Krtcap2 (NM_025327) Mouse Untagged Clone

CAT#: MC204068

Krtcap2 (untagged) - Mouse keratinocyte associated protein 2 (Krtcap2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Krtcap2
• Synonyms: 0610010I12Rik; Kcp2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031492
  CACGGATTGGACCACGGACGGGGCGTGATGGTAGTCGGTACCGGGACCTCCTTGGCGCTGTCCTCCCTCC TGTCCCTGCTGCTCTTCGCTGGCATGCAGATCTACAGCCGCCAGCTGGCCTCCACCGAGTGGCTCACCAT TCAGGGGGGCCTGCTGGGCTCCGGCCTTTTCGTCTTCTCCCTGACTGCCTTCAATAATCTGGAGAATCTG GTCTTTGGTAAAGGCTTCCAAGCAAAAATCTTCCCTGAGATTCTCCTCTGCTTGCTGTTGGCGCTCTTTG CCTCCGGCCTCATCCACCGAGTCTGTGTCACCACTTGCTTCATCTTCTCCATGGTTGGTCTGTACTACAT CAACAAGATATCGTCCACTCTGTACCAGGCAACAGCTCCAGTTCTCACACCGGCCAAAATCACTGGCAAG GGCAAGAAGAGAAACTAACTGTGAATGAGTAATAAAGTTGTGTCTTTGTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025327
• Insert Size: 411 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031492, AAH31492
• RefSeq Size: 502 bp
• RefSeq ORF: 411 bp
• Locus ID: 66059
• UniProt ID: Q5RL79
• Cytogenetics: 3 F1
• Gene Summary: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).