATAAAATATAAGAT AACACAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_009152
• Insert Size: 2319 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057588, AAH57588
• RefSeq Size: 4013 bp
• RefSeq ORF: 2319 bp
• Locus ID: 20346
• UniProt ID: O08665
• Cytogenetics: 5 A1
• Gene Summary: Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.