Etfbkmt (NM_177101) Mouse Untagged Clone

CAT#: MC204647

Mettl20 (untagged) - Mouse RIKEN cDNA 4833442J19 gene (4833442J19Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Etfbkmt
• Synonyms: 4833442J19Rik; AI256744; ETFB-KMT; Mettl20
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048711
  CGAAAAAGGAGACCTACCGTACTTGTTTTTTTTTTTCTCCTGCACGTGAAGGGCGAGTTCCACTTGCTGA TCGAATAAAGTCAGAGATCCAGAAATTCCAGTGTCAATTAAGTGGAAAGATGGACCCTCATTCTATCTGC TTGGGAAACAGTCCTTATTAATAGCAACACTGACAGAACCTGAGGTTTGGAGATGTACTAATGGCTTTCA GCCTGTGTTGGAAAGCTCCCAGGAGCCCATGGAGTTTCCTGCAGGCTGTGAACAATGGGTCTCCCCTGTT TCTCTGGAGGACAGTGGGGAGCTGTTTGGATCCTAAGATGAAAGCCTACCTGGAAGAGAACACTGAAGTC ACCAGCAGTGGTAGCCTTACCCCGGAAATCCAGTTACGGCTTTTAACCCCCAGGTGCAAGTTTTGGTGGG AAAGGGCTGACCTGTGGCCCTACAGTGATCCCTACTGGGCTATTTACTGGCCAGGAGGCCAGGCTCTGTC TAGGTATCTTTTGGATAACCCTGCTGTTGTTAGAGGAAAGTCTGTCTTAGATCTTGGGAGTGGATGTGGA GCTACAGCCATTGCTGCCAAGATGAGTGGAGCATCAAAGATCTTGGCCAACGACATAGACCCCATTGCAG GAATGGCGATTACACTAAATTGCAAATTAAATGGACTGAATCCTTTTCCTGTTTTAACTAAAAACATTTT GAATACCCAACAAGGTAAGTTTGATCTCATTGTTCTTGGAGATATGTTTTATGATGAAGACCTTGCAGAC AGTCTTCATCTGTGGCTGCAGAACTACTTTTGGACCCATGGAACCCGAGTACTGATTGGTGACCCTGGGA GGCCCCAGTTCAGTGGACATAGCATTCGGCATCAACTGTACCAACTAGTAGAATACACACTTCCAGAGCC AACTCAGCAAGAGAACAATGGACTGACCACAAGTGCTGTATGGGATTTTCATCCCTGATTTGTTAAAGTG CTTTCAAGTATGAATATAGTTCTGTTTGTGTTAAGTTCTAAAAATATCCTCTGTAGGAGGTATTCAACAG TTTAAAGAATGCAATTCAGTGGCTTGTGGTGGATTGGGCCTTGAATACTACCACTTGGGAGGCTGAAGTA GGCGCATCTCTGTGACTTTGAGGACAGCTTGGTCTATAGTGAGTTCAAGGACAGCCAGAGCTATATAGTA AGACCCTGTTCCAAAAATAGATAGATAGATAATAAAGTAAATTTCTTCATTCCTTTGCTGTGACACCTTG AATAACTGTATGGTTTATTAAAGTTAGTTATGGTTATGTTCCCATTTCACATACCAAGGTGTCAGGATTT TATAACAAAGCACTCTATCATATACAATTGCATATGTAGAAGGTATATTTATATATGTATGTATGTATGG ATGTAATATATATAATTTGCTTCAGTATTAATAAAATATCTCTGGAAAGATAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_177101
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048711, AAH48711
• RefSeq Size: 1513 bp
• RefSeq ORF: 768 bp
• Locus ID: 320204
• UniProt ID: Q80ZM3
• Cytogenetics: 6 G3
• Gene Summary: Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria. Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1, and encodes the longer isoform (a). Variants 1-4 all encode isoform a.