Homer1 (NM_011982) Mouse Untagged Clone

CAT#: MC204803

Homer1 (untagged) - Mouse homer homolog 1 (Drosophila) (Homer1), transcript variant S, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Homer1
• Synonyms: homer-1; PSD-Zip45; SYN47; Ves-1; vesl-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC064041
  CTCTGCTCCGAGTCTTAGGAGGACGAACATTCAAAGGACAGATTCCAATGTGGTGTGCCGTGCACATCGG GAGCGGCTGGGGTTTGCACTTCGAGATTTCTTCTATATAATTTTTTTTTTTAAACGTAAGGGAGGCAGTA GCATTGCTGCCTGTAGGATTTTTATTCAAGTGCACGTCGCGTTGGGTTGCACGCTCCACCCCCAGGGACC TGGTGTGGTGAAATTTGAACCCACCGCCTTAGCCCAAAAAGGCCGAGTAACCTGGCTGCCTGAGTGTCGT GGAAGACGTGAGCGAAATGACCAGCGAACTCATTTTTTATCAGACTTGCTGAAGCTGGCTTTTGCGTTTT TTCTACACGTACGCTTAATTTTGTGGAATAGTTAAGTGCTATATTCTCCGCGCAACCTTTTCAAATTCCA AATGTTTGAACATTTTGGTGTCAGCGCGAGTGAAATCATTTTACCGACAAGAACTAACTGAATTGTCTGC CTTGTTGAGTTGCCTCCGGAAAAGATCTCGGGGGTGGAAAAGCAACTGCAAAATAACAGACGGAGAAAAT TCCTTGGAAGTTATTTCTGTAGCATAAGAGCAGAAACTTCAGAGCAAGTTTTCATTGGGCAAAATGGGGG AGCAACCTATCTTCAGCACTCGAGCTCATGTCTTCCAGATTGACCCGAACACAAAGAAGAACTGGGTACC CACCAGCAAGCATGCAGTTACTGTATCTTATTTTTATGACAGCACAAGAAATGTGTATAGGATAATCAGT TTAGATGGCTCAAAGGCAATAATAAATAGCACCATCACACCAAACATGACATTTACTAAAACATCTCAAA AGTTTGGCCAATGGGCTGATAGCCGGGCAAACACTGTTTATGGACTGGGATTCTCCTCTGAGCATCATCT TTCAAAATTCGCAGAAAAGTTTCAGGAATTTAAGGAAGCTGCTCGGCTTGCAAAGGAGAAGTCGCAGGAG AAGATGGAGCTGACCAGTACCCCTTCACAGGAATCAGCAGGAGGAGATCTTCAGTCTCCTTTGACACCAG AAAGTATCAATGGGACAGACGATGAGAGAACACCCGATGTGACACAGAACTCAGAGCCAAGGGCTGAGCC AACTCAGAATGCATTGCCATTTCCACATAGGTACACATTCAATTCAGCAATCATGATTAAGTAAGGTGGA TAAATATGGAAGTTCATTTGGTTTCAGAAACTCTTGAAGTTACAACCTTTGAGTGAAAAATCTCAGGTCA GACTCCTTTAATTTATTGTTCTTGGTTGCTCAAGTTGACTGAATTACTATATTTCCATTATCTATGTGGA AAAAGGAGCATTGAGCTAATTATAGGAGAAATTTTTTAAATGGAGAAAATATAATTCCTTTCTATCTATA TTTTAAAGATCCCTTTTGTTAACCCGTTTTCTGTTTTTATATATGTTATGTAAGATTTATAATGTGTAAT TAGAAACATAGAATTTCTACTCTGAAGGAAAGCTTTACCACAGGCCTACAGAGTTTTCACAGAAGACAGG GTACCAAGCACGAGCCTGTTAGCATTGATGGCAGATGCCAGCAGAAGGAAGGCTTGACTTCCTAATTCTG TATTCTAAAAGATACATCATGTTCTAAATGCATTTCAAACATTAGTTATTGGCCGTACCGTGGCATTACT GGACTGTAAACATGAATGTGAAATGGCACTATTGAAAATATTTTTTTAAAGCCCATCTACCTTAACACTA ATTTTTACCCTTATTTAAATGCTTTTTACTAAATAGTTTTAGGTAAAATTAAGAAAATAGGGGTTTTTTG ACTGCACATTTTTTTGAAGAACCAAGTTTTAGAAAATTATATTCTTTGACAGATTAAAAATTGCAAAGTG AGATATTTCAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_011982
• Insert Size: 561 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC064041, AAH64041
• RefSeq Size: 1922 bp
• RefSeq ORF: 561 bp
• Locus ID: 26556
• UniProt ID: Q9Z2Y3
• Cytogenetics: 13 C3
• Gene Summary: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 5 counteracts the effects by competing for binding sites. Isoform 3 regulates the trafficking and surface expression of GRM5. Isoform 5 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, isoform 2 and isoform 3 to regulate synaptic metabotropic glutamate function. Isoform 5, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development (By similarity). Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (S, also known as homer-1a or Vesl-1S) lacks several 3' exons but contains a 3' terminal exon that extends past a splice site that is used in variant L, resulting in a novel 3' coding region and 3' UTR, compared to variant L. The encoded isoform (S) has a shorter and distinct C-terminus, compared to isoform L.