Icos (NM_017480) Mouse Untagged Clone

CAT#: MC204698

Icos (untagged) - Mouse inducible T-cell co-stimulator (Icos), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Icos
• Synonyms: AILIM; CCLP; CRP-1; H4; Ly115
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034852
  GGAGGAGAAGACTGCAACTGCTCCTGGCAGACATGAAGCCGTACTTCTGCCGTGTCTTTGTCTTCTGCTT CCTAATCAGACTTTTAACAGGAGAAATCAATGGCTCGGCCGATCATAGGATGTTTTCATTTCACAATGGA GGTGTACAGATTTCTTGTAAATACCCTGAGACTGTCCAGCAGTTAAAAATGCGATTGTTCAGAGAGAGAG AAGTCCTCTGCGAACTCACCAAGACCAAGGGAAGCGGAAATGCGGTGTCCATCAAGAATCCAATGCTCTG TCTATATCATCTGTCAAACAACAGCGTCTCTTTTTTCCTAAACAACCCAGACAGCTCCCAGGGAAGCTAT TACTTCTGCAGCCTGTCCATTTTTGACCCACCTCCTTTTCAAGAAAGGAACCTTAGTGGAGGATATTTGC ATATTTATGAATCCCAGCTCTGCTGCCAGCTGAAGCTCTGGCTACCCGTAGGGTGTGCAGCTTTCGTTGT GGTACTCCTTTTTGGATGCATACTTATCATCTGGTTTTCAAAAAAGAAATACGGATCCAGTGTGCATGAC CCTAATAGTGAATACATGTTCATGGCGGCAGTCAACACAAACAAAAAGTCTAGACTTGCAGGTGTGACCT CATAAGCTGGAACACCCGGACCCACAGAGGAAACGCATGGACTAGTTCCCCTGAAACTTGAATGGAGAAA TTCTTCTATTTTCTGGACCACAGGGCACCTGACTTGATTTAACTACAGACAACATTCTGCTGGTGTTTTG TTTGTCTGGATCAGTGACTATCAGTCATTCAGAATTTCAGCAGACTGCCCTGGGTTTGCTGAGTCCTTTT AAGATGAACCCCTTCTTAAGGAAGACCCAGCTCATATATATTCAACAAACAGACCTCACTGGGATAGAAT GCCCCTCTCTCCGTGCCTGATTCTAGCTATGCACTGGCCAGGAAAACAAACATATCTCCAGCATTTTTTT ACAAAAAATGCCGAGGGTATGAATCTGTAAAGCACACAGGCAGCCAATGGCCACCATCTGTCCTCATTTT TCAGATTCCATTTTCTTTCCATGGAGATCAGCATTCTTTCTAGAATCAGACAGTAGAGGGAGATGCTTCA CAACAGAAGCGCTTGTGTTTCTGCGATTTTGATGTATATACTTTATCGAACTCATGCATTAGAACCAACA TGCTCTCTTCCAATTCCTAGACTCTAGCTGATCACTGCTTCAGGGCTTAGATGCCTGCTCTTGCCTTCAA ATTTCCCCTTAAAGATATGTCCACATGTCTACTTGGCAGCCTGCTGCCACTCCAAATAGGAAGCTCAGCC TACATTCCCTCCCACCCCTGTTGCTCAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_017480
• Insert Size: 603 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034852, AAH34852
• RefSeq Size: 1378 bp
• RefSeq ORF: 603 bp
• Locus ID: 54167
• UniProt ID: Q9WVS0
• Cytogenetics: 1 30.6 cM
• Gene Summary: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).[UniProtKB/Swiss-Prot Function]