Rngtt (NM_011884) Mouse Untagged Clone

CAT#: MC204464

Rngtt (untagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rngtt
• Synonyms: AU020997; HCE; MCE1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC043657
  CGAACCTGAATGAAAGTGGCGCGCCGCTGAGGACCCGGGTAGAGCTGCTGCCGTTGGGGTCCGGGCTGGT TTGCTGTGGAGCTATCGGCGAGGCGGCACAGACATGGCTTACAACAAGATCCCGCCGCGGTGGCTGAACT GTCCGCGGCGCGGTCAGCCGGTGGCAGGAAGATTCTTACCTCTGAAGACGATGCTGGGGCCAAGATATGA TAGTCAAGTTGCTGAAGAAAACCGGTTCCATCCCAGTATGCTCTCAAATTATCTAAAGAGCCTGAAGGTT AAAATGAGCTTGTTGGTGGATCTGACAAATACTTCAAGGTTCTATGACAGAAATGACATAGAAAAAGAAG GAATCAAGTATATCAAACTTCAGTGTAAAGGACATGGCGAATGCCCCACCACTGAGAATACTGAGACATT CATTCGCCTGTGTGAGAGGTTTAATGAAAGAAGCCCACCTGAACTTATAGGTGTTCACTGCACCCATGGC TTCAACCGCACTGGTTTCCTTATATGTGCCTTTTTGGTGGAAAAAATGGATTGGAGTATTGAAGCAGCAG TTGCTACATTTGCCCAAGCCAGACCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCG CTATGGAGATATAGAAGAAGCACCACCACCACCTGTATTGCCAGATTGGTGTTTTGAAGATGAGGATGAA GAAGATGAGGATGAAGATGGAAAAAAGGACTCAGAACCAGGGTCAAGTGCATCCTTTAGTAAAAGGAGAA AAGAGCGGTTAAAACTGGGAGCTATTTTCTTGGAAGGCATAACTGTTAAAGGTGTAACTCAAGTAACAAC TCAACCCAAGTTAGGAGAGGTGCAGCAGAAATGTCATCAGTTCTGTGGCTGGGAAGGGTCTGGGTTCCCT GGAGCACAGCCTGTCTCCATGGACAAGCAAAATATTAGACTTTTAGAGCAGAAGCCTTATAAAGTAAGCT GGAAAGCAGACGGCACTCGTTACATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGACAGAGA TAATTCCGTTTTTCATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGCATGCATTTATCAAAT ACTCTTTTGGATGGGGAAATGATCATTGACAAAGTAAATGGACAGGCCGTCCCAAGATATTTGATCTATG ACATAATTAAGTTCAATGCACAACCAGTTGGAGACTGTGATTTTAATATTCGTCTACAGTGTATTGAACG TGAAATTATAAGTCCACGACATGAAAAAATGAAGACTGGACTCATTGACAAAACACAGGAACCATTTAGT GTCAGACCTAAACAATTTTTTGACATCAATATTTCAAGAAAGCTTCTGGAAGGAAATTTTGCCAAAGAAG TCAGCCATGAAATGGATGGACTTATTTTTCAGCCTATTGGAAAATACAAGCCTGGTCGATGTGATGACAT TTTGAAATGGAAACCTCCCAGTCTAAACTCTGTGGATTTTCGACTTAAGATAACAAGAATGGGAGGAGAA GGGTTGCTTCCACAGAATGTTGGCCTTCTCTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGG TGACAAAAGAACTAAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAACAATAGCTGGGTCTT CATGAGACAGAGGATAGACAAAAGTTTCCCAAATGCCTACAACACAGCCATGGCTGTGTGCAATAGCATC TCGAACCCTGTCACCAAGGAGATGCTGTTTGAATTCATTGACAGATGTGCAGCAGCCGCCCAGGGACAGA AGCGGAAGTATCCCCTGGACCCTGACACGGAGCTCATGCCGCCCCCACCGCCCAAAAGACTGCATCGGCC AACCTAGTCCGTGCCTCTGAGAGGAAGAAAAATGCTGTTGTCTCATTTTCTCAACCAGAGAAATTAAAGA GACGAGCGGCTTCGTACTGGTACACGTGTTTTAAGGGAAGACATAGTCAGCCTGTATATATTTGGTACAT TTGTTTCCTTGGAACTACAGTACATCATGGACCTAAATATTTTATTTATACCTGATAAATTCAGCCTTAC