Chmp3 (NM_025783) Mouse Untagged Clone

CAT#: MC204657

Chmp3 (untagged) - Mouse vacuolar protein sorting 24 (yeast) (Vps24), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chmp3
• Synonyms: 25.1; 4921505F14Rik; 9130011K15Rik; CGI-49; D6Ertd286e; NEDF; Vps24
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049964
  CCTGCCTGGGCGCAGGGAACGGAAAGTCGGAAGGGGTAAGAGGAGTTCAGTTCATCATGGGACTCTTTGG AAAAACCCAGGAGAAGCCTCCCAAAGAGCTGGTTAATGAATGGTCACTGAAGATCAGAAAGGAAATGAGA GTTGTTGACAGGCAAATAAGAGATATCCAAAGAGAAGAAGAGAAAGTAAAACGGTCTGTGAAAGATGCAG CCAAGAAGGGCCAAAAGGAAGTCTGTGTGGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAAGCCGTGAG CAAGCTCTATGCCTCCAAAGCACACATGAACTCTGTGCTCATGGGCATGAAGAACCAACTCGCGGTCCTG CGAGTAGCTGGTTCTCTGCAGAAGAGCACAGAAGTGATGAAGGCAATGCAGAGTCTTGTGAAGATCCCAG AAATCCAGGCCACCATGCGGGAGCTGTCCAAAGAGATGATGAAGGCTGGAATCATAGAGGAGATGTTAGA GGATACATTTGAAAGCATGGATGATCAAGAAGAAATGGAAGAAGCAGCTGAGATGGAGATTGACAGAATC CTCTTTGAAATCACAGCAGGAGCCTTGGGCAAAGCACCCAGTAAAGTAACCGATGCCCTTCCTGAGCCTG AACCCGCAGGAGCGATGGCTGCCTCCGAAGAGGGAGAAGAGGAAGAAGATGAAGAGGACCTGGAGGCTAT GCAGTCACGGCTGGCCACACTCAGAAGCTAGGGCCACCCAGTTGGGGGTCTCAACTCTCTGGTGTCCTCC TCTAGGTGTGTATCTGGCCTCTGAGGAGTCACCATTTCTTGTATCTCTTGCACTACACCTCTGCTGTGAA GCATTACATTTGGAGAGGGTTCTGTGCTGGTATATCTTCACGATCTGCAGAGGATTTAAGGATTGCTTTT ATGAAGGTGGTGTAATCAAATGTATTATTTTCAGGAGCATAAATAGCAGAAGTATCTTCCTTGGGGGAGG GCAAAGAAATCCATTTTCTCATGAAGCAATTTTGAGAAGAGTAGATTGAATGATGAGGACTCTATATTTG GTGTGTATGTGTAAAATAATATATTAATGGATGTCAATAAACTTCTGCTTTAGCTCTTGGTCTCATTGTA AATGTCACATGCAATGAACTCAAGATATTAATCTCCCAGATTGTCTCAGTGTTGGCCTTGGAAAGAGGAC ATGCTATCTCCAAGCATCGGGACATGGGAGCTTAGCAGAAGTACCTTCAGCTCCGGGGCCGCTGGTCCTG CTCTTCACAGGCTGGTTGTGGGGGCCACTCTTGTGCATTTTGTCATTTGTCTAACCTTCGGGTTAACGTG CTGTGTTGACTGAATGACCATATCATACACTGGTGTGTACAAGGGAGTTTTTACAGCTGGTAGGCATTCT TCCCCACTGCAAGTGAAATGCAGGACAGTTAGATCTGGGATCAGGACTCTTGCAGTCCATCCCTGCTTGG AGCTAAGTGTCTTGTACCCAGGGACGTAGCCTTAACCATGAGCAGGAAGAGCTAACCAGGAAGAGGTTTG GATTATGCAGTGAAACACACACAGCAGAGCATGCTTTAGATGCATTGAGAAATAGCCCAGCAGTAGCACT GTGTGGAGCAGTTTGAAAAGCTTTCTGGTATGCATGATCTTCACAAATGAGGACAGCTGTCATCTGAAGT CACAAACCCTAAAGTACAATAGCCAGTATCCGCCAGTATTGTACAGAATGGCTTTGTTACCCAAAACACA ACCCATGTGCTGTTCATTGCTTCCTCTAATCCCTTGGCACCCACTGACCCGGTTGCTGTCTCCATGGGCT TTCCTTTCCCAGCCTTTCAGTATGTCTTCCCTCACGTGGTGATATGCATTTACGTTCCTCCGTGGATTCA TGACTTGACTGTTCAGCCCTTCTCATTGAGCAGCGTTCCATTTTCTAGCTGTACCACAGCTTGTCTTGAA GGCCACCTGGACTGCTTCTGAGTTTGGGAGTTTGAAGCAATGTCTGTAGCAGGTTTCCCTGTGTGTTTTA ATTCCTCTGGATACCAGAGAGCTCAACCCTGGGATCATGTGGTCAGCACAAACCTGTGCTGTAAGGTAAA CCAGCCGTGCAGAGCACGTACTGTCCCCGTCTCAGGAGGAAGAGTTCTGCCTGCCATCCTGTGGCTTTGG CAGAATTTGTCATGAGGGATTTGTGTTGAAGTACCCTGGGCATGTTTTTATACTTGCCTTCTCTGTATCT TGTTTGAAAATGTTGACATAGTGGCCTTGAACTCATGACCTGTCTGCTCTACCACCACACTGAGCTTGCC GCCTGGATTTCTTCGTTGTTGGTGTTTGTTCATTTTATAACCTGGATTGTTTGTTTTCTGATTATTCAAG TTTAAGGACCTCTTACAGATTTTGAACCAATCATCTATCAGATTATATCCTATTCTCTGTTCTATTCTCT CATTGTTGACTGTTTTTCAAAGCTGAAGGTTTCTTTTTTACTTTTAATAAAACCCTGCTTCTCAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025783
• Insert Size: 675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049964, AAH49964
• RefSeq Size: 2573 bp
• RefSeq ORF: 675 bp
• Locus ID: 66700
• UniProt ID: Q9CQ10
• Cytogenetics: 6 32.17 cM
• Gene Summary: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor (By similarity).[UniProtKB/Swiss-Prot Function]