4933405O20Rik (NM_172901) Mouse Untagged Clone

CAT#: MC204977

4933405O20Rik (untagged) - Mouse RIKEN cDNA 4933405O20 gene (4933405O20Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4933405O20Rik
• Synonyms: AV046776
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060958
  AGTAAAGATGTTGGCAGTAACCAGCTGTTCTATGAAAACAGTTCTCCAATATGCTGTGTTTCTTGGCCAT TCTAGGGAGGTAGTGTGTGAGCTTGTGACCTCCTTCAGAAGTTTCTGCTCACATTGCGCAGTGCCTCCTT CACCAAAGTATGGAGGAAGACACACGGTGGCTATGATTCCTGGAGATGGCATTGGGCCTGAGCTTATGGT TCATGTCAAGAAAATATTCAGATCAAATTGTGTGCCAGTGGACTTTGAAGAGGTGTGGGTCACCTCGACA TCTAATGAAGAGGAGATCAACAATGCTCTCATGGCCATCCGTCGGAACCGTGTCGCTCTGAAGGGCAATA TTGCAACCAACCACAATCTGCCTGCCCGTTACAAGTCTCACAACACCAAGTTTCGCACCATCCTTGACCT CTATGCCAGTGTCGTCCATTTCAAGACTTTTCCTGGTGTTATGACCCGACACAAAGACATAGACATCTTG GTTGTTCGGGAGAATACAGAGGGGGAGTACACTAACCTAGAGCATGAGAGCGTGAAAGGCGTGGTAGAAA GCCTTAAGATTGTGACAAAGACCAAGTCTGTGCGCATTGCTGATTATGCCTTCAAGTTGGCCCAAAAGAT GGGGCGGAAAAAGGTGACAGTTGTTCACAAAGCCAACATCATGAAACTGGGAGATGGGCTCTTCCTTCAG TGCTGTAAGGATGTGGCAGCTCACTATCCTCAGATCACCTTAGAGAGTATGATTATAGACAACACCACAA TGCAGTTGGTATCGAAGCCCCAACAGTTTGATGTCATGGTGATGCCCAATCTTTATGGCAACATTATCAA CAGTATCTGCACAGGACTGGTTGGAGGGTCAGGAATTGTCCCTGGAGCCAATTATGGTGATTCATATGCA ATATTTGAGATGGGTTCAAAGGAAATAGGTAAAGATTTGGCTCACAGAAATATTGCCAATCCTGTCGCCA TGCTGCTGACCAGCTGTATCATGCTGGACTACCTTGATCTCCAACCCTATGCCACTCACATCCGCTCTGC AGTCATGGCGTCCTTACAAAACAAAGCTGTCTGCACTCCAGACATTGGAGGTCAAGGGAACACTGCAAGC ACTGTAGAGTATATCTTACACCACATGAAAGAACAAACTAGTGGTTGTCATCCAAATTTCTTCCTGCAGT TCACTTGATTCACCCCCTCAGAGAGTCCAGCTAGCTTACTCAGCATGAATTGAGAGTGAACATTGCCAAT TCACTGGAAGGGAAGGTGGGGGGATCAATATTGGTTTTTCCATTAAATTTAAATTCAGAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: SfiI-SfiI
• ACCN: NM_172901
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060958, AAH60958
• RefSeq Size: 1348 bp
• RefSeq ORF: 1191 bp
• Locus ID: 243996
• UniProt ID: Q8BPC6
• Cytogenetics: 7 B4
• Gene Summary: Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.[UniProtKB/Swiss-Prot Function]