Rap2b (NM_028712) Mouse Untagged Clone

CAT#: MC204890

Rap2b (untagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rap2b
• Synonyms: 4021402C18Rik; AA408554
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC032168
  CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCGGACGCGTGGGCGCGCGGCGAGTAGGAAGCCCTC GCCCAGCGGAGGCTGCGGGAGACAGAGGAGAGAGAGTGATGGGTCGCGGAGCTGGACGCACGTTCCGCGG GGACTCATGCCACGCGTGTCTCAGCCGGACGCCCAATTAGCAGCCGCCTCTGCAACCAGCCGCCACCTCC TCCCGGCCCTCCCAGGCTGCCGGGGCGAAGAGCTCCAGCCGTTGTCTTGCTCCGGCTGCGCGCATTGTCC TCAGGGTCCTCCGACAGGGCTGCTGCGGGGCCCGGGACCCGCGCCCTAGGGACGCGCCCCCGCTGCCGGT CGGCCTGGCGCGGGGCTCTGCTAGTCTGTTGGCGAGCCCGTGCTACCGGGCTAGTCTCGCCGGGGTTTTT CCTGCGAAGTTGAGGAAGGGGAGAAGTCCACCCGTCCGCCCAGCCCAGCCTTCCCCGGCGCGCAGCCCCG ACGGGGCCGCGGCAGGCGCGACGAGGGCGCCGACGGAGCCATGAGAGAGTACAAAGTGGTGGTACTGGGC TCGGGCGGCGTGGGCAAGTCCGCGCTCACCGTGCAGTTCGTAACAGGTTCCTTCATCGAGAAGTACGACC CGACCATCGAGGACTTTTACCGCAAGGAGATCGAGGTGGACTCGTCGCCGTCGGTGCTGGAGATCTTGGA CACCGCGGGCACGGAGCAGTTCGCCTCAATGCGGGACCTGTACATCAAGAATGGCCAGGGCTTCATTCTC GTCTACAGCCTGGTCAACCAGCAGAGCTTCCAGGACATCAAGCCCATGCGGGACCAGATCATCCGCGTGA AGCGGTACGAGCGCGTACCCATGATCCTGGTAGGCAACAAGGTGGACTTGGAGGGTGAACGTGAGGTCTC CTATGGCGAGGGTAAGGCCCTGGCCGAGGAGTGGAGCTGCCCCTTCATGGAGACATCGGCCAAAAACAAA GCCTCAGTGGATGAGCTATTCGCAGAGATCGTGAGGCAGATGAACTACGCGGCACAGCCCAACGGCGACG AGGGCTGCTGCTCGGCCTGCGTGATCCTGTGAGGCGCCGTCTGCCGCCGGGCGCTGGCCACGCTCTGTGC ACAAAGCCAAACGCACCCGATTCTCTTAATGTGATTGTCTTCTTGCTTTGAGATTGGAGACGACTTTGTT GTCTTGGCTGGGATGTCCGAGGAACCTGGCTGACTTGTGTAGCCAGCATCCCCAGCCTTCAGCCAGGGTC TGAGAGGGTGTACGTTGCAGAGCATCTGAGACCCCGGTGGAAAAATGGCTCTATACAGCGTGTACGTTCC TCGTTGATTTTGGTTCATGCATATTTCCCCGTTTAAATAGCCATTAAGGCTCTGTATTGGCTGCTTGACA CCGGCAAGCAAAAGTTTCAAACCTGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028712
• Insert Size: 552 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032168, AAH32168
• RefSeq Size: 1440 bp
• RefSeq ORF: 552 bp
• Locus ID: 74012
• UniProt ID: P61226
• Cytogenetics: 3 E1
• Gene Summary: Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.[UniProtKB/Swiss-Prot Function]