Pigb (NM_018889) Mouse Untagged Clone

CAT#: MC204761

Pigb (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class B (Pigb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pigb
• Synonyms: Pig-b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052658
  AAGAGGTAATCCGTCCGCCTACAAGAAAGTGAGGGGCGGGCACCGTGGGCTGCAGGTAGCCGGGGAGGAT GGAGTCCCAGGCCGCCGACTATAACCCGGCCTCCCGCAATCTCCACGGCAGCTCGGGAGAAATGAAGCTG CGGAGGAGGAAGTCCAGGCAGTACGTGAGCGCGCAGGAGAAACGCTCTCCGCGCCGTGGTCTTCTTGGAG AAAACACTTACCTGGTCTTGTTTACCATCGCTCTGCGAATACTTAACTGCTTCTTAGTGCAGACAAGCTT TGTTCCTGATGAATACTGGCAGTCCCTCGAAGTTGCACACCGCATGGTTTTCAGTTATGGTTATTTGACC TGGGAATGGACGGAGCGATTGAGAGGCTACACTTACCCCCTCATCTTCGCCAGTATTTACAAGGTTCTGC ATCTTCTGGGGAAAGACAGTGTTCAATTCCTGATCTGGATTCCCAGACTTGGCCAAGCTCTTCTGTCTGC TGTAGCGGATATAAGGCTCTACTCCCTCCTGAAGCAGCTGGAAAACCAGGAAGTGGCACAATGGGTGTTC CTCTGCCAGCTGTGCTCCTGGTTCACATGGTACTGCTGCACACGGACCCTCACGAACACCATGGAGACCT CCCTCACTGCCCTCGCCCTTTTCTACTATCCTCTGGAAGGCTCGAGGTCCGTGAACAGCGTCAAGTACTC GCTCCTGGTGGCACTTGCTTGTGTGGTCCGCCCCACAGCTCTTATCCCATGGGTGCCGCTGCTCTTCAGA CATTTCTACCAAGAACAGAGAAAGCTCCATCTGACTCTGCATCATTTTTTACCTGTAGGATTTATAACCT TCAGTTTGTCTCTGATAATTGACCGTATCTTTTTTGGCCAATGGACGCTGGTCCAATTAAATTTCTTGAA ATTTAACGTGCTGCAGAACCTAGGGACGTTTTATGGCTCCCATCCATGGCACTGGTACCTCAGTCAAGGG TTCCCAGTTGTCCTGGGGACCCACTTACCTTTCTTCATTCACGGTTGCTTCCTAGCCCCAAGGAGGCTCC ACATACTGCTGCTGACCGTGCTGTGGACACTGCTGGTATACAGCATGTTGGGCCACAAAGAATTCAGGTT TATCTATCCAGTTTTACCATTCTGCATGGTGTTCTGTGGTTACTCACTAGCCCACCTGAAGACATGGAGG AAGGCAGCCCTGAGCTTCCTGCTCTTGTCAAATGTGCCACTGGCATTCTACACTGGCCTAGTTCATCAGA GAGGCACCCTGGATGTCATGAATCACGTTCAGAAAGTCTGCCCCCGAGGTCCTGACCCAGCTTCGGCCTC AGTATTCATAATGATGCCCTGCCACTCCACGCCTTACTACAGCCATGTTCACTGCCCACTGTCCATGCGG TTTCTCCAGTGTCCCCCAGACTTGACTGGAAAAACTCAGTATCTTGATGAAGCAGATATGTTCTACTTAA ACCCCTTAAGATGGTTACAACAAGAATTTCACAGCAATGCGTCACTCCCCACTCACTTGGTCACCTTCAA TGTTCTGGAGAAGGAGATCAATACCTTCCTCACCTCAGGGAACTACGAGAGGGCCGCTACATTCTTCCAC ACTCACTGGCCAGAGCGCCGAACTGGAAGTCACATACACGTCTACGAGCGAAGGTTACCCGGCAGGGTCA ACACAGGAGGGAACTGAACTGAAGCATAAGAACAACCAGCCTCGTCTCCATCGGCTTTATCCCTCAATGA GAGAAGAGACACAGCTCGCAGAGCAGTCTCCAACCTTCCCCCGTTCCAGGCAAGTGCAGTGAGCAGGCAC TGCTGTCTTCCCACTCTGCCTGCTGGCTCCTCCACTGCCTTACGCCAGGACCTGCACACAAGCGCTCTGC AGCGCTCAGCTCTAAGCACCTGACTTGACATCTCTGGGTTCACGTGCATCACTCTGCACTCTTCTCGCTT AGCCTTCTCTCTAACCAGCACAGATAGCATTGTCCCCTGCACTAGACCAATGGGGACTGGCTTCGAAGTG AGTAGAAATGGCCATGTACCTACCAACCCGTCCTCTCCAAATAAATGTCAACAGATAAAAAAAAAAAAAA AA
• Restriction Sites: RsrII-NotI
• ACCN: NM_018889
• Insert Size: 1629 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052658, AAH52658
• RefSeq Size: 2102 bp
• RefSeq ORF: 1629 bp
• Locus ID: 55981
• UniProt ID: Q9JJQ0
• Cytogenetics: 9 D
• Gene Summary: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly (By similarity).[UniProtKB/Swiss-Prot Function]