Bbs7 (NM_027810) Mouse Untagged Clone

CAT#: MC204070

Bbs7 (untagged) - Mouse Bardet-Biedl syndrome 7 (human) (Bbs7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs7
• Synonyms: 8430406N16Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031505
  GGGCTCTCCGAGGGAAGGCTTCCACAGCGGGTCCCGCCTCTCCGTGCCGCCGCCATGGATCTGACGTTAA GCCGGGCAGACTACCTCCAGGTCGGAGTGACCTCTCAGAAAACTATGAAGTTGCTTCCTACCTCAAGACA AAGGGCTACACAAAAGGTGGTTGTTGGAGATCAAGATGGGGTAGTGATATGCTTCGGCGTGAAGAAAGGA GAAGCTGTGCCAGTGTTCAAGACTTTACCAGGGCAGAAGATCTCGAGGCTGGAACTAGGAGGGGCCGTCA ACACGCCTCAGGAGAAAATCTTCATTGCTGCAGGGTCCGAGATTAGAGGCTTTACAAAGAGAGGGAAACA GTTTCTCTCTTTTGAAACTAACCTCACGGAAAGCATTAAAGCTATGTACATATCTGGCTCTGACCTCTTC CTCAGTGCAAGCTACATCTATAATCACTACTGTGATTGCAAAGACCAAAATTATTACCTTTCTGGGGACA AAATCAATGATGTCATCTGCCTTCCTGTGGAAAAGTTATCTCGCGTGACACCGGTGTTGGCCTGCCAGGA CCGGGTGCTCCGAGTCTTACAGGGATCTGATGTGATGTATGAAATTGAGGTTCCTGGTCCACCCACCGTC TTAGCACTGCATAATGGAGATGGAGGTGACTCTGGAGAAGGACTTCTGTTCGGCACATCAGATGGAAGAC TTGGGCTTATTCAGATCACAACATCCAAACCAATACACAAATGGGAAATTCGAAATGACAAAAAGCGGGG AGGTATCTTGTGTGTTGACAGCTTTGATATCATGGGTGATGGGGTTAAAGATTTACTTGTTGGGAGAGAT GATGGAATGGTGGAAGTATATAGTTTTGAAAATGCAAATGAACCTGTGCTACGATTTGATCAGATGCTGT CTGAAAGCGTCACCTCCATCCAGGGTGGCTGTGTGGGAAAAGATGGCTATGATGAAATCGTGCTGGCCAC ATATTCAGGCTGGGTGACAGGGCTGACTACAGAGCCAACTCATAAAGAGAGTGGACCAGGAGAAGAACTA AAACTTAATCAGGAGATGCAGAATAAGATTTCTTCTTTAAGGTCTGAAATAGAACACCTGCAGTTTAAGG TGCTTCAGGAGAGAGAAAATTACCAACAGTCTTCACAGTCAAGCCAAGCAAAGTCAACAGTCCCTTCATT CAGTATAAATGACAAGTTCACGTTGAATAAAGAAGATGCTAGCTACAGCCTCGTCTTAGAGGTGCGGACT GCAATAGACAATGTCCTAATACAGAGTGATGTTCCAATAGATCTACTTGATGTGGACAAGAATTCTGCTG TTGTGAGCTTCAGCAGCTGTGACACAGAGTCAAACGACAACTTTCTTCTTGCTACTTATCGGTGCCAGGC AAATACCACCAGGCTGGAGCTCAAGATTCGTTCAATTGAAGGCCAGTATGGCACACTCCAGGCATATGTG ACTCCAAGAATCCAACCAAAAACCTGTCAGGTTCGCCAATACCACATCAAACCTCTATCACTCCATCAAA GAACTCACTTTATTGACCATGACAGGCCTATGAACACACTGACTCTAACAGGCCAGTTCAGCTTTGCTGA AGTTCACTCCTGGGTGGTGTTCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGCAGGGGAATGTGCAACA TTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATACAGGAAAGGAGAAGGAGTTTTTA AATCTGACAACATTTCTACAATATCCATCCTAAAAGATGTGCTTTCTAAAGAAGCTACAAAAAGGAAAAT TAATCTCAACATATCATACGAGATAAACGAGGTGTCTGTCAAGCACACTTTAAAGCTAATTCACCCGAAG CTGGAGTATCAGCTGCTTCTGGCTAAGAAAGTGCAGTTAATCGATGCCCTGAAGGAATTGCAGGTTCATG AGGGAAATACAGACTTTCTGACTCCAGAGTACCGATGTATTCTAGAAGAGGCAGATCATCTACAAGAGGA ATACAAAAAGCAGCCTGCACATCTTGAAAGACTCTATGGTATGATTACTGATCTTTTTATAGATAAATTT AAGTTCAAAGGCACTAATGTAAAAACCAAAGTACCCATGCTCCTGGAGATTCTCGACAGCTACGACCAGA ACACACTGATTTCTTTCTTCGATGCAGCATGAAAGATAAACAAGGTAGGCCAACAGGACAGCCGAAGAAG CAAGCAGAGCCTGATCAGACCCGAGGCACCTCAGATGGACGTCCCACATGTAATCAGAAGAGTGACGGCC TCTAAGCATCCCCCTACGAGTACCCATGAAAGTCCTTTTTATTGTGTACTTGAGGGGAAAAGAAGATCTT TTAGTCATTCATTTCAGGGGAATATTGCATTGAAAGTTTTAGAATACTATTAAATAAGAGCAAATAAGCT GTCTTTTGTTTTCAAAGGGTAGTTATGAAAACAATGATTACATTTTATTATATTTTATGTATGATTCTTA ACTATTAAATGTGAACATTTAAGGTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027810
• Insert Size: 2148 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031505, AAH31505
• RefSeq Size: 2602 bp
• RefSeq ORF: 2148 bp
• Locus ID: 71492
• UniProt ID: Q8K2G4
• Cytogenetics: 3 B
• Gene Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly (By similarity).[UniProtKB/Swiss-Prot Function]