Kdm8 (NM_029842) Mouse Untagged Clone

CAT#: MC204131

Kdm8 (untagged) - Mouse jumonji domain containing 5 (Jmjd5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm8
• Synonyms: 3110005O21Rik; Jmjd5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024807
  CTCGGTGTAGTCGGTGATAAAGCCAGCCGAAGAATCTTCGCCCGGACGGAACCAGTCGTCGCCAGCAACG AGAGACACAAACAAGTCCAAGCGCCAGAGAGGTGCTGATCATGTCAGAGGACACCACAGAGCCCCTGGTG GGGTCCAGCACCCTCTGGAAGGAGCTCAGGACGCTCCTGCCTGACAAAGAGGAGGAGCTGAAGCTGGACC TCGGTGAGAAGGTGGACAGGAGCGTGGCTGCGCTTTTGCGGCAGGCTGTGGGTCTATTCTACGCGGGCCA TTGGCAGGGGTGCCTGCAGGCCAGTGAGGCCGTCCTAGACTACTCCTGGGAAAAGCTCAACACTGGTCCC TGGAGAGATGTAGACAAGGAGTGGCGCCGGGTATATTCCTTCGGCTGCCTCCTGAAGGCCCTGTGCCTGT GCCAGGCACCACAGAAGGCCACTACTGTGGTGGAAGCACTGCGGGTGTGTGACATGGGCCTGTTGATGGG GGCAGCCATCCTCGAGGACATTCTTCTCAAAGTTGTCGCAGTCCTCCAGACACACCAGCTCCCAGGAAAG CAGCCTGCCCGCGGCCCCCACCAGGATCAGCCTGCCACCAAGAAGGCAAAGTGTGACGCCAGCCCAGCTC CTGATGTCATGTTAGAGCGGATGGTACCCAGGCTCCGCTGCCCACCTCTGCAGTACTTCAAGCAGCATTT TCTAGTTCCTGGGAGGCCTGTGATCTTGGAAGGTGTGGCCGACCACTGGCCGTGCATGAAGAAGTGGAGT CTGCAGTACATCCAGGAGATTGCTGGCTGCCGCACCGTCCCCGTGGAAGTGGGCTCAAGGTACACAGATG AAGACTGGTCCCAGACTCTCATGACAGTCGATGAGTTCATCCAGAAGTTCATCCTGAGCGAGGCAAAGGA TGTCGGGTACCTTGCTCAGCACCAGCTCTTCGACCAGATCCCAGAGTTGAAGCGGGACATCAGCATCCCT GATTACTGTTGCCTGGGCAATGGAGAAGAGGAAGAGATCACTATCAATGCCTGGTTTGGTCCACAAGGCA CCATCTCCCCACTGCATCAGGACCCCCAGCAGAACTTCCTGGTCCAGGTGCTAGGAAGGAAGTACATCCG ATTGTATTCGCCGCAAGAGTCTGAGGCAGTGTACCCTCACGAGACGCACATTCTTCATAACACCAGCCAG GTTGATGTGGAAAACCCCGACCTTGAGAAGTTTCCCAAGTTCACTGAGGCCCCATTCCTGTCCTGCATTC TGTCCCCAGGAGACACCCTCTTTATACCTGCTAAGTACTGGCATTATGTGCGCTCCCTGGACCTGAGCTT CTCTGTCAGCTTCTGGTGGTCATAGCTAGGACCAGACTGGAAAGGACCTCTGGGCAGACACCTTACCCAA AGGCAGTAAACCAAGTGTCAGTGTGGGCCCTGCATGCCGCTAGACTCCAGCAGCCCTGCCCCCAGCCAAT GTCTGTTGTGTGACAGAGCTGCTATGTACCCACGTGTGGCCCACCTGCTGTGTGTGCCCGCGTGTGTAGG GCAGAGCTGCTACATGTACCTAAGCTATCCAGATTATGGGTGCTTTTTTTCCTTTGTAACTGCTGGGCTG AGTATCCCTTGCTGCTAGGTGCCCAAGGGCAGCTGGCAAGCTGCTCAGAGACAGAACACAACCTGTAATA GGGGTTTTCTTGGGTGGAATATAGCCGGGTCTGGGACAGCTGCTGTGGTCCCAGTCTTCTGTGTACCCAG GCCTCACTGCCAGCTTACTACTAGGAGTTGGAAACAGAGGTCCTTGGCTCCATACTTCCCCTCGGGGCAC TTATGCACCAGGCTGGAAGAGGAGGGCAGCGGAACTACTTTGTTGTGAATGTGGGGAATACAGAGAAGAG CGAGGTCTTCCCAGAATATATAGGAGCGGCCTTTTACAAGGAAGCCCCTGCCCCCAAGCCTCCTTGATGA AGAGATGGTTCTTACGTTCAAACTGTTTTAGTTGATGGCGGATGTGCATGCACACACCTTACTCAGGGTG AATCAAGGAGTAAATACCTCTTGTGGGAAGGACTGCCCAGGAAGGGAAGCTCATTGGCTGAACACTCGGG GCCCACTTAGATACCACATTACCATGGAGAACTCCAGGTTTTTATGTTACAAGACTGACTGTCACCGTCC TCTCAGTGGGGTGTCATGTTCCAGATCAGGTAGTTTGGCAGGGAGCTGGCTCTACTCCAGCTGTTCTCAA TTCTGAGATTTCCCCAGTCTGAGCCTGCTCAACCTTACCAGTTCTTCTGTCTGGTGGCCCGGTCCACTTG CCCCATATGTAACTTAAATATTTTCAATAACAATAAAATATATTTTATTAATTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029842
• Insert Size: 1245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024807, AAH24807
• RefSeq Size: 2378 bp
• RefSeq ORF: 1245 bp
• Locus ID: 77035
• UniProt ID: Q9CXT6
• Cytogenetics: 7 F3
• Gene Summary: Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage. Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established. Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation. Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition. Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation. Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition (By similarity). Regulates the circadian gene expression in the liver (PubMed:30500822). Represses the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a catalytically-independent manner (By similarity). Negatively regulates the protein stability and function of CRY1; required for AMPK-FBXL3-induced CRY1 degradation (PubMed:30500822).[UniProtKB/Swiss-Prot Function]