Rgs14 (NM_016758) Mouse Untagged Clone

CAT#: MC204443

Rgs14 (untagged) - Mouse regulator of G-protein signaling 14 (Rgs14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgs14
• Synonyms: RPIP1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030321
  CCACGCGTCCGGTAAGGGCCCGGAGGAGCAGAGCTCTCTCTCTCCACTGCCTGCTTGCCACCCCAAGAGA GTGCTTCCGCGATCCTTGTGGTGGGTGGCCCCCCGTGGAGGGGACCTGTGATCAACGGCGGTATGCCAGG GAAGCCCAAGCACTTGGGTGTCCCCAACGGGCGCATGGTTCTGGCTGTCTCAGATGGAGAACTGACCAGC ACTGCAGGTTCCCAGGCCCAGGGTGAGGGCAGAGGCAGCTCTCTCAGCATCCACAGCCTCCCTAGTGGCC CCAGCAGCCCCTTCTCCACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCAGTCCTTTGAGCGGCTGCTGCA AGACCCACGGGGTCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAATTCAGCGCAGAGAACGTAACTTTC TGGAAAGCCTGCGAACGTTTCCAGCAGATCCCAGCCAGCGACACCAAGCAGCTAGCTCAGGAGGCCCACA ACATCTACCACGAGTTCCTATCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGACAGGCCTGGCTTAG TGAGGAGGTGCTGGCCCAGCCCCGGCCAGATATGTTCCGAGCACAGCAGCTTCAGATCTTCAATTTGATG AAGTTCGACAGCTATGCGCGCTTCGTCAAATCCCCGCTGTACCAAGAGTGCCTGCTGGCGGAGGCCGAGG GACGCCCCCTGCGGGAACCTGGCTCCTCACACCTCGGGAGCCCGGACACAGCGAGGAAGAAGCCAAAGCT GAAGCCTGGGAAGTCACTGCCGCTGGGCGTGGAAGAGTTGGGGCAGCTGCCACTCGCTGAGGGCCCTTGT GGCCGCCCTCTCCGCAAGTCCTTTCGTAGAGAGATGACAGGTGGAGCCATGAATTCGGCCCTGCGACGAG AGTCTCAAGGGTCCCTGAATTCTTCTGCCAGTCTGGACCTGGGTTTCCTTGCCTTTGTGAGCAGCAAATC TGAGAGTCACCGGAAGAGCCTTGGAAGTGGAGAGAGTGAGAGCGAGAGTCGGCCGGGGAAGTATTGCTGC GTGTATCTACCTGACGGCACGGCTTCCTTGGCCCTGGCTCGACCTGGCCTCACCATCCGAGACATGCTGG CAGGCATCTGTGAGAAGAGAGGCCTCTCTCTGCCTGACATTAAGGTCTACCTGGTGGGCAATGAACAGAA GGCCCTGGTCCTGGATCAGGATTGCACCGTGCTGGCAGACCAGGAAGTGCGACTGGAAAACAGGATCACC TTCCAGCTGGAGTTGGTCGGCCTGGAGCGAGTGGTCCGGATCTCAGCTAAGCCCACCAAGCGTCTGCAAG AGGCGCTGCAGCCCATCCTGGCTAAGCATGGCCTGAGCCTGGACCAGGTGGTACTGCACAGGCCAGGAGA GAAGCAGCCCATGGATTTGGAGAATCCAGTGAGCTCAGTGGCCTCACAGACACTGGTTTTGGACACTCCT CCGGATGCAAAGATGAGTGAAGCCAGAAGCATATCCCCCTGCCGCAGTCAGGGATGCCTCCCAAGAACCC AGACCAAGGACAGTCACCTTCCCCCATCGTCCTCCAGTTTGCTGGTAGAAGATGCCAGTAGTTCTACTGG GAACCGGCAGACCTGTGACATTGAAGGCCTGGTGGAGCTGCTGAATCGGGTGCAGAGCAGCGGGGCCCAC GATCAGAGAGGACTTCTTCGCAAAGAGGACCTGGTCCTTCCAGAATTTCTGCAGCTTCCTTCCCAAAGAC CAGGCTCTCGGGAGGCTCCACCATAGACTGAATCAACTATTCAGCCCAGGGAGGGCCCTTCAGACTCCAC CACCCACCCGGTCCTCTGGCATCTGTGTAACAATCCAGGCTATCTGCATGGTGCCTGGGCAAACCAAGCA TGCCACGGGTCTGTGCTGCATGCCCTATCTGTACCATGAGTGTCTTTGGCTCCTTTCTGCTATGGGCAGG CCCCCAGAAAGGGCCAGGAGGGGGTGGCTAACTGTTCTCTCATAGGTTTGCCTACCCATTCCTCCCAGTA GGCCATCAAGAGGAGGTGGGCCTAGTTCCTTGGCATAAGCATAGTGAACACAAACGGGTGACTTTGGCAG TGCCAGCCTCCTCAACTTGTTCTAGGCTTTAGCAGGGGGCAGGAAGGAGGGGCTGGCCCCTCCTTAAAGA GTTGATATAAGTAAGACCTGGGTGTACTGGTGAGCAACCCCTCCCTCCATCCACAGACTCTACTGTACAT ATGGATTTTATGGTTGGCTTGGGGCAGCTGGGTTTGTCCTGGATATATGGTACTGTTATATAATAATAAT AATTATCATTATTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016758
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030321, AAH30321
• RefSeq Size: 2437 bp
• RefSeq ORF: 1644 bp
• Locus ID: 51791
• UniProt ID: P97492
• Cytogenetics: 13 29.8 cM
• Gene Summary: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o) alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.[UniProtKB/Swiss-Prot Function]