Cdt1 (NM_026014) Mouse Untagged Clone

CAT#: MC204648

Cdt1 (untagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdt1
• Synonyms: 2610318F11Rik; AW545653; C76791; Ris2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048076
  GAAAGTTGCGCGGAATCCGCGGGAACTACCTTTTTGGCGCCATGGCGCAAAGTCGTGTTACCGATTTCTA TGCGTGCCGTCGCCCTGGCCTTACGACTCCGCGGGCCAAGTCGATCTGTCTCACCCCGAGCCCTGGTGGC CTCGTGGCTCCTGCGTTCACCCGGAGCAGCAGCCGCAAGCGCGCCCGGCCCCCAGCCGAACCCGGGAGTG ACCAGCCCGCGCCGCTCGCGCGCCGGAGGTTACGGCTGCCTGGATTGGACTCCTGCCCCAGTTCTCTGCC TGAGCCCAGTTCCCCAGCTGAGCCCAGCCCTCCAGCTGACCCTAGCCCTCCAGCTGACCCTGGCTCCCCC GTTTGCCCATCCCCCGTCAAGCGAACAAAGAGTACAACTGTTTATGTTGGTCAACAGCCGGGCAAGATCC CCTCAGAGGACTCCGTCTCTGAGCTCCAGTCCTGCCTGAGGCGGGCACGGAAGTTGGGAGCCCAGGCACG GGCCCTGAGAGCCCGAGTCCAAGAGAATGCTGTGGAGCCTAGTACCCCAGATGCCAAGGTGCCCACAGAG CAACCATGTGTCGAGAAAGCTCCTGCCTACCAGCGCTTCCATGCTCTGGCTCAGCCTGGTCTCCCAGGCC TTGTCCTACCCTACAAGTATCAGGTGCTAGTTGAGATGTTCCGCAGCATGGACACCATTGTGAGCATGCT CCACAATCGCTCTGAGACTGTGACCTTTGCCAAAGTCAAGCAAGGTGTTCAGGAGATGATGCGCAAGCGC TTTGAAGAGCGCAATGTGGGCCAGATCAAAACCGTGTATCCCACGTCGTATCGCTTCCGCCAGGAGTGCA ATGTCCCCACCTTCAAGGACAGCATCAAGAGATCTGATTACCAGCTCACCATCGAGCCCTTGCTGGGCCA GGAGGCTGGCGGTGCCACCCAGCTCACAGCCACGTGCCTCCTGCAGCGCCGGCAAGTCTTCCGGCAGAAC CTGGTGGAACGTGTCAAGGAACAGCACAAGGTCTTCCTGGCTTCACTGAACCCCCCCATGGCGGTGCCGG ACGACCAGCTGACCCGCTGGCATCCGCGCTTCAATGTGGACGAGGTGCCTGACATTGAGCCAGCTGAACT GCCCCAGCCTCCTGTCACAGAGAAGCTCACCACTGCCCAGGAAGTGCTGGCCCGTGCCCGGAGCTTGATG ACACCCAAGATGGAGAAGGCCCTGAGCAACCTGGCCCTGCGCTCGGCTGAGCCCGGTAGCCCTGGGACCT CTACTCCACCACTCCCGGCCACTCCGCCAGCCACCCCACCTGCTGCCTCTCCGAGCGCCCTGAAGGGTGT GTCCCAAGCACTGCTAGAGCGGATAAGGGCCAAGGAGGTCCAGAAGCAGCTGGCAAGGATGACACGGTGC CCCGAGCAGGAGCTTCGCCTGCAGCGGTTAGAGCGTCTGCCAGAGCTGGCCCGCGTGCTGCGCAATGTCT TCGTGTCTGAGCGGAAGCCGGCACTCACTATGGAGGTGGTCTGTGCAAGGATGGTGGACAGTTGCCAAAC TGCTCTGAGTCCAGGGGAGATGGAGAAACATCTGGTGCTCCTGGCAGAGTTGCTGCCGGACTGGCTCAGC CTGCATCGCATCCGCACGGATACCTACGTCAAGCTGGACAAGGCTGTTGACCTGGCTGGCCTCACTGCGA GGCTGGCCCACCACGTCCACGCCGAGGGGCTGTGACTTTGAGCTCCTTGCCTGTTTCTTTCATCAGTACA CGACGCACTTACGCCTTTAAGCCTCGCCAGTGTGGGCAGCTATCGTTGCCCATGGATCTCATAAAGTGCT GGCATTAAGTTGCTTCCTGAGGCTTTGGGGCATCCCAGACTCAGCTCTAGGGGAAGTAGACTCTGAAGAG TAGGGTATGTTGGATGGCCATTGCACAAACTACTCAAGCATTAGGTATGGTCAAGACCCAAGATCAAGGT CCAGGTGGAAGCCTAGGCTGGCATAGCCTTGCTTCCCCATGAGACTTAAGAATCACACAGACCTTGGACT TTCCTGATTTCACGGGACGCTGCTCTGAGAGTGAAATTGGGCCTTCTGTAAATATGTGAAGTGTGGTTTC TTTTCAAACCTTATATGGCCCTGCATGTGACTGCTAGTTTTGGCTTTTAATAAAGTCATGTAAGATTTAA ATAAAATACTACTGAGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026014
• Insert Size: 1674 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048076, AAH48076
• RefSeq Size: 2245 bp
• RefSeq ORF: 1674 bp
• Locus ID: 67177
• UniProt ID: Q8R4E9
• Cytogenetics: 8 E1
• Gene Summary: Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments (By similarity). Potential oncogene (PubMed:11850834).[UniProtKB/Swiss-Prot Function]