Cars (NM_013742) Mouse Untagged Clone

CAT#: MC205092

Cars (untagged) - Mouse cysteinyl-tRNA synthetase (Cars), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cars
• Synonyms: CA3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058954
  GTCTGTGGCGGCGGCCGCGGGTCTTGATTCCCAGTGATGGCAGGTTCCTCCGCGGAGCAGGCTGCCGACT ACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCAGGGTGCAGGCCCTGGACCAACACCTCAGCACGCG TAGCTATATCCAGGGGTACTCCCTATCCCAGGCAGATGTGGACGTGTTCAGGCAGCTCTCGGCCCCTCCC GCTGACTCAAGGCTCTTCCACGTGGCTCGGTGGTTCAGGCACATAGAAGCGCTCCTGGGTGGCCCCCAAG GCAGAGACGAGCCCTGCAGGCTTCAAGCAAGTAAAGGCCGGCGAGTGCAGCCTCAGTGGTCCCCCCCAGC AGGGACTGAGCCCTGCAGGCTCCGCCTCTATAATAGCCTCACCCGGAACAAGGATGTATTTATACCTCAA GATGGGAAGAAGGTGACGTGGTACTGTTGCGGGCCGACTGTCTATGATGCGTCTCACATGGGACATGCCA GGTCCTACATCTCCTTCGATATCCTGAGGAGGGTGCTGAGGGATTACTTCCAGTATGATGTCTTTTACTG CATGAACATCACAGACATTGATGATAAGATCATCAGGCGGGCACGGCAGAACTACCTGTTTGAGCAGTAT CGGGAGCAGAAACCCCCAGCCACCCAGCTCCTGAAGGATGTCCGTGATGCCATGAAGCCATTTTCAGTCA AGTTAAGTGAGACAACAGATCCCGACAAGAGGCAGATGCTGGAGCGGATCCAGAACTCTGTGAAACTTGC CACAGAACCACTGGAACAGGCTGTGCGATCCAGCCTCTCTGGAGAGGAAGTAGACAGCAAAGTACAGGTG TTGCTGGAGGAAGCAAAGGACTTGCTCTCTGACTGGCTGGATTCCACAGGTGGCAGTGAGGTGACTGACA ACTCGATTTTCTCCAAACTGCCCAAGTTCTGGGAAGAAGAGTTCCACAAAGACATGGAAGCCCTGAATGT TCTCCCTCCTGATGTCCTAACCCGTGTCAGTGAATATGTGCCAGAAATTGTGAACTTCGTCCAGAAGATT GTGGACAATGGTTATGGTTATGCTTCAAATGGATCTGTTTACTTCGACACAGCCAAGTTTGCTGCTAGTG AAAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGGGATCAGAAGGCACTTCAGGAAGGGGAAGG TGATCTGAGCATCTCTGCTGACCGCCTTAGTGAGAAACGCTCTCCCAATGACTTTGCCTTGTGGAAGGCC TCCAAGCCAGGCGAGCCATCCTGGCCTTGCCCCTGGGGAAAGGGTCGTCCCGGATGGCACATAGAGTGTT CTGCTATGGCAGGCACGCTCCTGGGAGCCTCAATGGACATTCATGGTGGAGGGTTTGACCTCCGCTTCCC CCACCATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAATGACTGCTGGGTCAGGTACTTCTTG CACACGGGCCACTTGACGATAGCAGGCTGCAAGATGTCCAAGTCACTGAAAAACTTTATCACCATTAAAG ATGCCTTGAAGAAGCACTCAGCACGGCAGCTGCGACTGGCATTCCTCATGCACTCATGGAAAGACACACT GGACTATTCCAGCAACACTATGGAGTCTGCTCTTCAGTATGAAAAATTCATGAACGAGTTTTTCTTAAAT GTGAAAGACATCCTCCGAGCCCCTGTAGACATCACTGGCCAGTTTGAAAAGTGGGAAGCTGAAGAAGTGG AGCTAAATAAGAACTTCTATGGCAAGAAGACCGCAGTTCACGAAGCCTTGTGTGATAACATTGACACCCG GACTGTCATGGAAGAGATGCGGGCTTTGGTCAGTCAGTGCAACCTCTACATGGCAGCCAGGAAGGCTGAA CGGAGGAGGCCCAACCGGGCTCTGCTGGAGAACATTGCCATGTACCTCACCCACATGCTGAAGATCTTTG GGGCCATAGAGGAGGAGAGCCCCCTGGGGTTCCCAGTTGGTGGGCCTGGAACCAACCTGAACCTCGAGTC AACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCAGAGAGGGAGTACGAAAGATTGCCCGAGAGAAA AAAGTTCTTGAGGTTCTACAGCTCAGTGATGCCCTCCGGGATGACATCCTGCCTGAGCTTGGGGTCCGGT TTGAAGACCATGAAGGGCTGCCAACAGTGGTGAAGTTGGTGGACAGAGACACCTTACTGAAAGAGAAGGA AGGAAAGAAAAGGGCTGAAGAAGAGAAGAGGAGGAAGAAAGAGGAGGCGGCCAGAAAGAAACAGGAGCAA GAAGCAGCAAAGCTGGCCAAGATGAAGATACCACCCAGTGAGATGTTCCTGTCAGAAGTCAACAAGTATT CTAAATTTGATGAAAATGGTCTGCCTACCCACGACACCGAAGGCAAAGAGCTGAGCAAGGGCCAGGCCAA GAAGCTGAAGAAGCTCTTTGAAGCCCAGGAGAAACTGTACAAGGAGTATCTGCAAATGTTACAGAATGGC AGCCTCCAGTGATGCCACGGCAGCCCAGGAGGGGCGCACCTGCCAGCCTGGCTGCTATGGACTAGGCCAC AGCTACTGAGGCTCCGTGCCCTGATCATGTTTACAGCTGTTCTCACACCTGAATCAGGCAGAGTGGTAGC AGCTCTGGGACACTGATAATAAATTCTTCTGAACGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_013742
• Insert Size: 2496 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058954, AAH58954
• RefSeq Size: 2749 bp
• RefSeq ORF: 2496 bp
• Locus ID: 27267
• UniProt ID: Q9ER72
• Cytogenetics: 7 88.25 cM
• Gene Summary: Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).