Nek4 (NM_011849) Mouse Untagged Clone

CAT#: MC204605

Nek4 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nek4
• Synonyms: Stk2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057939
  GGTGGGCCGGGCCTCGAAGCTCCTGCCCGCGGTGGGCCGCGTTTATGGAGTACTTCACCTCCCGTCTGGC ATGAGCGGGGCCTCCCGCAGCCTTACGCGCCGCTGCCACAGCTTCCCGACTGTAGGCCTCCTTCGGGAAC ATGCCCCAGGCCGCATACTGCTACATGCGGGTCGTGGGCAGGGGTAGTTACGGGGAGGTGACGCTCGTGA AGCATCGGCGAGACGGCAAGCAGTATGTCATCAAAAAGCTGAACCTCCGAAATGCCTCCAGCCGAGAGCG TCGAGCTGCTGAGCAGGAAGCTCAGCTCCTATCTCAGTTGAAGCACCCCAACATTGTCACCTACAAAGAA TCATGGGAGGGAGGAGACGGTCTGCTCTACATTGTCATGGGCTTCTGCGAAGGAGGTGATCTGTACCGGA AGCTCAAGGAGCAGAAAGGGCAGCTTCTGCCTGAGAGTCAGGTGGTGGAGTGGTTTGTCCAGATCGCCAT GGCCTTGCAGTATTTACATGAGAAACACATCCTTCATCGAGATCTGAAAACTCAGAATGTCTTCTTAACA AGAACAAACATCATCAAAGTGGGGGACCTAGGAATTGCCCGCGTCTTAGAGAATCACGGTGACATGGCGA GCACCCTCATTGGCACACCCTATTACATGAGCCCTGAGCTGTTCTCCAACAAACCCTACAACTATAAGTC GGATGTTTGGGCTTTGGGATGCTGTGTATATGAAATGGCCACCCTGAAGCACGCTTTTAATGCAAAAGAC ATGAATTCTTTAGTTTATCGGATTATTGAAGGAAAGTTGCCGCCAATGCCAAAAGTTTACAGCACAGAGC TGGCTGAGCTGATAAGAACAATGCTGAGCAGAAGGCCTGAGGAGAGGCCCTCTGTGCGGAGCATCCTGAG GCAGCCCTACATAAAACACCACATCTCCTTGTTCTTGGAGGCCACCAAGGCAAAAACTTCCAAAAATAAT GTTAAAAACTGTGACTCCAGAGCCAAGCCTGTTGCTGCTGTAGTTTCCAGAAAGGAAGAATCAAATACTG ACGTGATACACTACCAGCCACGCTCTTCTGAGGGCTCTGCTTTGCATGTCATGGGTGAAGATAAATGTTT GTCCCAAGAGAAACCAGTGGACATTGGTCCCTTGAGATCACCGGCTAGTCTTGAAGGCCACACTGGCAAA CAAGACATGAACAATACTGGGGAGTCATGTGCCACAATCAGTAGGATAAATATCGATATCTTACCTGCAG AAAGGAGGGACTCAGCGAACGCTGGTGTAGTTCAGGAGAGCCAACCACAACACGTGGATGCCGCTGATGA AGTAGACAGTCAGTGCAGTATTTCTCAAGAGAAGGAGAGGCTACAGGGCAATACCAAGTCCAGTGATCAG CCTGGAAATCTCCTTCCCAGACGGTCCTCTGATGGTGGTGATGGGGAAGGGAGTGAACTGGTGAAGCCAT TGTATCCCTCAAATAAAGACCAAAAGCCAGACCAGGATCAAGTTACTGGTATTATAGAAAATCAGGACAG CATCCACCCAAGATCCCAGCCACACAGCTCTATGTCTGAACCTTCTTTGTCCCGGCAGCGAAGGCAGAAA AAAAGGGAACAGACTGCCCATAGTCGTACAAAGAGCCAGTTCCAAGAATTACCCCCTCGGCTTTTGCCTT CTTATCCTGGTATTGGGAAAGTGGATATCATAGCAACACAGCAAAATGATGGAAACCAAGGTGGACCTGT GGCTGGGTGTGTAAACAGTTCAAGGACCAGCTCAACAGCATCAGCAAAGGATCGACCATTATCAGCCAGA GAGAGGAGACGGCTAAAGCAGTCACAGGAGGAGATGCTGCCCTCAGGCCCTGCAGTTCAGAGAACTCCAA GTGCAGTTGAGCCACTGAAACCACAGGAAGAAGACCAACCCATCCCTGCCCAACGATTCTCTTCTGACTG CAGCATCACTCAGATGAATCACACACTCCCGAGGGAAAAGGAGAAGAGGCTGATGCATGGCCTGTCTGAG GATGAGTTAAGCTCTTCTACAAGCTCAACTGATAAGTCAGATGGGGATTCCAGGGAGGGGAAGAGTCATA CAAATGAAATGAAAGACTTGGTACAGCTGATGACTCAGACTCTGAGGCTGGAAGCTAAGGAGAGCTGTGA AGATCTCCAGGTCCTAAACCCAGGGTCAGAATTCAGACTTCATCGAAAGTATCGAGACACGCTGGTGCTT CATGGCAAGGTTGCAGAAGAGGTGGAACCCCACTGTACGGAGCTGCCTACGGGTATCATACCAGGTTCTG AAAAGATCAGGAGAATCGTTGAAGTCTTAAGAGCTGATGTAATCCAGGGCCTGGGGATTCAACTCTTAGA GCAGGTGTTTGATCTTTTGGGAGAGGAAGATGAGTTGGAGAGAGAGGCACGATTACAGGAGCACATGGGA GACAAGTATACAACTTACTGTGTGAAAGCTCGCCAATTGAAATTTTTTGAAGAAAATGTGAGTTTTTGAG ATTTGTTCTAACATGCTGCCAGAACTAAAGATCTATTTTCAAAAGTTTTTCATCTTAAAAAAAACACCAC CACCACCATCACCAACCCTGTTCCAGTCATTCAGAGGCTGGTCACCGCCATCCTTTGCTTGATATCTCCT TGGAGTCCTCTTACTATAAAGAACTGGAGAGCAGACTAATGCGAAACAAGTTCACAAAAGAAAAAAAAAC TTTGAATATCATATGTTTATGAGGAAAAATGTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011849
• Insert Size: 2379 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057939, AAH57939
• RefSeq Size: 2778 bp
• RefSeq ORF: 2379 bp
• Locus ID: 23955
• UniProt ID: Q9Z1J2
• Cytogenetics: 14 B
• Gene Summary: Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage (By similarity). Protein kinase that seems to act exclusively upon threonine residues.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).