Sptssb (NM_133675) Mouse Untagged Clone

CAT#: MC204100

Sptssb (untagged) - Mouse RIKEN cDNA 1110032A04 gene (1110032A04Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sptssb
• Synonyms: 1110032A04Rik; ADMP; Sssptb
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC022628
  GCTGGGGAGAAGCCAGAGCATCTAAGCTCACTCTAAGGCAGGAGACGAAGAAATAAAGCCCAGAGAGGAG AAATAATCTACACCAGATTGTTCAGAGGAAAAGCATCACCGAGTACCAAGCCTGGATACTCCTGCACAGG CAGACTTTTCCCAGCTTAAGGAAGAGCCATGGATTTCAAGCGCGTGAAGGAGTATTTTGCCTGGCTCTAT TATCAATACCAGATCATCACCTGCTGTGCTGTCATGGAGCCTTGGGAACAATCGATGCTCAACACCATCA TACTGACCATTGTGGCTATGGTGGTATACACTGCCTATGTCTTCATCCCCATCCACATCCGCCTGGCTTG GGAATTTTTCTCCAAAATATGTGGCTATGACAGTTCCATTTCTAACTGATCTCATCTGAATTCGGTGAAA GGGCATAACGTGTTCACATTGCTTACAACTTACTGACTTGGGTGACCGTATCATCTTTCACCTCTGACCT GACCAGCACTCTCTCTATGCACTGTCCCGCACATCCTGCCTGGAGTATGAGATAGGTTTTCCCCCACTTG CTTTTGTACATGCAATATTGTGCATCAGGTGATAGAAAAGGCGATGACTTTAACCCCATAGCCCACATTT GCTCAACTCTATGGCTTTAGGTTTCTTTTGAAATCAGGATGCTTTGAAATAAAACTTTTCTGGAAAATAG GCTTATAAGAGACATGACAAAGTCATATTTACATTACTCTCTTCATATTATTCTATACTAAAGGATATGT TACAAAAGTATTCTTTATAGGCTACTGTCAAAAGTACCCTGTACTTGTTTACAATTTATTAAAAGATGGG AATAAATAGATGAACCTTTAAAGTCTTATTTTCTGGGAAATAGCTAATACCTAAAATTCGTTTCTTCTTC TTTTTTAAATTTTCATTTTCTTTGCTTTAGCTAAAATTCTTTTAGTTTAAAGTAACAGGCCAGAGACAGT TATCTTTTCTTCAGATTTTGTTTCAGCATCGTTTCAAATGCTCCTGAAATCTAGCTTTATTACAGCTTTA AAGGAATGACTTTTTTTCCCCCTAGGTAAACAAATAACTATTATACGAGAGAATAATTTAAGTTTAAGAA ACAAAGGATAATCAAAGCAAATTCAGATACTGTGACTTTGGATCACGCCCCACTTTACATGATGCTGGAG ACAGAAGAAGAGAGATTGTTGTTTTGCTGTGCCTTCAGTGAAAGGAAGGGAAGCTGTGTGTCCTAGGCTA CAGGAAGAAAAGCTAATAAATAGGCTGGAAGTAATAGCCAGGCAGCAGGGAGTCGGGAGCTCCAGTTAAA GGCTTTGATATAGCAGCTCCTTTGCACAGCAGAAACAAAATTTATTAAATTTCTCTCAAAATGTTTATCT TCAAAGCAGATGTAAGATTTTAATTATAGTGCTGAGTCAAACATAAAAGCCAAAGACCAAGCCTTGCAGC TTTTCTTTCCTCTCAATAAATATTAACATTAGTGATTCTGGTAAAATAAATAAATAAATGAACCGGCTTT GGACAATATTGGCACCTTTGTTTTAGGAGAGAAATCTTCCAAGTAGACATCCAAAGAAAGTTATTTGGCA CGCAAAAATCAACCTGCATGTCTGTTTAAAAAAACAAAATGTACATTTTAAAGAGAAACCAGATATGAAA TATATACTTACTGATTTTTGCAAAATAAAATGAAGTTTACTTTTATAAGCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAG
• Restriction Sites: RsrII-NotI
• ACCN: NM_133675
• Insert Size: 231 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022628, AAH22628
• RefSeq Size: 1765 bp
• RefSeq ORF: 231 bp
• Locus ID: 66183
• UniProt ID: Q925E8
• Cytogenetics: 3 E2
• Gene Summary: Stimulates the activity of serine palmitoyltransferase (SPT). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference, complexes with this subunit showing a clear preference for longer acyl-CoAs. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.