Enpp2 (NM_015744) Mouse Untagged Clone

CAT#: MC204624

Enpp2 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Enpp2
• Synonyms: AT; ATX; Auto; E-NPP 2; lysoPLD; N; Npps2; Pd; PD-; PD-Ialpha; Pdnp2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058759
  GAAATCGAGCTCTGCACGAATCAGATGCGCCTGTCAACTTCCCAGGTGGGATTGCTTGGAGTTAACAGCC TGAACGCAGAGCCCCGAAAGCAGAGCATTCAGGGCAAGCAGAGAACACCCTGCAGAGGTTTTCCAAGAAT CCCTCGGCATGGCAAGACAAGGCTGTTTCGGGTCATACCAGGTAATATCCTTGTTCACTTTTGCCATCGG CGTCAATCTCTGCTTAGGATTCACAGCAAGTCGAATTAAGAGGGCCGAATGGGATGAAGGACCTCCCACA GTGTTATCTGACTCTCCATGGACCAACACATCTGGATCCTGCAAAGGTAGATGCTTTGAGCTTCAAGAGG TTGGACCTCCTGACTGTCGGTGTGACAACCTATGTAAGAGCTACAGCAGCTGCTGCCATGATTTTGATGA GCTCTGTTTGAAAACAGCTCGAGGCTGGGAGTGCACCAAAGACAGATGTGGGGAAGTACGAAATGAGGAA AATGCCTGTCACTGCTCAGAAGACTGCTTGTCCCGGGGAGACTGCTGTACCAACTACCAAGTGGTCTGCA AAGGAGAATCACACTGGGTAGATGATGACTGTGAAGAAATAAGAGTCCCCGAATGCCCTGCAGGGTTTGT CCGCCCTCCGTTAATCATCTTCTCTGTGGATGGATTCCGTGCATCGTACATGAAGAAAGGCAGCAAGGTT ATGCCCAACATTGAGAAACTGCGGTCCTGTGGCACCCATGCTCCCTACATGAGGCCTGTGTACCCTACAA AAACCTTCCCTAATCTGTATACGCTGGCCACTGGTTTATATCCAGAATCCCATGGAATCGTTGGCAATTC AATGTATGACCCTGTCTTTGATGCTACTTTCCATCTTCGAGGGCGAGAGAAGTTTAACCATAGATGGTGG GGAGGCCAACCGCTATGGATTACAGCCACCAAGCAAGGGGTGAGAGCCGGGACATTCTTTTGGTCTGTGA GCATCCCTCACGAGCGGAGAATCCTAACTATCCTTCAGTGGCTTTCCCTGCCAGACAATGAGAGGCCTTC AGTTTATGCCTTCTACTCCGAGCAGCCTGATTTTTCTGGACACAAGTACGGCCCTTTTGGCCCTGAGATG ACAAATCCTCTGAGGGAGATTGACAAGACCGTGGGGCAGTTAATGGACGGACTGAAACAACTCAAGCTGC ACCGTTGTGTGAATGTTATCTTTGTTGGAGACCATGGAATGGAAGACGTGACATGTGACAGAACTGAGTT CTTGAGCAACTATCTGACTAACGTGGATGATATTACTTTAGTACCTGGAACTCTAGGAAGAATTCGACCC AAGATTCCCAATAATCTTAAATATGACCCTAAAGCCATTATTGCTAACCTCACGTGTAAAAAACCAGATC AGCACTTTAAGCCTTACATGAAACAGCACCTTCCCAAACGTTTGCACTATGCCAACAATCGGAGAATCGA GGATCTCCATTTATTGGTGGAACGCAGATGGCATGTTGCAAGGAAACCTTTGGACGTTTATAAGAAGCCG TCAGGAAAATGTTTTTTCCAGGGTGACCACGGCTTTGATAACAAGGTCAATAGCATGCAGACTGTTTTTG TAGGTTATGGCCCAACTTTTAAGTACAGGACTAAAGTGCCTCCATTTGAAAACATTGAACTTTATAATGT TATGTGCGATCTCCTAGGCTTGAAGCCAGCTCCCAATAATGGAACACATGGAAGTTTGAATCACCTGCTA CGCACAAATACCTTTAGGCCAACCCTACCAGAGGAAGTCAGCAGACCCAATTACCCAGGGATTATGTACC TTCAGTCTGATTTTGACCTGGGCTGCACCTGTGATGATAAGGTAGAGCCAAAGAACAAATTGGAAGAACT AAATAAACGCCTTCATACCAAAGGATCTACAGAAGAGAGACATCTCCTGTATGGACGACCTGCAGTGCTT TATCGGACTAGCTATGATATCTTATACCATACGGACTTTGAAAGTGGTTACAGTGAAATATTCTTAATGC CTCTCTGGACTTCTTATACCATTTCTAAGCAGGCTGAGGTCTCTAGCATCCCAGAGCACCTGACCAACTG TGTTCGCCCTGATGTCCGTGTATCTCCTGGATTCAGTCAGAACTGTTTAGCCTATAAAAATGATAAACAG ATGTCCTATGGATTCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTG TAACCAACATGGTTCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAA GAAATATGCGTCAGAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACAATGGC TTACGTGACATTGAGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACT ACAGCATCATCACCAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTC TTCTTTCATCCTTCCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGG GTAGAGGAACTCATGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTTTGGATTTCT ACCGGAAGACTAGCCGTAGCTATTCGGAAATTCTGACCCTCAAGACATACCTGCATACATATGAGAGCGA GATTTAACTTCCTGGGCCTGGGCAGTGTAGTCTTAGCAACTGGTGTATATTTTTATATGGTGTTTGTATT TATTAATTTGAAACCAGGACATAAACAAACAAAGAAACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_015744
• Insert Size: 2589 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058759, AAH58759
• RefSeq Size: 2853 bp
• RefSeq ORF: 2589 bp
• Locus ID: 18606
• UniProt ID: Q9R1E6
• Cytogenetics: 15 D1
• Gene Summary: This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2, also known as beta) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.