Cdyl (NM_009881) Mouse Untagged Clone

CAT#: MC205145

Cdyl (untagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdyl
• Synonyms: AI325931
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055103
  GAGGACATCTGTTCCTACCTAAGAGCACTCACCTGAGATGCTCAAAGGTCCAGAAGAAACACTTCTCGGG TGACAAAGCAGGTGGTGACCAGAGAACAGAGGCCCCCCAAAAATTTTATGGCATTCAAGGCAAAGCACAG CCAACCCGGAGGGAAAGCAAGAGTCCAGCCTGGAAATACATAGCCCAACCCGAAGGTTATCTCTGAAGGA AAACAATGGGCATAGGCAATAGCCAGCCTAATTCACAGGAAGCCCAGCTCTGCACACTTCCAGAGAAAGC TGAACAACCTACTGATGATAACACCTGCCAGCAAAATAATGTGGTTCCTGCAACAGTCTCAGAACCCGAT CAAGCGTCCCCTGCAATTCAAGACGCGGAGACTCAGGTGGAAAGTATCGTTGACAAAAGGAAAAACAAGA AAGGGAAGACAGAATATCTGGTGCGGTGGAAAGGCTATGACAGTGAGGATGACACGTGGGAGCCTGAGCA GCACCTGGTGAACTGTGAGGAATACATCCATGACTTCAACCGGCGCCACAACGAGAGGCAAAAGGAAGGT AGCCTGGCTCGTGCCAGCAGAGCCTCCCCCAGCAACGCCCGGAAGCAGATTTCCAGGTCCACCCACAGCA CTCTCTCCAAGACCAACTCCAAAGCACTTGTGGTAGGCAAAGATCATGAGTCCAAAAGCAGCCAGCTGTT GGCTGCCAGCCAGAAGTTCAGGAAAAACCCAGCCCCATCTCTTGCAAACCGCAAGAACATGGACCTCGCC AAGTCAGGGATCAAAATTCTCGTGCCTAAGAGCCCCGTTAAGGGCAGGACCTCGGTTGATGGCTTTCAGG GGGAGAGCCCCGAGAAGCTGGACCCTGTGGATCAGGGTGCCGAGGACACTGTAGCCCCAGAGGTGACTGC AGAGAAGCCCACTGGGGCTTTGCTGGGCCCTGGTGCGGAGCGAGCCAGGATGGGGAGCAGGCCCCGAATA CATCCACTAGTGCCTCAGGTTTCTGGCCCCGTGACTGCTGCCATGGCCACAGGCTTAGCTGTTAATGGAA AAGGTACATCTCCATTCATGGATGCGCTAGCAGCCAACGGAACAGTCACCATACAGACATCCGTAACAGG AGTGACAGCCGGGAAAAGGAAATTTATTGACGACAGAAGAGACCAACCTTTTGACAAGCGGTTGCGTTTC AGTGTGAGGCAGACAGAGAGTGCCTACAGATACAGAGATATTGTCGTCAGGAAGCAAGATGGCTTCACCC ACATCTTGTTATCCACAAAATCGTCAGAGAATAACTCACTAAACCCAGAGGTGATGAAAGAAGTGCAGAG CGCCCTGAGCACAGCTGCAGCCGACGACAGCAAGCTGGTTCTGCTCAGCGCCGTGGGCAGCGTCTTCTGC TGTGGTCTGGACTTTATTTATTTTATTCGGCGCCTCACAGATGACCGAAAGAGAGAAAGCACTAAAATGG CAGACGCTATCAGAAACTTCGTGAATACTTTCATTCAGTTTAAGAAGCCTATTATTGTAGCTGTTAATGG CCCAGCCATTGGACTAGGAGCATCCATATTGCCTCTTTGTGATGTGGTTTGGGCTAACGAAAAGGCTTGG TTTCAAACACCCTATACCACCTTCGGACAGAGTCCAGATGGCTGCTCTACCGTTATGTTTCCCAAGATTA TGGGAGGAGCATCTGCGAATGAAATGCTGTTCAGTGGGCGGAAGTTGACGGCACAGGAGGCCTGTGGCAA GGGTCTGGTCTCCCAGGTGTTTTGGCCAGGAACCTTCACACAGGAAGTCATGGTTCGAATCAAGGAGCTG GCTTCATGTAACCCAGTTGTCCTGGAGGAATCCAAAGCCCTGGTGCGCTGCAATATGAAGATGGAGCTAG AGCAGGCCAATGAGAGAGAATGTGAAGTGCTGAAGAAGATCTGGGGCTCCGCCCAGGGCATGGACTCCAT GTTAAAGTACTTACAGAGGAAAATCGATGAGTTCTGATGGGCAGGCTGAGCAGGACATCGGTGGCTCCCA CTTGCTACGTCGTCCTGCAGTGGCTCGTGCTTGGAGGCAGAACTGGAAACATCCGAGCTATTTATTGCCG CGGAGTTTTTAAGTACTGTAACTTTAAAATAACTACAAAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: SfiI-SfiI
• ACCN: NM_009881
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055103, AAH55103
• RefSeq Size: 2174 bp
• RefSeq ORF: 1782 bp
• Locus ID: 12593
• UniProt ID: Q9WTK2
• Cytogenetics: 13 14.39 cM
• Gene Summary: Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:12947414). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:12947414). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (By similarity). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (By similarity). Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (PubMed:24144980). Required for neuronal migration during brain development by repressing expression of RHOA (PubMed:28076783). In addition to act as a chromatin reader, acts as a hydro-lyase (By similarity). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (By similarity). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (PubMed:28803779). May have histone acetyltransferase activity; such activity is however unsure in vivo (PubMed:12072557).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).