Zwilch (NM_026507) Mouse Untagged Clone

CAT#: MC204160

Zwilch (untagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (Zwilch), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zwilch
• Synonyms: 2310031L18Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027435
  CACGGCGGTATGTGGTCGCGAATGAACCGCGCGGCGGAGGAGTTTTATGCTCGTCTCCGGCAGGAATTTA ATGAAGAGAAGAAAGGAGCCTCTAAAGACCCATTCATCTATGAGGCTGATGTACAAGTGCAGTTGATCAG CAAAGGCCAACCAAGTCTTCTGAAAACTATTCTAAATGAAAATGACTCAGTGTTTCTCGTGGAAAAAGTG GTTTTGGAGAAGGAAGAAACAAGTCAAGTTGAAGAACTGCAGTCTGAAGAAACTGCTATTTCTGATCTCT CTGCTGGTGAAAATATTAGGCCACTGGCCTTACCTGTTGGGAGAGCAAGGCAGTTAATTGGGCTCTATAC CATGGCTCACAATCCTAATATGACTCATCTGAAGATTAAGCAGCCAGTGACAGCCCTTCCTCCTCTTTGG GTGAGATGTGACGGTTCAGATCCTGAAGGTACCTGTTGGCTAGGAGCTGAGCTTATCACAACAAATGACA TCATTGCAGGCGTTATCCTGTATGTGCTCACTTGTAAAGCTGATAAAAATTACTCTGAAGATCTGGAAAA TTTAAAAACTTCACACAAGAAAAGACATCACGTGTCTGCTGTGACAGCCAGAGGCTTTGCCCAGTATGAG CTCTTTAAGTCTGACGACTTGGATGACACTGTTGCCCCATCACAAACTACAGTCACGCTGGACCTTTCCT GGAGCCCTGTGGATGAGATGCTCCAAACCCCTCCCCTGTCTTCAACTGCAGCTCTGAATATCAGAGTACA ATCAGGAGAGTCCAGAGGTTGTCTGAGTCATCTTCACAGAGAACTGAAATTTCTCCTTGTTTTAGCTGAT GGCATCAGGACTGGTGTAACTGAATGGCTTGAGCCTCTGGAAACAAAATCTGCTCTTGAATTTGTTCAGG AATTTCTGAATGACTTAAATAAACTGGATGAATTTGATGATTCTACAAAAAAAGATAAACAAAAAGAGGC AGTGAACCATGACGCAGCTGCAGTTGTCAGGTCCATGCTTCTCACCGTGCGGGGGGATCTCGATTTTGCT GAACAGCTTTGGTGTAGAATGAGCAGCAGTGTGGTTTCCTATCAAGACTTGGTGAAGTGTTTCACACTCA TCCTGCAGAGTCTTCAGCGTGGTGATATACAGCCATGGTTGCACAGTGGAAGTAACAGTTTACTAAGTAA ACTCATTCATCAGTCTTACCATGGAGCTATGGACAGTGTCCCTCTCTCTGGAACTACCCCACTGCAGATG CTTTTGGAAATTGGTTTGGACAAACTGAAGAAAGATTATATCAGCTTTTTTGTCAGTCAGGAACTTGCAT CTTTGAATCATTTGGAATACTTCATTTCTCCCTCAGTATCGACACAGGAACAGGTTTGCCGTGTTCAGAA ACTCCATCATATTCTTGAAATCTTAGTCATCTGCATGCTTTTCATTAAACCTCAGCATGAGCTTCTCTTC TCTTTAACACAATCCTGCATAAAATATTATAAACAAAATCCTCTTGATGAGCAACACATTTTTCAGTTGC CAGTCAGACCAGCTGCTGTCAAGAATTTGTATCAAAGTGAGAAGCCGCAGAAATGGAGAGTGGAGCTCAG CAATAGTCAGAAGAGGGTGAAAACAGTGTGGCAACTGAGTGACAGCTCCCCTGTAGACCATTCCAGTTTC CACAGGCCTGAGTTTCCTGAGCTGACACTGAATGGTAGTCTGGAAGAAAGGACAGCCTTCGTTAACATGC TTACCTGCAGCCAGGTGCACTTCAAGTAATGCGCACTGCGGGCCCTTGCAAAGGGTCTTCTCTCCGCACA AAACAAAAAGGGGGAAAAGAGTGAGTTAATTTTCAGAGCCTGAAAAATAGACATCACGTGAACTTCTGCA GCAGAAAAGAAGAGTGATCCTGAAGAGATGCTTTGACAGATGAAGGTGGAGAGGAGATCCCGTCTCAGCC ACGTCTCCTGAGGAGAAGTGTCAGCATCAGGGCAGCAGTGAGGATGGAGAGGGCGCAGACTGTGGGGACC ACAATCTCTGAAGCCACGTGCATGTGCCTAAGCAAGGGCTCTGAAAGGGACAGATACATCCACCAGAAGT GAGCTGTGTTTTGAAACAAAAGCTAAAGGAGATGGGCCACTGCGTTTCTAATTTTTCTTATTTGTTGTAA AAGTGAACCAGTAAATCAAACATTATTTTGAAATGGAGTTTTGGAGCCATGGGACAAAGGCATCATGTTC ACTTGGTATGCACACAGCTTTTGGGAATCCTTACCATCACACAAAACTTACATCAGTGCTGGAAATGTAA CAGAAAATCTGGGGATGCCTGACTTTGGTTATTTCCCTGGTATTTTATTAAGCTTGAGTATGGTTAATAT TTATGCTGGCGTTGCATTAATCTCAAAAGATTAGCACCTATATTCCATGGATTCTCTCGGCTTTAGTCCA AATATTTTTAACTGGGCATGGCAGTACACACCTTTAATCCCAGCACTGAGGAGGCAGAGGCGGCCTGGTC TACAGAGCAAGTTCCAGAAAAACCACAGCTACACAGAGAAACCCTGTCTCAAAAGGAAAGACAAAAAGAA TCCCTGGGAAACATTTTCAGCTTTTTCAGTTTGTTATTCTCAATATACTTATATCCTCATATATTACAAT TTCTAATGGTAATTCTAATACTTATTTCTTTGTAAACACAAAAATTCACTAAAATGTTAAATTATTTGAT AAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026507
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027435, AAH27435
• RefSeq Size: 2745 bp
• RefSeq ORF: 1770 bp
• Locus ID: 68014
• UniProt ID: Q8R060
• Cytogenetics: 9 C
• Gene Summary: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (By similarity).[UniProtKB/Swiss-Prot Function]