Plk3 (NM_013807) Mouse Untagged Clone

CAT#: MC205095

Plk3 (untagged) - Mouse polo-like kinase 3 (Drosophila) (Plk3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plk3
• Synonyms: Cn; Cnk; Fnk; PLK-3; PRK
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC063051
  AGCGTAGAGAAACCAGGCAGCGCCACGCGCGGCCGGGGCCGGGCGGAACCGAGAACCAGCTCGGCAGGCG CTGCGCCGCGCCGTCCGCATGGAGCCCGCCGCCGGCTTCTTGTCTCCGCGCCCCTTCCCGCGCGCGGCTG CCCCGTCCGCGCCCCCCGCCGGCCCGGGGCCGCCTGCCAATGCCTCCCCGCGATCGGAACCTGAGGTGCT GGCCGGGCCACGGGCGCCGGATCCTCCTGGTCGCCTCATCACCGACCCTCTCAGCGGCCGTACCTACACC AAGGGTCGCTTGTTGGGCAAGGGGGGCTTCGCACGCTGCTATGAAGCCACTGACACCGAGTCTGGTATAG CCTACGCGGTCAAAGTCATCCCACAGAGTCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGA GATAGAGTTGCACCGAGACCTACAGCACCGCCATATCGTTCGCTTTTCACATCATTTCGAGGATGCTGAC AACATATACATTTTCCTGGAGCTCTGTAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGACACACCC TGTTGGAACCAGAGGTTCGCTACTACCTGCGCCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGAGGGG TATCTTACACAGAGATCTCAAGCTGGGAAATTTTTTCATCACGGATAACATGGAACTGAAGGTGGGGGAT TTTGGGCTGGCAGCTCGATTAGAGCCCCCAGAGCAGAGGAAAAAGACCATCTGTGGCACTCCCAACTACG TGGCTCCAGAAGTGCTGCTGAGACAGGGCCATGGCCCCGAGGCAGATGTGTGGTCGCTTGGTTGTGTCAT GTACACGCTCCTGTGTGGGAGCCCCCCATTTGAGACTGCTGACCTGAAGGAGACATACCGCTGCATCAAG CAGGTTCATTACACGCTACCTGCCAGCCTCTCCCTGCCTGCCCGGCAGCTCCTGGCTGCCATCCTCCGAG CTTCACCCAGAGACCGCCCTTCCATTGAACAGATTTTACGCCATGACTTCTTTACGAAGGGCTACACCCC TGATCGGCTCCCGGTCAGCAGCTGTGTGACAGTCCCAGATCTGACACCCCCCAACCCTGCGAGGAGTCTG TTTGCCAAAGTTACCAAGAGCCTGTTTGGCAGGAAAAAGAACAAGAATAAGAACCATTCTGAGGACCAGG ATAATGTCTCCTGCTTGGTGAGTGGCCTCATGCGCACATCCATCGGTCATCCAGATGTCAGGCCGGAGGC TCCTGTAGTTTCAGGCCAGGCCCCTGCCAGCTTGGTAGAGACAGCTGCCGAGGACAGCTCGCCCCGTGGG ACACTGGCCAGCAGTGGAGATGGGTTTGAAGAAGGTTTGACTGTGGCCACAGTGGTAGAGTCTGCCCTCT GTGCACTGAGAAACTGTGTGGCCTTCATGCCCCCAGCCGAACAGAACCCAGCCCCGCTGGCACAGCCAGA GCCTCTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAACAAGTTTGGCTTTGGCTATCAGCTGTCCAGC CGCCGTGTAGCCGTGCTCTTCAATGATGGCACACACATGGCCCTGTCAGCCAATAGGAAGACGGTGCACT ACAACCCCACCAGCACGAAGCACTTCTCCTTCTCCATGGGTTCTGTGCCCCGAGCTCTGCAGCCTCAGCT GGGCATCCTGCGGTATTTTGCGTCCTACATGGAACAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTG GAAGAGGCGGAGGTGCCTGCCCCACCCTTGCTGCTGCAGTGGGTCAAGACTGACCAGGCCCTCCTCATGC TGTTCAGTGATGGCACTGTCCAGGTGAACTTCTACGGGGACCACACGAAGCTGATCCTCAGTGGCTGGGA GCCTCTCCTTGTGACCTTCGTGGCCCGCAATCGCAGTGCTTGTACTTACCTCGCTTCCCACCTCCGGCAG CTGGGCTGCTCCCCAGACTTGCGGCAGCGACTGCGCTACGCTCTGCGCCTGCTCAGGGACCAAAGCCCTG CTTAGGTTCCAACTCGGAGAGCGGGCTGTGATCCAGATCCTGGGCCTGAGCCTGAAAGGCTTCAGGGCCT TGCCTTTGTGGCCCTTCCTGTCCCTTTGGTGCCTCACTGGGGGCTCCTGGCTGAATCCCCCCAGGGAATC AGGGACCAGCTTTACTACAGTCGGGGATGGCCTGCCCTCCCTGAATCTTACCCTTCTCTCTGAGATAAGC CTGAGCCTTAGCTCCCAGCTAGGGGGCATTATTTATGGACCACTTTTATTTATTAACAGACATTTATTTA TTGGGATGTGAGCCCCAGGGGGGCCTCCTCCTGGGGTAATAAACCGTTTTTTCAGAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_013807
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063051, AAH63051
• RefSeq Size: 2474 bp
• RefSeq ORF: 1947 bp
• Locus ID: 12795
• Cytogenetics: 4 D1
• Gene Summary: This gene encodes a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal catalytic domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases have primarily been implicated in cell cycle regulation. In mouse, this protein that has been reported to localize to the nucleolus during interphase but is undetectable during mitosis, following nucleolus dissociation during prophase. The protein relocalizes to the nucleolus just prior to cytokinesis and peak levels are detected during G1 of interphase. This gene has been implicated in regulation of entry into S phase, with RNAi-induced depletion resulting in failure to re-enter the cell cycle. Mice deficient for this gene exhibit increased weight and tumor development at advanced age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).