Pak3 (NM_008778) Mouse Untagged Clone

CAT#: MC204741

Pak3 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pak3
• Synonyms: PAK-3; Pak65alpha; Pak65beta; Stk4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053403
  AAGAGGTAATCCCCAGCCCATCTGAGTTCACTTTGCATCTCAATTTTGTTCTTCAACCTCTTTGATCCTC TGCCAGCTTTGAGTCATCTTCAGACGTGGAGCTGTGAAATCAGCTGCAACTGAAAATGTCTGACAGCTTG GATAACGAAGAAAAACCCCCAGCTCCCCCACTGAGGATGAACAGTAACAACCGAGACTCTTCAGCACTCA ACCACAGCTCCAAACCACTGCCCATGGCCCCGGAAGAGAAGAATAAGAAAGCCAGGCTTCGCTCTATCTT CCCAGGAGGAGGGGATAAAACCAATAAGAAGAAAGAGAAAGAGCGCCCAGAGATCTCTCTTCCTTCAGAC TTTGAGCATACGATTCATGTGGGTTTTGATGCAGTCACCGGGGAATTCACTGGAATTCCTGAGCAATGGG CACGACTACTCCAAACCTCCAACATAACAAAGCTGGAACAGAAGAAGAACCCACAAGCTGTTCTTGATGT TCTCAAGTTCTACGACTCCAAAGAAACGGTCAACAACCAGAAATATATGAGCTTTACGTCAGGAGATAAA AGTGCCCATGGATACATAGCAGCACATCAGTCGAATACCAAAACAGCTTCGGAACCTCCTTTGGCTCCTC CTGTATCTGAAGAAGAGGATGAAGAAGAGGAAGAGGAAGAAGATGACAATGAACCTCCGCCTGTCATTGC ACCAAGACCAGAGCATACAAAATCAATCTATACTCGCTCCGTGGTTGAGTCAATTGCTTCACCAGCTGCA CCAAATAAAGAAGATATCCCACCTTCTGCTGAGAATGCCAATTCCACCACTTTGTACAGGAATACAGATC GGCAAAGAAAAAAGTCCAAGATGACGGATGAGGAGATCCTAGAGAAGCTGAGAAGCATTGTGAGTGTTGG GGACCCAAAGAAGAAATATACGAGATTTGAAAAAATTGGCCAAGGGGCATCAGGAACTGTTTACACAGCA CTAGACATTGCGACTGGACAAGAGGTGGCCATAAAGCAAATGAACCTTCAACAGCAGCCCAAAAAGGAAT TAATTATTAATGAAATTCTTGTCATGAGGGAAAATAAGAACCCCAATATTGTCAATTATTTAGATAGCTA CTTAGTGGGTGATGAACTGTGGGTAGTCATGGAATACTTGGCTGGTGGCTCTTTGACTGATGTGGTCACA GAAACCTGTATGGATGAAGGACAGATAGCAGCTGTCTGTAGAGAGTGCCTCCAAGCTTTGGATTTCTTGC ACTCAAACCAAGTGATCCACAGAGACATAAAGAGCGACAACATTCTCCTCGGGATGGATGGTTCTGTTAA ACTGACTGATTTTGGGTTCTGTGCTCAAATCACTCCTGAGCAAAGTAAACGAAGCACTATGGTGGGAACT CCCTATTGGATGGCACCTGAAGTGGTAACTCGAAAAGCTTATGGTCCAAAAGTTGATATCTGGTCTCTGG GAATCATGGCCATTGAAATGGTGGAAGGTGAACCCCCTTACCTTAATGAAAATCCACTCAGGGCCTTATA TCTGATAGCCACTAATGGGACCCCAGAGCTCCAGAATCCTGAGAGACTGTCAGCTGTATTCCGTGACTTC TTAAATCGCTGTCTTGAGATGGATGTGGATAGAAGAGGATCTGCTAAGGAGCTTTTGCAGCATCCGTTTT TAAAATTAGCCAAGCCTCTGTCTAGTCTCACTCCTCTGATTATCGCCGCAAAGGAAGCAATTAAGAACAG TAGCCGTTAGAACTGCAAGCCTTACCCCTCACGGTCTCCAGGATGAGTAAGACTGAAATAAAACTCTGCT GCAGGATCCACAGAAGAAGAGACAGTCAAATGGAGTGGGGGCTCTTTAACTTTTAAGTGAATAGAAACTT CTTATAAACCTTTTTCCTACTCCCTCAGATTATGTAATTTATTTGTAAGCCTGAACTGCAGCCCACACAG GGCAGCAATGTCGAAGTAGCCATTAAGTGGCCACTTCCACCGTGAAGCGAAAGAGCCAGTAGTGAATCCC CTCATTTTGTGCATTTACTTTGAAGAAAAAAAGATTTCTCAAAGATGCACACTCCCTCTTCATAGTGCTG TGTTTGTTTTTAAGTTAGAGAGTAGTCCCTCTTTCATTCGAACCTCTTTCAAAATCCCTTACCCAACGTG ATGTTTTTTCACTTGCATTGTCATTAGATGTTCAGAAAAATAAAAGATGTCAAAATGTTTTTTTAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008778
• Insert Size: 1635 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053403, AAH53403
• RefSeq Size: 2270 bp
• RefSeq ORF: 1635 bp
• Locus ID: 18481
• UniProt ID: Q61036
• Cytogenetics: X F2
• Gene Summary: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity (PubMed:25851601). Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and lacks two consecutive in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform D), compared to isoform A. Both variants 4 and 5 encode the same protein (isoform D). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.