Glrb (NM_010298) Mouse Untagged Clone

CAT#: MC204858

Glrb (untagged) - Mouse glycine receptor, beta subunit (Glrb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glrb
• Synonyms: AI853901; Glyrb; spa; spastic
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037605
  CCACGCGTCCGGGCGGTGGCGCGGGCGGCGGCGGGACTCGCTGGTCGCAGCTGCCTCTGCGCCCGCCACC TGCGCCGCCGTCACCTCGGCGGAGGGCTGAGGGGCGCGTGCGATTGAGCTGCTGAAACTTCCGTTTTCAA GATGAAGTTTTCGTTGGCAATCTCCTTTTTTATTTTAATGTCCTTGTTGTTTGAAGACGCTTGTGCTAAA GAAAAGTCTTCCAAGAAAGGGAAGGGGAAAAAGAAGCAGTACTTGTGCCCATCTCAGCAGTCACCCGAGG ACCTGGCCCGTGTGCCCCCCAACTCCACCAGCAATATCTTGAACAGGCTGCTGGTCAGTTATGATCCCAG GATCAGACCAAACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAATAGTTTTGGATCC ATTCAAGAGACAACAATGGACTATAGAGTTAACATTTTCTTGAGACAGAAATGGAATGACCCCAGACTCA AGCTACCTAGTGACTTCAGAGGCTCAGATGCACTGACAGTTGACCCCACCATGTATAAGTGCTTGTGGAA ACCTGACTTATTCTTTGCAAATGAAAAAAGTGCCAATTTTCATGATGTGACCCAAGAAAATATCCTGTTG TTTATCTTTCGGGATGGAGACGTCCTTGTGAGCATGAGGTTGTCTATTACACTTTCATGTCCTCTAGACT TAACTCTGTTTCCCATGGACACACAACGCTGCAAAATGCAACTTGAGAGCTTTGGATATACAACCGATGA TTTAAGATTCATCTGGCAGTCAGGAGATCCTGTTCAGTTGGAAAAAATTGCTTTACCTCAATTTGATATT AAAAAGGAGGATATCGAATATGGCAACTGTACAAAATACTATAAAGGCACTGGTTACTACACTTGTGTGG AGGTCATCTTCACCCTGAGGAGACAGGTTGGGTTCTACATGATGGGCGTATATGCACCAACCTTGCTGAT TGTGGTTCTCTCCTGGCTCTCTTTCTGGATCAACCCTGATGCTAGTGCTGCCAGAGTACCTCTGGGCATC TTCTCCGTGCTCAGTTTGGCCTCAGAGTGCACCACCCTCGCAGCCGAGCTTCCTAAAGTGTCTTATGTGA AGGCGCTGGATGTGTGGCTCATTGCCTGCCTGCTCTTCGGGTTTGCCTCCCTCGTGGAGTACGCTGTGGT CCAGGTGATGCTGAACAATCCCAAAAGGGTTGAAGCCGAGAAGGCCCGAATAGCTAAGGCTGAGCAAGCA GATGGGAAAGGTGGAAACGCAGCTAAGAAGAATACTGTAAACGGCACGGGGACCCCTGTTCATATCAGCA CTTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCCAAGTCTGATTTGAGATCCAATGACTT CAGCATTGTTGGAAGCTTACCAAGAGATTTTGAATTATCCAATTATGACTGCTATGGGAAACCCATCGAA GTCAACAATGGACTTGGGAAACCACAGGCAAAGAACAAGAAGCCTCCGCCTGCCAAGCCTGTCATCCCAA CAGCAGCCAAGCGCATCGACCTTTATGCAAGAGCATTATTTCCTTTCTGCTTCTTGTTCTTCAATGTTAT ATATTGGTCTATATATTTATGATAAACCATTTCCCTTTGTATGAAATACAGCACCATTTCATTGTGACCA ATGCAATTCATAATTGCTGATCTGTGGAAACTTTGCATTTTCCTAGCAATATATTGCATTTTGGATGCCA CTTGATTATAATAAAATACAGCACCTTACTTTTGAATGGCAGCATGACCCTGTAAAGTCTGTGCACCGAC TGTGAGATAGATAGATAGATGTATGTATATTGAGCATAGTGCTTTAAGAATCAATGACAAATATGCATAC TATGTAATCTACATAATGTGCTCTTCAGCTACTGTAACTCTGTAACTACAAATACTTCCAGTAATCCAGA TGAAAACATAATGTGCACGCTGACCCCTGTCATGTTCACTGGTCCATGTATGCAGTATCTTACATCCCCT TCCTTGTAAGAAAGAAAGCCATCTTATTAAATAATTTTCGATAGTATTATTGCAGATTAAGCAAGATTAT ATGGCATAGTGTTTAAAGTTTTTAAGAGAGGTAGATCAGTAATAGTTATGCAGGCTCCTTAAGAAATAAA ATCATAATTATACATTTATAAAGCAAGCTTATTTTTAAGTGTGCTTGTGTCGTCAAAATGCCAGATAATA GAACAGTAAGCATTTCTGAAGAAAAGAGAAATCTGGGCTAGTATAAATGCATAGCAAGTTCTGGCTTCTG GCAAAATACAAATAGTGATAGATGTATTAATTAAATATTTTGGATAAAATAAATCCTTTAATCCACCTTA AATCAAAGTCTATTTTCATGCCTTTCATTGGTTGGTATGGAGAGCATTAAGCATAAGCCTTAAAAAGAGT TGCACACATTTCAAACCTAAATAGTGTGGTGACATGTGAGTCCAGATTACTGTTAGACTCCAGAAATTGT TAGCATGAACTTGAAATGGAAAAATCATAATCACTGAAGGACTTAGGTTGTTTATTTTATTAAAATGTTT CTATATTTCAAGTAATATTCAAACTGTTTTTGGAATTTTTTTTATTTATTTGAATGTATTTGAATATGTT ACAAAACAATGATTTAGTTAATAATTTTTTTTATTTAACCTTTAGACTTCACCCTTTTCTTCTCGCTGAA AGTCTAGATATTGGGGCTGGCTGACTTCATCTTATTTCTATCTGAAAAGAACCCCTAAGCCTCTTCTTAT AAAGCCCCATTTTCTAACAGTGTCATAGTGTAACTGTTTATTTCTGTCTTAGCTAAAATTGGAGCTATTA GTAATTACTGTAATGTAAACAGACCATAAAGCCATTTTGCGTTATTTGGAGTGCTTTTGTTCTTGTTGAT AGTTTGTACAGCCAACTCATAACATAATGCATTAAAAGTATTATTTGTGCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010298
• Insert Size: 1491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037605, AAH37605
• RefSeq Size: 3008 bp
• RefSeq ORF: 1491 bp
• Locus ID: 14658
• UniProt ID: P48168
• Cytogenetics: 3 35.71 cM
• Gene Summary: This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. This gene is transcribed throughout the central nervous system of neonatal and adult mice. In humans, mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1.