Trpt1 (NM_153597) Mouse Untagged Clone

CAT#: MC204663

Trpt1 (untagged) - Mouse tRNA phosphotransferase 1 (Trpt1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpt1
• Synonyms: AU079016; AW045591; Tpt1; Tpt1h
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027575
  GCAAGTCTCGCCCGCTGGGCCTCAAGTTCCCTTGCTGAACTAGGGTGGGATGAGCAGTGCGGTTCTTATC CTGAGCTTTTGTAACTTTCTGGGCAGAGAACCCACACTCCCTGCTCCCTACAGGTCTTGATCATGAACGC CCCTGGAGGAAGGAGGAAGGAAGGTAGAAGGACTCATAGACCCCGGGAACAGGACCGAAATGTGCAGCTG TCTAAGGCTCTGTCCTATGCCCTTCGCCACGGGGCCTTGAAGCTGGGACTTCCCATGCGAGCTGATGGCT TTGTGCCCCTGCAAGCCCTCCTGCAGCTGCCCCAGTTCCACAGCTTTTCCATTGAAGATGTGCAGCTCGT GGTCAATACCAACGAAAAGCAGCGGTTTACCCTGCAGCCAGGCGAGCCGAGCACTGGGCTCCTCATCAGG GCCAATCAGGGCCACTCCCTACAAGTCCCTGAGTTGGAACTGACGCCCCTGGAGACACCACAAGCCCTGC CCTTGACACTAGTCCATGGTACATTCTGGAAGCACTGGCCATCTATTCTACTAAAGGGCTTGTCACGCCA AGGAAGGACACACATCCACTTGGCCTCAGGATTGCCTGGGGACCCTGGTGTCATCAGCGGCATTCGTCCA AATTGTGAGGTGGCGGTGTTCATCGATGGACCCCTAGCCCTCACAGATGGAATCCCTTTCTTCTGCTCCG CCAATGGTGTGATTCTGACTCCAGGGAATGCTGAAGGCTTCCTGCTTCCCAAGTACTTCAAGGAGGCCCT GCAGCTTCGACCTACACGAAAGCCTCTCTCCTTGGCTGGTGATAAAGAGACAGAGACTCAGAGTGGCCCC AAGCTCAGTTCCAGAGGAGGAAGAAGAAAGATCCAACAATAAAATATTTATTTATTAAAAAACCTGAAAC AGACACAGAAAGAAAGTCCAGCTTCAAACCAGAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_153597
• Insert Size: 618 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027575, AAH27575
• RefSeq Size: 966 bp
• RefSeq ORF: 618 bp
• Locus ID: 107328
• UniProt ID: Q8K3A2
• Cytogenetics: 19 A
• Gene Summary: Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and is protein-coding.