CCTGTGGAGTCTACAGATTGAAATACCCAAAGGTTTAAATCAGATTGAAGTTAATGAAATAGAGGCCTTT TGTATGTGAATAGATACCTTTACATTTGATTCATAACTAGCAATCTGAAGCTGTAAGCATATTGAGTTTT TTAAATGTTGGATTTTAAGTTTACCTCTTTAAAATGTGGGTACTGGCATTAGAAATTTTCTCACTAACTT ATCATGGATTTCTTATATTTCTCAATTTTTTATATTCTGAGCAATGACAAGGTCAAGTATTATGTACATT AAACAGTTTGGTCTGGCTATACCATTTTAAGAAGTTCTTCTGGTTTTGTTTTCTGTGAGAGTGGGGTGTA AAAACAGTAATGAAGCTCCATTTTCTTGTGAATTGTATCCTTTCTTTAGAAACTATTTTGAGCATTTTAC TATGTCTCGTGTTTGAACCATGACGTCAGATGTCTGGGGTTCCCATCCTGGCAGTGGTTTTGAGTTCCTT TGTGGCACTTACCTTCATGTGCTGCTGTCTGGGAGAGTTGCTGCTTTCAGACAGCAGGGCTCCTCCTGCT TTACCCACTCACTGTGATCCCTGGGAACAGCAGCAAGCACAACCGCGTTGTGTTATTTCCCTCAAACAGT GCTTCAGACACAGTGGCAGCTTTGCCTAGAAGGTCATGTCAGCTGAACAAAGTGTTGTCTAGGAGATGCT GGCCTCCCATGGACAGATGCAGAGGCGTTGCTGAGACTGGTCATGGAGGACGTGAAGATGTTGCTCCCAC TGCTCCTGCTGCACCTGCGCTTGGAGGTGGTTTCCGATCCTGAGCTGCACCTCAGCGTTGACTCTGGACA GTAGGTCCCTGCCGTTGTAAAACTTGTTCTGATTGGCTTGTGAAGGAATTCAGAATAGCAACTAGCAAGC AGCTTACTTAATGTATTTATTTTAGAGACACAGGTTTTAAGTATATGTATTTAAAACAAATGTTCACGAT CTGTATTTTATATATGTATAAGCATATCTGAAAGTATATGCAACAGTGTATATTCTTATATTGATACAGC CAGAAATGAAAATCCAAATGCTTTACCTAGCAGGCTAAGGATCTGATGGAATTTTTATTTGGCAAAGTAC AGATTAACCAAAACTGTATTTATATGAACCTGAGTTTTAATGTTTTTAAACGAACTTTACATCTGCAGTG CTTTTCTGTATGGAATACTAGGGCTGTGCCTAGACTTATGCCGCTATTGTTCCCAAGTTATGTAATATGT TAGCAAGTTTATTCAAAATGCCATAATAATTTTGTCTTAGGTTGCTTTTGTACATTTGGGCACTGAAGGA ATTTAAACACCTGACTGTTACAGGATTGTTGAGGGGAGATTGTCAAGGTTCCTTCTGTCGTTTACATGGT GGATCCAGAACTGCCTGTGGACTCCAGGCAGCCTAGTGCCTGCTCCCCAGGGTCACTGAATCTTTTCTCC ATAGATGGTCTGCTCCCAAGCCGGTGCAAAAGCGGGGTCCTTTTGAAGATACAGTAACGTGCTTTGTCAG TGATCTCTGATAGAATTAAAATGTTATGGTAACATTAACCTTTCAAATGAAATGGAAAGAGCATTATATA GGACTTACTTTTTCAGCTTATCCTTTCCTTTCAATTGTGGTGACGTAATCATTGGCCTTAAAAGCAGACC CCATGTAGTCCCCAACCGCATCACCTCCTGGAGTATCCTTACAGCCATTTAGGCTGTTGGACGAAAGGTA GGATTTGGACATCTTGAAGGAAAACTTCCCTTCTCGGCTTATGTTACAACAGCTGTTTTCTTTAGCTGAT ATTTTCCAGACAGGACTAAGAAGACTGTTTCCCTGAGTTATCGCATGGGAAGGCTTGCTTTTTGTTTGGG GAAAAAAAAATATGGATGACTACTGCCTAGATGGTTCCTTTGCTTTCCATGAAATGCCAGGACATTCCTT CTCTGATTGTGTCAGTGTGTGTTGTAATAAAGCTACTGATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011884
• Insert Size: 1794 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043657, AAH43657
• RefSeq Size: 4152 bp
• RefSeq ORF: 1794 bp
• Locus ID: 24018
• UniProt ID: O55236
• Cytogenetics: 4 A5
• Gene Summary: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoforom (1).