Sphk2 (NM_020011) Mouse Untagged Clone

CAT#: MC204704

Sphk2 (untagged) - Mouse sphingosine kinase 2 (Sphk2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sphk2
• Synonyms: C76851; Sk2; Spk2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053737
  CCAGAACCAAAGAGAAGTCAGCCTGAGGAAAGGGCTGGGACCCGGAGCCTCTCTGGCCTTTCCCCGTCCC TGCTCTAACACTCTCCAGGGGTAAAGGGACCGGAGAATCAGAGACATGATCGGAGCTTGCTGGACGAGTC GCGTGGTGACTCTCTGGCCGCACGCCGACCGCTTCTCGGTGGCTCGCGGAGGACCCGGTGGGCTGTGTGT CGGAGCCTCCGAAGTAGCTGGGATCACCGTCTTTCAACACTTGGCCTGGCTCTGCCATTTAAAGTTGTGA TCTTGGAGGCTGGTCCAGGAGCTGACCACAAGCCAAGAGCCTAGGAGTGCTTGGGACTGAACCAGGGTCA TGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACCC GGCCAACGGCCCACGGTTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGACTACGCCCAAAG CCAGAAGCCCGGCCCCGAGATGGTCTAGTCTCTCTGGATGAGGTCTCGGGCTGTGGCACCCTGCAGAGCC GTAGCCCCGAGGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGTCGAGGGGGCCGGCG CAGAGCTACGCGGACCTTCCGGGCGGATGGGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCGC TGGGCCACTGCCCTCACGTGTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAAT TGCTGCCCCGGAAGCCCAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGCG CTGTATGGACCACGTGGTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACGA CAGAACCATGCCCGTGAGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCTG GAGACGGGCTGCTTTACGAGGTGCTGAATGGGCTCCTTGATCGGCCAGACTGGGAGGATGCCGTGCGGAT GCCCATTGGTGTCCTCCCCTGTGGATCGGGCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTTT GAGCAGGTTGTCGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCTC TGGACCTGCTCTCTGTGACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGATT CTTGTCAGATGTGGACATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCACACTGGGTGCA GTGCTAGGCCTGGCCTCGTTGCATACCTACCGTGGACGCCTCTCCTACCTCCCCGCTACCACAGAACCAG CCTTGCCCATCCCAGGCCACAGTCTGCCTCGAGCCAAGTCAGAACTAGTCTTGGCTCCAGCCCCAGCCCC CGCCGCCACCCACTCGCCTCTACATCGATCTGTGTCTGACCTGCCTCTGCCCCTTCCCCAGCCTGCCTTG GTCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGAG ACTGGGGAGGAGCTGGGGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCAA AACAGCTCAGCTTTCACCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGTTCCTGCCTCCC ACCCACAGTGCCCCAGAAGCCTCTACCTGGGGCCCAGTGGACCACCTCCTCCCTCCCCTGGGCTCTCCAC TGCCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGGCATCTTGCCGAGCCACCTCTG CGCAGACCTGATGGCAGCCCCACATGCACGCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAGC GGCATCTCACGGGCTGCACTTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGCT GCCCCCATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCAC TGTAGATGGGGAGTTAGTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCGCCACGCTGCTC ACTGGGCCTGCAGGTCAAAAGCCGCAAGCCTGAACGAGCCTAAAAGCATGGCGAGTTGGTGGAACCAGCG CCCCATAGGCTAAGATCTATCATTTACAGGTAGAAGTGGGGCCCGCACTCAGAACTGTGAGGAGGGTGGA GAGTGGTCCTGACCCTCAGTTCCCAGAGGACCTAGAGGCTCGAGGGTGGGGCCTGCCTTTCTTGATGTCC AATGATGGGGCCTGGAATGTATGAGCTAGCAAGGCTTCTTCAGCTTATTGACCAGCCAGGGTTTCTTCTT GCCTACTCCGGTGCCTCTACTTGACTGGCCAATCAGCCCTTGAGGGGCAGGTTCCCCCAGGTGGTCCCCA GATTTGCACTAATGTTCCTCCCCTGGCCAGTTAGGGATGGGATGTTCTGTGTCTTGTGTGTCCCTCTCCC TAGTCTAAAAAGCAATTGAAAAGGTCTATGCAATAAAGGTTGTTGCTTCCCTCTAGGCCTTCACCCCTTC CACCCGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020011
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053737, AAH53737
• RefSeq Size: 2691 bp
• RefSeq ORF: 1854 bp
• Locus ID: 56632
• UniProt ID: Q9JIA7
• Cytogenetics: 7 B3
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.